Multiphoton imaging microscopy at deeper layers with adaptive optics control of spherical aberration
Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universitat Politècnica de Catalunya (UPC) |
| Repositorio: | UPCommons. Portal del coneixement obert de la UPC |
| Idioma: | inglés |
| OAI Identifier: | oai:upcommons.upc.edu:2117/346017 |
| Acceso en línea: | https://hdl.handle.net/2117/346017 https://dx.doi.org/10.1117/1.jbo.19.1.011007 |
| Access Level: | acceso abierto |
| Palabra clave: | Multiphoton processes Fluorescence microscopy Multiphoton microscopy Wavefront aberration Adaptive optics Human ocular tissue Microscòpia de fluorescència Àrees temàtiques de la UPC::Física::Física de partícules::Fotons |
| Sumario: | Despite the inherent confocality and optical sectioning capabilities of multiphoton microscopy, threedimensional (3-D) imaging of thick samples is limited by the specimen-induced aberrations. The combination of immersion objectives and sensorless adaptive optics (AO) techniques has been suggested to overcome this difficulty. However, a complex plane-by-plane correction of aberrations is required, and its performance depends on a set of image-based merit functions. We propose here an alternative approach to increase penetration depth in 3-D multiphoton microscopy imaging. |
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