The involvement of thaumatin-like proteins in plant food cross-reactivity: a multicenter study using a specific protein microarray

Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-r...

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Detalles Bibliográficos
Autores: Palacín, Arantxa, Rivas, Luis A., Gómez-Casado, Cristina, Aguirre, Jacobo, Tordesillas, Leticia, Bartra, Joan, Carrillo, Teresa, Cuesta-Herranz, Javier, Cumplido Bonny, José A., Flores, Enrique, García-Alvarez-Eire, Mar G., García-Nuñez, Ignacio, Fernández, Francisco J., Gamboa, Pedro, Muñoz, Rosa, Sánchez-Monge, Rosa, Torres, Maria, Varela Losada, Susana, Villalba, Mayte, Parro, Victor, Blanca, Miguel, Salcedo, Gabriel, Díaz-Perales, Araceli
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17041
Acceso en línea:http://hdl.handle.net/20.500.12105/17041
Access Level:acceso abierto
Palabra clave:España
Geografía
Reacciones cruzadas
Hipersensibilidad a los alimentos
Inmunización
Inmunoensayo
Inmunoglobulina E
Peso molecular
Proteínas de plantas
Polen
Análisis por matrices de proteínas
Adolescent
Adult
Child
Humans
Female
Male
Young Adult
Spain
Cross Reactions
Food Hypersensitivity
Food
Fruit
Geography
Middle Aged
Immunization
Immunoassay
Immunoglobulin E
Molecular Weight
Plant Proteins
Pollen
Protein Array Analysis
Descripción
Sumario:Cross-reactivity of plant foods is an important phenomenon in allergy, with geographical variations with respect to the number and prevalence of the allergens involved in this process, whose complexity requires detailed studies. We have addressed the role of thaumatin-like proteins (TLPs) in cross-reactivity between fruit and pollen allergies. A representative panel of 16 purified TLPs was printed onto an allergen microarray. The proteins selected belonged to the sources most frequently associated with peach allergy in representative regions of Spain. Sera from two groups of well characterized patients, one with allergy to Rosaceae fruit (FAG) and another against pollens but tolerant to food-plant allergens (PAG), were obtained from seven geographical areas with different environmental pollen profiles. Cross-reactivity between members of this family was demonstrated by inhibition assays. Only 6 out of 16 purified TLPs showed noticeable allergenic activity in the studied populations. Pru p 2.0201, the peach TLP (41%), chestnut TLP (24%) and plane pollen TLP (22%) proved to be allergens of probable relevance to fruit allergy, being mainly associated with pollen sensitization, and strongly linked to specific geographical areas such as Barcelona, Bilbao, the Canary Islands and Madrid. The patients exhibited >50% positive response to Pru p 2.0201 and to chestnut TLP in these specific areas. Therefore, their recognition patterns were associated with the geographical area, suggesting a role for pollen in the sensitization of these allergens. Finally, the co-sensitizations of patients considering pairs of TLP allergens were analyzed by using the co-sensitization graph associated with an allergen microarray immunoassay. Our data indicate that TLPs are significant allergens in plant food allergy and should be considered when diagnosing and treating pollen-food allergy.

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