Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model

Background In a previous study, the 84-day administration of glycosaminoglycans (GAGs), with or without native collagen type II (NC), in an osteoarthritis (OA)-induced rabbit model slowed down OA progression, improved several micro- and macroscopic parameters and magnetic resonance imaging (MRI) bio...

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Autores: Mariné-Casadó, Roger, Domenech-Coca, Cristina, Fernández, Salvador, Costa, Andrea, Segarra, Sergi, López-Andreo, José, Puiggròs, Francesc, Cerón, José Joaquín, Martínez-Puig, Daniel, Soler i Canet, María del Carme, Sifre Canet, Vicente José, Serra Aguado, Claudio Iván, Caimari, Antoni
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/5014
Acceso en línea:http://hdl.handle.net/20.500.12466/5014
Access Level:acceso abierto
Palabra clave:Transcriptomics
Osteoarthritis
Articular cartilage
Native type II collagen
Nutraceuticals
2401.08 Genética Animal
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spelling Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit modelMariné-Casadó, RogerDomenech-Coca, CristinaFernández, SalvadorCosta, AndreaSegarra, SergiLópez-Andreo, JoséPuiggròs, FrancescCerón, José JoaquínMartínez-Puig, DanielSoler i Canet, María del CarmeSifre Canet, Vicente JoséSerra Aguado, Claudio IvánCaimari, AntoniTranscriptomicsOsteoarthritisArticular cartilageNative type II collagenNutraceuticals2401.08 Genética AnimalBackground In a previous study, the 84-day administration of glycosaminoglycans (GAGs), with or without native collagen type II (NC), in an osteoarthritis (OA)-induced rabbit model slowed down OA progression, improved several micro- and macroscopic parameters and magnetic resonance imaging (MRI) biomarkers in cartilage, and increased hyaluronic acid levels in synovial fluid. To elucidate the potential underlying mechanisms, a transcriptomics approach was conducted using medial femoral condyle and trochlea samples. Results The administration of chondroitin sulfate (CS), glucosamine hydrochloride (GlHCl), and hyaluronic acid (HA), with (CGH-NC) or without (CGH) NC, strongly modulated several genes involved in chondrocyte extracellular matrix (ECM) remodeling and homeostasis when compared to non-treated rabbits (CTR group). Notably, both treatments shared the main mechanism of action, which was related to ECM modulation through the down-regulation of genes encoding proteolytic enzymes, such as ADAM metallopeptidase with thrombospondin type 1 motif, 9 (Adamts9), and the overexpression of genes with a relevant role in the synthesis of ECM components, such as aggrecan (Acan) in both CGH-NC and CGH groups, and fibronectin 1 (Fn1) and collagen type II, alpha 1 (Col2A1) in the CGH group. Furthermore, there was a significant modulation at the gene expression level of the mTOR signaling pathway, which is associated with the regulation of the synthesis of ECM proteolytic enzymes, only in CGH-NC-supplemented rabbits. This modulation could account for the better outcomes concerning the microscopic and macroscopic evaluations reported in these animals.Conclusions In conclusion, the expression of key genes involved in chondrocyte ECM remodeling and homeostasis was significantly modulated in rabbits in response to both CGH and CGH-NC treatments, which would partly explain the mechanisms by which these therapies exert beneficial effects against OA.20252025-01-0820242024-09-0420242024-09-04journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/5014reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/50142026-06-19T08:32:07Z
dc.title.none.fl_str_mv Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
title Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
spellingShingle Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
Mariné-Casadó, Roger
Transcriptomics
Osteoarthritis
Articular cartilage
Native type II collagen
Nutraceuticals
2401.08 Genética Animal
title_short Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
title_full Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
title_fullStr Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
title_full_unstemmed Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
title_sort Effects of the oral administration of glycosaminoglycans with or without native type II collagen on the articular cartilage transcriptome in an osteoarthritic-induced rabbit model
dc.creator.none.fl_str_mv Mariné-Casadó, Roger
Domenech-Coca, Cristina
Fernández, Salvador
Costa, Andrea
Segarra, Sergi
López-Andreo, José
Puiggròs, Francesc
Cerón, José Joaquín
Martínez-Puig, Daniel
Soler i Canet, María del Carme
Sifre Canet, Vicente José
Serra Aguado, Claudio Iván
Caimari, Antoni
author Mariné-Casadó, Roger
author_facet Mariné-Casadó, Roger
Domenech-Coca, Cristina
Fernández, Salvador
Costa, Andrea
Segarra, Sergi
López-Andreo, José
Puiggròs, Francesc
Cerón, José Joaquín
Martínez-Puig, Daniel
Soler i Canet, María del Carme
Sifre Canet, Vicente José
Serra Aguado, Claudio Iván
Caimari, Antoni
author_role author
author2 Domenech-Coca, Cristina
Fernández, Salvador
Costa, Andrea
Segarra, Sergi
López-Andreo, José
Puiggròs, Francesc
Cerón, José Joaquín
Martínez-Puig, Daniel
Soler i Canet, María del Carme
Sifre Canet, Vicente José
Serra Aguado, Claudio Iván
Caimari, Antoni
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Transcriptomics
Osteoarthritis
Articular cartilage
Native type II collagen
Nutraceuticals
2401.08 Genética Animal
topic Transcriptomics
Osteoarthritis
Articular cartilage
Native type II collagen
Nutraceuticals
2401.08 Genética Animal
description Background In a previous study, the 84-day administration of glycosaminoglycans (GAGs), with or without native collagen type II (NC), in an osteoarthritis (OA)-induced rabbit model slowed down OA progression, improved several micro- and macroscopic parameters and magnetic resonance imaging (MRI) biomarkers in cartilage, and increased hyaluronic acid levels in synovial fluid. To elucidate the potential underlying mechanisms, a transcriptomics approach was conducted using medial femoral condyle and trochlea samples. Results The administration of chondroitin sulfate (CS), glucosamine hydrochloride (GlHCl), and hyaluronic acid (HA), with (CGH-NC) or without (CGH) NC, strongly modulated several genes involved in chondrocyte extracellular matrix (ECM) remodeling and homeostasis when compared to non-treated rabbits (CTR group). Notably, both treatments shared the main mechanism of action, which was related to ECM modulation through the down-regulation of genes encoding proteolytic enzymes, such as ADAM metallopeptidase with thrombospondin type 1 motif, 9 (Adamts9), and the overexpression of genes with a relevant role in the synthesis of ECM components, such as aggrecan (Acan) in both CGH-NC and CGH groups, and fibronectin 1 (Fn1) and collagen type II, alpha 1 (Col2A1) in the CGH group. Furthermore, there was a significant modulation at the gene expression level of the mTOR signaling pathway, which is associated with the regulation of the synthesis of ECM proteolytic enzymes, only in CGH-NC-supplemented rabbits. This modulation could account for the better outcomes concerning the microscopic and macroscopic evaluations reported in these animals.Conclusions In conclusion, the expression of key genes involved in chondrocyte ECM remodeling and homeostasis was significantly modulated in rabbits in response to both CGH and CGH-NC treatments, which would partly explain the mechanisms by which these therapies exert beneficial effects against OA.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-09-04
2024
2024-09-04
2025
2025-01-08
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/5014
url http://hdl.handle.net/20.500.12466/5014
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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