Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy

Background: Ultrasound is considered a safe and non-invasive tool in regenerative medicine. In particular, low-intensity pulsed ultrasound (LIPUS) has been used in the clinic for more than twenty years for applications in bone healing. It has been demonstrated to be an effective tool to treat differ...

Descripción completa

Detalles Bibliográficos
Autores: González Gálvez, Beatriz, Fernández Marcelo, Tamara, Calero, Alba, Sury, Paulina, Lucas, Beatriz de
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/121470
Acceso en línea:https://hdl.handle.net/20.500.14352/121470
Access Level:acceso abierto
Palabra clave:616-006.64-092.18
577.2
616-006.04
Low-intensity pulsed ultrasound
Breast cancer stem cell
Migration
Invasion
Angiogenesis
Ciencias Biomédicas
Biología molecular (Biología)
Oncología
32 Ciencias Médicas
2407 Biología Celular
3207.13 Oncología
id ES_e1ea2160710bd87d3ee2705ec0a3db53
oai_identifier_str oai:docta.ucm.es:20.500.14352/121470
network_acronym_str ES
network_name_str España
repository_id_str
spelling Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapyGonzález Gálvez, BeatrizFernández Marcelo, TamaraCalero, AlbaSury, PaulinaLucas, Beatriz de616-006.64-092.18577.2616-006.04Low-intensity pulsed ultrasoundBreast cancer stem cellMigrationInvasionAngiogenesisCiencias BiomédicasBiología molecular (Biología)Oncología32 Ciencias Médicas2407 Biología Celular3207.13 OncologíaBackground: Ultrasound is considered a safe and non-invasive tool in regenerative medicine. In particular, low-intensity pulsed ultrasound (LIPUS) has been used in the clinic for more than twenty years for applications in bone healing. It has been demonstrated to be an effective tool to treat different chronic diseases. We sought to evaluate the effects produced by LIPUS on the properties of human breast cancer stem cells (bCSCs). Methods: Cells were stimulated using a traditional ultrasound device with the following parameters: 0.05 W/cm2 with 10% duty cycle, frequency of 3 MHz and 8 pulses. Results: At the parameters used, the ultrasound did not directly affect bCSC proliferation, with no evident changes in morphology. In contrast, the ultrasound protocol affected the migration and invasion ability of bCSCs, limiting their capacity to advance while a major affection was detected on their angiogenic properties. LIPUS-treated bCSCs were unable to transform into aggressive metastatic cancer cells, by decreasing their migration and invasion capacity as well as vessel formation. Finally, RNA-seq analysis revealed major changes in gene expression, with 676 differentially expressed genes after LIPUS stimulation, 578 upregulated and 98 downregulated. Conclusions: Overall, these results highlight the potential of LIPUS as a promising non-invasive therapy to target bCSCs and attenuate its capacity to drive migration, invasion, angiogenesis and, ultimately, tumor malignancy. Besides, the ability of LIPUS to modulate gene expression points out its capacity to broadly influence the cellular transcriptome. Therefore, the application of LIPUS as an antitumor therapeutic agent targeting bCSCs may offer a promising new approach to treat cancer. In vivo functional experiments will determine in the future the relevance of LIPUS application for the development of metastasis.BioMed Central (BMC)Universidad Complutense de Madrid20252025-01-0120252025-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/121470reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Not available CNS2022-135241open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1214702026-06-02T12:44:21Z
dc.title.none.fl_str_mv Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
title Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
spellingShingle Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
González Gálvez, Beatriz
616-006.64-092.18
577.2
616-006.04
Low-intensity pulsed ultrasound
Breast cancer stem cell
Migration
Invasion
Angiogenesis
Ciencias Biomédicas
Biología molecular (Biología)
Oncología
32 Ciencias Médicas
2407 Biología Celular
3207.13 Oncología
title_short Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
title_full Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
title_fullStr Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
title_full_unstemmed Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
title_sort Migration and invasion of cancer stem cells are prevented by low-intensity pulsed ultrasound therapy
dc.creator.none.fl_str_mv González Gálvez, Beatriz
Fernández Marcelo, Tamara
Calero, Alba
Sury, Paulina
Lucas, Beatriz de
author González Gálvez, Beatriz
author_facet González Gálvez, Beatriz
Fernández Marcelo, Tamara
Calero, Alba
Sury, Paulina
Lucas, Beatriz de
author_role author
author2 Fernández Marcelo, Tamara
Calero, Alba
Sury, Paulina
Lucas, Beatriz de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616-006.64-092.18
577.2
616-006.04
Low-intensity pulsed ultrasound
Breast cancer stem cell
Migration
Invasion
Angiogenesis
Ciencias Biomédicas
Biología molecular (Biología)
Oncología
32 Ciencias Médicas
2407 Biología Celular
3207.13 Oncología
topic 616-006.64-092.18
577.2
616-006.04
Low-intensity pulsed ultrasound
Breast cancer stem cell
Migration
Invasion
Angiogenesis
Ciencias Biomédicas
Biología molecular (Biología)
Oncología
32 Ciencias Médicas
2407 Biología Celular
3207.13 Oncología
description Background: Ultrasound is considered a safe and non-invasive tool in regenerative medicine. In particular, low-intensity pulsed ultrasound (LIPUS) has been used in the clinic for more than twenty years for applications in bone healing. It has been demonstrated to be an effective tool to treat different chronic diseases. We sought to evaluate the effects produced by LIPUS on the properties of human breast cancer stem cells (bCSCs). Methods: Cells were stimulated using a traditional ultrasound device with the following parameters: 0.05 W/cm2 with 10% duty cycle, frequency of 3 MHz and 8 pulses. Results: At the parameters used, the ultrasound did not directly affect bCSC proliferation, with no evident changes in morphology. In contrast, the ultrasound protocol affected the migration and invasion ability of bCSCs, limiting their capacity to advance while a major affection was detected on their angiogenic properties. LIPUS-treated bCSCs were unable to transform into aggressive metastatic cancer cells, by decreasing their migration and invasion capacity as well as vessel formation. Finally, RNA-seq analysis revealed major changes in gene expression, with 676 differentially expressed genes after LIPUS stimulation, 578 upregulated and 98 downregulated. Conclusions: Overall, these results highlight the potential of LIPUS as a promising non-invasive therapy to target bCSCs and attenuate its capacity to drive migration, invasion, angiogenesis and, ultimately, tumor malignancy. Besides, the ability of LIPUS to modulate gene expression points out its capacity to broadly influence the cellular transcriptome. Therefore, the application of LIPUS as an antitumor therapeutic agent targeting bCSCs may offer a promising new approach to treat cancer. In vivo functional experiments will determine in the future the relevance of LIPUS application for the development of metastasis.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-01-01
2025
2025-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/121470
url https://hdl.handle.net/20.500.14352/121470
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Not available CNS2022-135241
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422337827274752
score 15,812429

Similares en LA Referencia