TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity

Endothelial progenitor cells (EPCs) have an important role in the maintenance of vascular integrity and homeostasis. While there are many studies that explain EPCs mechanisms action, there are few studies that demonstrate how they interact with other emerging physiological elements such as Endotheli...

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Detalhes bibliográficos
Autores: Luna, Carlos, Carmona, Andrés, Alique, Matilde, Carracedo Añón, Julia María, Ramírez, Rafael
Formato: artículo
Fecha de publicación:2015
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/131422
Acesso em linha:https://hdl.handle.net/20.500.14352/131422
Access Level:acceso abierto
Palavra-chave:612.1
616.1
616.15
576
Endothelial microparticles
Endothelial progenitor cells
Angiogenesis
TNFalpha
HUVECs
Fisiología
Sistema cardiovascular
Hematología
Biología celular (Biología)
2411.03 Fisiología Cardiovascular
3207.04 Patología Cardiovascular
3207.08 Hematología
2410.03 Citología Humana
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oai_identifier_str oai:docta.ucm.es:20.500.14352/131422
network_acronym_str ES
network_name_str España
repository_id_str
spelling TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional ActivityLuna, CarlosCarmona, AndrésAlique, MatildeCarracedo Añón, Julia MaríaRamírez, Rafael612.1616.1616.15576Endothelial microparticlesEndothelial progenitor cellsAngiogenesisTNFalphaHUVECsFisiologíaSistema cardiovascularHematologíaBiología celular (Biología)2411.03 Fisiología Cardiovascular3207.04 Patología Cardiovascular3207.08 Hematología2410.03 Citología HumanaEndothelial progenitor cells (EPCs) have an important role in the maintenance of vascular integrity and homeostasis. While there are many studies that explain EPCs mechanisms action, there are few studies that demonstrate how they interact with other emerging physiological elements such as Endothelial Microparticles (EMPs). EMPs are membranous structures with a size between 100 and 1000 nm that act as molecular information transporter in biological systems and are known as an important elements in develop different pathologies; moreover a lot of works explains that are novel biomarkers. To elucidate these interactions, we proposed an in vitro model of endothelial damage mediated by TNFalpha, in which damaged EMPs and EPCs are in contact to assess EPCs functional effects. We have observed that damaged EMPs can modulate several EPCs classic factors as colony forming units (CFUs), contribution to repair a physically damaged endothelium (wound healing), binding to mature endothelium, and co-adjuvants to the formation of new vessels in vitro (angiogenesis). All of these in a dose-dependent manner. Damaged EMPs at a concentration of 103 MPs/ml have an activating effect of these capabilities, while at concentrations of 105 MPs/ml these effects are attenuated or reduced. This in vitro model helps explain that in diseases where there is an imbalance between these two elements (EPCs and damaged EMPs), the key cellular elements in the regeneration and maintenance of vascular homeostasis (EPCs) are not fully functional, and could explain, at least in part, endothelial dysfunction associated in various pathologies.Frontiers MediaUniversidad Complutense de Madrid20152015-12-2220152015-12-22journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/131422reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not availableInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not availableInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not available Papel de microRNAs vehiculados por micropartículas de células senescentes en la enfermedad vascular asociada a la enfermedad renal crónica por nefropatía diabéticaInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not availableJunta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available JA0797-2010Junta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available P010-CTS-6337Junta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available P11-CTS-7352open accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1314222026-06-02T12:44:21Z
dc.title.none.fl_str_mv TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
title TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
spellingShingle TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
Luna, Carlos
612.1
616.1
616.15
576
Endothelial microparticles
Endothelial progenitor cells
Angiogenesis
TNFalpha
HUVECs
Fisiología
Sistema cardiovascular
Hematología
Biología celular (Biología)
2411.03 Fisiología Cardiovascular
3207.04 Patología Cardiovascular
3207.08 Hematología
2410.03 Citología Humana
title_short TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
title_full TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
title_fullStr TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
title_full_unstemmed TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
title_sort TNFα-Damaged-HUVECs Microparticles Modify Endothelial Progenitor Cell Functional Activity
dc.creator.none.fl_str_mv Luna, Carlos
Carmona, Andrés
Alique, Matilde
Carracedo Añón, Julia María
Ramírez, Rafael
author Luna, Carlos
author_facet Luna, Carlos
Carmona, Andrés
Alique, Matilde
Carracedo Añón, Julia María
Ramírez, Rafael
author_role author
author2 Carmona, Andrés
Alique, Matilde
Carracedo Añón, Julia María
Ramírez, Rafael
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 612.1
616.1
616.15
576
Endothelial microparticles
Endothelial progenitor cells
Angiogenesis
TNFalpha
HUVECs
Fisiología
Sistema cardiovascular
Hematología
Biología celular (Biología)
2411.03 Fisiología Cardiovascular
3207.04 Patología Cardiovascular
3207.08 Hematología
2410.03 Citología Humana
topic 612.1
616.1
616.15
576
Endothelial microparticles
Endothelial progenitor cells
Angiogenesis
TNFalpha
HUVECs
Fisiología
Sistema cardiovascular
Hematología
Biología celular (Biología)
2411.03 Fisiología Cardiovascular
3207.04 Patología Cardiovascular
3207.08 Hematología
2410.03 Citología Humana
description Endothelial progenitor cells (EPCs) have an important role in the maintenance of vascular integrity and homeostasis. While there are many studies that explain EPCs mechanisms action, there are few studies that demonstrate how they interact with other emerging physiological elements such as Endothelial Microparticles (EMPs). EMPs are membranous structures with a size between 100 and 1000 nm that act as molecular information transporter in biological systems and are known as an important elements in develop different pathologies; moreover a lot of works explains that are novel biomarkers. To elucidate these interactions, we proposed an in vitro model of endothelial damage mediated by TNFalpha, in which damaged EMPs and EPCs are in contact to assess EPCs functional effects. We have observed that damaged EMPs can modulate several EPCs classic factors as colony forming units (CFUs), contribution to repair a physically damaged endothelium (wound healing), binding to mature endothelium, and co-adjuvants to the formation of new vessels in vitro (angiogenesis). All of these in a dose-dependent manner. Damaged EMPs at a concentration of 103 MPs/ml have an activating effect of these capabilities, while at concentrations of 105 MPs/ml these effects are attenuated or reduced. This in vitro model helps explain that in diseases where there is an imbalance between these two elements (EPCs and damaged EMPs), the key cellular elements in the regeneration and maintenance of vascular homeostasis (EPCs) are not fully functional, and could explain, at least in part, endothelial dysfunction associated in various pathologies.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-12-22
2015
2015-12-22
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/131422
url https://hdl.handle.net/20.500.14352/131422
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not available
Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not available
Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not available Papel de microRNAs vehiculados por micropartículas de células senescentes en la enfermedad vascular asociada a la enfermedad renal crónica por nefropatía diabética
Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 EC Not available
Junta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available JA0797-2010
Junta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available P010-CTS-6337
Junta de Andalucía http://dx.doi.org/10.13039/501100011011 Not available P11-CTS-7352
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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