Mechanical control of epithelial tissue formation and function in early development
The outer epithelial layer is the first cell type that emerges during development, providing essential mechanical protection and is indispensable for morphogenesis. Here, we study how the interplay between molecular and mechanosensing pathways participate in this tissue formation and function by com...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/688969 |
| Acceso en línea: | http://hdl.handle.net/10803/688969 |
| Access Level: | acceso abierto |
| Palabra clave: | Mechanobiology Embryonic epithelium Cellular shape Cellular contractility Specification Mecanobiologia Epiteli embrionari Forma cel·lular Contractilitat cel·lular Especificació 576 |
| Sumario: | The outer epithelial layer is the first cell type that emerges during development, providing essential mechanical protection and is indispensable for morphogenesis. Here, we study how the interplay between molecular and mechanosensing pathways participate in this tissue formation and function by combining in vivo assays with bottom-up ex vivo/in vitro assays using zebrafish enveloping layer (EVL) as model system. We found that mechanosensitive signalling pathways involving Trpv4 (transient receptor potential cation channel subfamily V member 4) activity at the plasma membrane and cPLA2 (cytoplasmic phospholipase A2) activity at the inner nuclear membrane, together with the transcription factors CCAAT/enhancer-binding protein-α (C/EBPα) and -β (C/EBPβ), are involved in regulating EVL cellular/nuclear shape, contractility and specification, thus affecting early development when perturbed. Moreover, we established ex vivo and in vitro systems that are able to recapitulate de novo EVL formation. |
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