Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress

Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential fo...

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Autores: Palomino Ruiz-Poveda, Olga María, Giordiani, Veronica, Chowen, Julie, Fernández Alfonso, María Soledad, Goya, Luis
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/110235
Acceso en línea:https://hdl.handle.net/20.500.14352/110235
Access Level:acceso abierto
Palabra clave:EA.hy926
Free fatty acids
Reactive oxygen species
Cardiovascular disease
Farmacología (Farmacia)
3209 Farmacología
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spelling Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative StressPalomino Ruiz-Poveda, Olga MaríaGiordiani, VeronicaChowen, JulieFernández Alfonso, María SoledadGoya, LuisEA.hy926Free fatty acidsReactive oxygen speciesCardiovascular diseaseFarmacología (Farmacia)3209 FarmacologíaOxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential for cell and tissue function and olive oil free fatty acids have proved to exhibit beneficial effects on risk factors for cardiovascular disease. We hypothesize that realistic concentrations within the physiological range of oleic (OA) and palmitic (PA) acids could be beneficial in the prevention of oxidative stress in vascular endothelium. Hence, pre-treatment and co-treatment with realistic physiological doses of palmitic and oleic acids were tested on cultured endothelial cells submitted to a chemically induced oxidative stress to investigate their potential chemo-protective effect. Cell viability and markers of oxidative status: reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated. As a conclusion, the increased ROS generation induced by stress was significantly prevented by a pre- and co-treatment with PA or OA. Moreover, pre- and co-treatment of cells with FFAs recovered the stress-induced MDA concentration to control values and significantly recovered depleted GSH and normalized GPx and GR activities. Finally, pre- and co-treatment of cells with physiological concentrations of PA or OA in the low micromolar range conferred a substantial protection of cell viability against an oxidative insult.MDPIUniversidad Complutense de Madrid20222022-08-1620222022-08-16journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/110235reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-82565-C2-2-R CROSSTALK ENTRE LOS ASTROCITOS HIPOTALAMICOS Y EL TEJIDO ADIPOSO PERIVASCULAR EN EL METABOLISMO ENERGETICO Y LA FUNCION CARDIOVASCULAR: IMPACTO DE MODIFICACIONES EN LA DIETAopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1102352026-06-02T12:44:21Z
dc.title.none.fl_str_mv Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
title Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
spellingShingle Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
Palomino Ruiz-Poveda, Olga María
EA.hy926
Free fatty acids
Reactive oxygen species
Cardiovascular disease
Farmacología (Farmacia)
3209 Farmacología
title_short Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
title_full Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
title_fullStr Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
title_full_unstemmed Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
title_sort Physiological Doses of Oleic and Palmitic Acids Protect Human Endothelial Cells from Oxidative Stress
dc.creator.none.fl_str_mv Palomino Ruiz-Poveda, Olga María
Giordiani, Veronica
Chowen, Julie
Fernández Alfonso, María Soledad
Goya, Luis
author Palomino Ruiz-Poveda, Olga María
author_facet Palomino Ruiz-Poveda, Olga María
Giordiani, Veronica
Chowen, Julie
Fernández Alfonso, María Soledad
Goya, Luis
author_role author
author2 Giordiani, Veronica
Chowen, Julie
Fernández Alfonso, María Soledad
Goya, Luis
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv EA.hy926
Free fatty acids
Reactive oxygen species
Cardiovascular disease
Farmacología (Farmacia)
3209 Farmacología
topic EA.hy926
Free fatty acids
Reactive oxygen species
Cardiovascular disease
Farmacología (Farmacia)
3209 Farmacología
description Oxidative stress has been proposed to be a pathogenic mechanism to induce endothelial dysfunction and the onset of cardiovascular disease. Elevated levels of free fatty acids can cause oxidative stress by increasing mitochondrial uncoupling but, at physiological concentrations, they are essential for cell and tissue function and olive oil free fatty acids have proved to exhibit beneficial effects on risk factors for cardiovascular disease. We hypothesize that realistic concentrations within the physiological range of oleic (OA) and palmitic (PA) acids could be beneficial in the prevention of oxidative stress in vascular endothelium. Hence, pre-treatment and co-treatment with realistic physiological doses of palmitic and oleic acids were tested on cultured endothelial cells submitted to a chemically induced oxidative stress to investigate their potential chemo-protective effect. Cell viability and markers of oxidative status: reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA), glutathione peroxidase (GPx) and glutathione reductase (GR) were evaluated. As a conclusion, the increased ROS generation induced by stress was significantly prevented by a pre- and co-treatment with PA or OA. Moreover, pre- and co-treatment of cells with FFAs recovered the stress-induced MDA concentration to control values and significantly recovered depleted GSH and normalized GPx and GR activities. Finally, pre- and co-treatment of cells with physiological concentrations of PA or OA in the low micromolar range conferred a substantial protection of cell viability against an oxidative insult.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-08-16
2022
2022-08-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/110235
url https://hdl.handle.net/20.500.14352/110235
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 BFU2017-82565-C2-2-R CROSSTALK ENTRE LOS ASTROCITOS HIPOTALAMICOS Y EL TEJIDO ADIPOSO PERIVASCULAR EN EL METABOLISMO ENERGETICO Y LA FUNCION CARDIOVASCULAR: IMPACTO DE MODIFICACIONES EN LA DIETA
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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