Evaluación de la competencia ginogénica de tres genotipos de cebolla (Allium cepa)

Obtaining double haploid organisms is one of the main objectives of many agricultural companies as well as companies of genetic improvement. The cross between two pure lines allows getting hybrid, homozygous for all its characters. This type of organism presents some agronomic traits of great value,...

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Detalles Bibliográficos
Autor: García Fortea, Edgar
Tipo de recurso: tesis de maestría
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:español
OAI Identifier:oai:riunet.upv.es:10251/86331
Acceso en línea:https://riunet.upv.es/handle/10251/86331
Access Level:acceso abierto
Palabra clave:Embryogenesis
Gynogenesis
Double haploid
Greenhouse
Field
Amiprophos-metyl
Embriogénesis
Ginogésis
Doble haploide
Amiprophos-metil
Allium cepa.
Invernadero
Campo
GENETICA
Máster Universitario en Mejora Genética Vegetal-Màster Universitari en Millora Genètica Vegetal
Descripción
Sumario:Obtaining double haploid organisms is one of the main objectives of many agricultural companies as well as companies of genetic improvement. The cross between two pure lines allows getting hybrid, homozygous for all its characters. This type of organism presents some agronomic traits of great value, as for example hybrid vigor, greater resistance to different biotic and abiotic stress, homogeneity in crops... as well as some genetic features of great interest at the time of getting genetic maps and other studies. Ginogenesis is an alternative method to androgenesis and interspecific hybridization in those species in which these methods do not work to obtain doubled haploids. It presents obvious advantages over the traditional methods, enabling you to get these lines in less time and with fewer economic resources. This work evaluates the gynogenic competence of three commercial onion genotypes (Allium cepa) by adapting the induction protocol of Michalik et al. (2000) for the agroclimatic conditions of the Vera orchard. The aim is verify if is possible to obtain gynogenic embryos and, if it is successful, try to obtain double haploid lines of these genotypes.