Estudio histológico del efecto de la aplicación de tricostatina durante la inducción de embriogénesis en microsporas de Brassica napus
[EN] Microspore culture is a useful tool to produce doubled haploid individuals for plant breeding in a way cheaper and faster than through conventional crosses. This technique has been studied in many species and it depends on several factors, such as genotype, culture and donor plant conditions, e...
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| Tipo de recurso: | tesis de maestría |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/76153 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/76153 |
| Access Level: | acceso abierto |
| Palabra clave: | Cultivo de microsporas Colza Pimiento TSA Dobles haploides Lípidos Microspores culture Rape Pepper Doubled haploid Lipids GENETICA Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal |
| Sumario: | [EN] Microspore culture is a useful tool to produce doubled haploid individuals for plant breeding in a way cheaper and faster than through conventional crosses. This technique has been studied in many species and it depends on several factors, such as genotype, culture and donor plant conditions, etc. Efficiency of microspore cultures can be i mproved by modifying the above factors, but also by using compounds that increase such efficiency. One of these compounds is trichostatin A (TSA). It is a histone deacetylase inhibitor, which causes a change in the cell epigenetics and activates some genes . Also causes an increase in embryogenesis efficiency, increasing the number of embryos and dividing cells in culture. In this research, the effects of TSA, in terms of embryogenesis efficiency and at the cellular level, have been evaluated in cultured mic rospores of rapeseed and pepper. For this, rapeseed and pepper microspore cultures were subjected to different treatments (control, DMSO, 0.05 μM TSA and 0.5 μM TSA). Cultured microspores were processed and sectioned for observation at the light microscope , stained with four different dyes: toluidine blue, coomassie blue, iodine - potassium iodide and Sudan Black. |
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