Mechanical control of epithelial tissue formation and function in early development

The outer epithelial layer is the first cell type that emerges during development, providing essential mechanical protection and is indispensable for morphogenesis. Here, we study how the interplay between molecular and mechanosensing pathways participate in this tissue formation and function by com...

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Detalles Bibliográficos
Autor: Tolosa Ramon, Queralt
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/688969
Acceso en línea:http://hdl.handle.net/10803/688969
Access Level:acceso abierto
Palabra clave:Mechanobiology
Embryonic epithelium
Cellular shape
Cellular contractility
Specification
Mecanobiologia
Epiteli embrionari
Forma cel·lular
Contractilitat cel·lular
Especificació
576
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spelling Mechanical control of epithelial tissue formation and function in early developmentTolosa Ramon, QueraltMechanobiologyEmbryonic epitheliumCellular shapeCellular contractilitySpecificationMecanobiologiaEpiteli embrionariForma cel·lularContractilitat cel·lularEspecificació576The outer epithelial layer is the first cell type that emerges during development, providing essential mechanical protection and is indispensable for morphogenesis. Here, we study how the interplay between molecular and mechanosensing pathways participate in this tissue formation and function by combining in vivo assays with bottom-up ex vivo/in vitro assays using zebrafish enveloping layer (EVL) as model system. We found that mechanosensitive signalling pathways involving Trpv4 (transient receptor potential cation channel subfamily V member 4) activity at the plasma membrane and cPLA2 (cytoplasmic phospholipase A2) activity at the inner nuclear membrane, together with the transcription factors CCAAT/enhancer-binding protein-α (C/EBPα) and -β (C/EBPβ), are involved in regulating EVL cellular/nuclear shape, contractility and specification, thus affecting early development when perturbed. Moreover, we established ex vivo and in vitro systems that are able to recapitulate de novo EVL formation.La capa epitelial externa és el primer tipus cel·lular que emergeix durant el desenvolupament, proporcionant una protecció mecànica essencial i sent indispensable per a la morfogènesi. En aquest estudi, investiguem com la interacció entre vies moleculars i mecano-sensitius participa en la formació i funció d'aquest teixit mitjançant l'ús combinat de proves in vivo i ex vivo/in vitro utilitzant l’enveloping layer (EVL) del peix zebra com a sistema model. Hem descobert que les vies de senyalització mecano-sensibles que involucren l'activitat del Trpv4 (canal catiónic de potencial receptor transitori de la subfamília V) a la membrana plasmàtica i l'activitat del cPLA2 (fosfolipasa A2 citoplasmàtica) a la membrana nuclear interna, juntament amb els factors de transcripció CCAAT/enhancer-binding protein-α (C/EBPα) i -β (C/EBPβ), estan implicats en la regulació de la forma cel·lular/nuclear, contractilitat i especificació de l'EVL, afectant així el desenvolupament primerenc quan es pertorben. A més, hem establert sistemes ex vivo i in vitro que són capaços de recapitular la formació de l'EVL de novo.Programa de Doctorat en BiomedicinaUniversitat Pompeu FabraRuprecht, VerenaUniversitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida202320252023info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersion218 p.application/pdfhttp://hdl.handle.net/10803/688969TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésL'accés als continguts d'aquesta tesi queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc/4.0/http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/6889692026-06-14T12:46:07Z
dc.title.none.fl_str_mv Mechanical control of epithelial tissue formation and function in early development
title Mechanical control of epithelial tissue formation and function in early development
spellingShingle Mechanical control of epithelial tissue formation and function in early development
Tolosa Ramon, Queralt
Mechanobiology
Embryonic epithelium
Cellular shape
Cellular contractility
Specification
Mecanobiologia
Epiteli embrionari
Forma cel·lular
Contractilitat cel·lular
Especificació
576
title_short Mechanical control of epithelial tissue formation and function in early development
title_full Mechanical control of epithelial tissue formation and function in early development
title_fullStr Mechanical control of epithelial tissue formation and function in early development
title_full_unstemmed Mechanical control of epithelial tissue formation and function in early development
title_sort Mechanical control of epithelial tissue formation and function in early development
dc.creator.none.fl_str_mv Tolosa Ramon, Queralt
author Tolosa Ramon, Queralt
author_facet Tolosa Ramon, Queralt
author_role author
dc.contributor.none.fl_str_mv Ruprecht, Verena
Universitat Pompeu Fabra. Departament de Medicina i Ciències de la Vida
dc.subject.none.fl_str_mv Mechanobiology
Embryonic epithelium
Cellular shape
Cellular contractility
Specification
Mecanobiologia
Epiteli embrionari
Forma cel·lular
Contractilitat cel·lular
Especificació
576
topic Mechanobiology
Embryonic epithelium
Cellular shape
Cellular contractility
Specification
Mecanobiologia
Epiteli embrionari
Forma cel·lular
Contractilitat cel·lular
Especificació
576
description The outer epithelial layer is the first cell type that emerges during development, providing essential mechanical protection and is indispensable for morphogenesis. Here, we study how the interplay between molecular and mechanosensing pathways participate in this tissue formation and function by combining in vivo assays with bottom-up ex vivo/in vitro assays using zebrafish enveloping layer (EVL) as model system. We found that mechanosensitive signalling pathways involving Trpv4 (transient receptor potential cation channel subfamily V member 4) activity at the plasma membrane and cPLA2 (cytoplasmic phospholipase A2) activity at the inner nuclear membrane, together with the transcription factors CCAAT/enhancer-binding protein-α (C/EBPα) and -β (C/EBPβ), are involved in regulating EVL cellular/nuclear shape, contractility and specification, thus affecting early development when perturbed. Moreover, we established ex vivo and in vitro systems that are able to recapitulate de novo EVL formation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10803/688969
url http://hdl.handle.net/10803/688969
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 218 p.
application/pdf
dc.publisher.none.fl_str_mv Universitat Pompeu Fabra
publisher.none.fl_str_mv Universitat Pompeu Fabra
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422458810925056
score 15.301603

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