Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures

The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, w...

Descripción completa

Detalles Bibliográficos
Autores: Castanera Andrés, Raúl, Pérez Garrido, María Gumersinda, Omarini, Alejandra, Alfaro Sánchez, Manuel, Pisabarro de Lucas, Gerardo, Faraco, Vicenza, Amore, Antonella, Ramírez Nasto, Lucía
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/31946
Acceso en línea:https://hdl.handle.net/2454/31946
Access Level:acceso abierto
Palabra clave:Pleurotus ostreatus
Submerged fermentation
Solid fermentation
Laccase gene transcription
id ES_e2abecd2bdcd64ee8ab25569255a2a45
oai_identifier_str oai:academica-e.unavarra.es:2454/31946
network_acronym_str ES
network_name_str España
repository_id_str
spelling Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation culturesCastanera Andrés, RaúlPérez Garrido, María GumersindaOmarini, AlejandraAlfaro Sánchez, ManuelPisabarro de Lucas, GerardoFaraco, VicenzaAmore, AntonellaRamírez Nasto, LucíaPleurotus ostreatusSubmerged fermentationSolid fermentationLaccase gene transcriptionThe genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.This work was supported by funds from project AGL2008-05608-C02-01 of the Spanish National Research Plan, from Bioethanol-Euroinnova, and from the Autonomous Government of Navarre and by additional institutional support from the Public University of Navarre.American Society for MicrobiologyProducción AgrariaNekazaritza EkoizpenaGobierno de Navarra / Nafarroako GobernuaUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/31946reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2012, American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/319462026-06-17T12:41:47Z
dc.title.none.fl_str_mv Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
title Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
spellingShingle Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
Castanera Andrés, Raúl
Pleurotus ostreatus
Submerged fermentation
Solid fermentation
Laccase gene transcription
title_short Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
title_full Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
title_fullStr Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
title_full_unstemmed Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
title_sort Transcriptional and enzymatic profiling of Pleurotus ostreatus laccase genes in submerged and solid-state fermentation cultures
dc.creator.none.fl_str_mv Castanera Andrés, Raúl
Pérez Garrido, María Gumersinda
Omarini, Alejandra
Alfaro Sánchez, Manuel
Pisabarro de Lucas, Gerardo
Faraco, Vicenza
Amore, Antonella
Ramírez Nasto, Lucía
author Castanera Andrés, Raúl
author_facet Castanera Andrés, Raúl
Pérez Garrido, María Gumersinda
Omarini, Alejandra
Alfaro Sánchez, Manuel
Pisabarro de Lucas, Gerardo
Faraco, Vicenza
Amore, Antonella
Ramírez Nasto, Lucía
author_role author
author2 Pérez Garrido, María Gumersinda
Omarini, Alejandra
Alfaro Sánchez, Manuel
Pisabarro de Lucas, Gerardo
Faraco, Vicenza
Amore, Antonella
Ramírez Nasto, Lucía
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producción Agraria
Nekazaritza Ekoizpena
Gobierno de Navarra / Nafarroako Gobernua
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
dc.subject.none.fl_str_mv Pleurotus ostreatus
Submerged fermentation
Solid fermentation
Laccase gene transcription
topic Pleurotus ostreatus
Submerged fermentation
Solid fermentation
Laccase gene transcription
description The genome of the white rot basidiomycete Pleurotus ostreatus includes 12 phenol oxidase (laccase) genes. In this study, we examined their expression profiles in different fungal strains under different culture conditions (submerged and solid cultures) and in the presence of a wheat straw extract, which was used as an inducer of the laccase gene family. We used a reverse transcription- quantitative PCR (RT-qPCR)-based approach and focused on determining the reaction parameters (in particular, the reference gene set for the normalization and reaction efficiency determinations) used to achieve an accurate estimation of the relative gene expression values. The results suggested that (i) laccase gene transcription is upregulated in the induced submerged fermentation (iSmF) cultures but downregulated in the solid fermentation (SSF) cultures, (ii) the Lacc2 and Lacc10 genes are the main sources of laccase activity in the iSmF cultures upon induction with water-soluble wheat straw extracts, and (iii) an additional, as-yet-uncharacterized activity (Unk1) is specifically induced in SSF cultures that complements the activity of Lacc2 and Lacc10. Moreover, both the enzymatic laccase activities and the Lacc gene family transcription profiles greatly differ between closely related strains. These differences can be targeted for biotechnological breeding programs for enzyme production in submerged fermentation reactors.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/31946
url https://hdl.handle.net/2454/31946
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2012, American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2012, American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422431703138304
score 15,81155

Similares en LA Referencia