Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels

9 pages, 4 figures, 1 table

Detalles Bibliográficos
Autores: López-Sánchez, Patricia, Schuster, Erich, Wang, Dongjie, Gidley, Michael J., Strom, Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/377219
Acceso en línea:http://hdl.handle.net/10261/377219
Access Level:acceso abierto
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spelling Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogelsLópez-Sánchez, PatriciaSchuster, ErichWang, DongjieGidley, Michael J.Strom, Anna9 pages, 4 figures, 1 tableCellulose hydrogels are extensively applied in many biotechnological fields and are also used as models for plant cell walls. We synthesised model cellulosic hydrogels containing hemicelluloses, as a biomimetic of plant cell walls, in order to study the role of hemicelluloses on their mass transport properties. Microbial cellulose is able to self-assemble into composites when hemicelluloses, such as xyloglucan and arabinoxylan, are present in the incubation media, leading to hydrogels with different nano and microstructures. We investigated the diffusivities of a series of fluorescently labelled dextrans, of different molecular weight, and proteins, including a plant pectin methyl esterase (PME), using fluorescence recovery after photobleaching (FRAP). The presence of xyloglucan, known to be able to crosslink cellulose fibres, confirmed by scanning electron microscopy (SEM) and 13C NMR, reduced mobility of macromolecules of molecular weight higher than 10 kDa, reflected in lower diffusion coefficients. Furthermore PME diffusion was reduced in composites containing xyloglucan, despite the lack of a particular binding motif in PME for this polysaccharide, suggesting possible non-specific interactions between PME and this hemicellulose. In contrast, hydrogels containing arabinoxylan coating cellulose fibres showed enhanced diffusivity of the molecules studied. The different diffusivities were related to the architectural features found in the composites as a function of polysaccharide composition. Our results show the effect of model hemicelluloses in the mass transport properties of cellulose networks in highly hydrated environments relevant to understanding the role of hemicelluloses in the permeability of plant cell walls and aiding design of plant based materials with tailored propertiesThe financial support from VINN EXcellence SuMo Biomaterials (Supramolecular Biomaterials – Structure dynamics and properties) to AS and ES as well as VINNMER to AS are acknowledged. This work was carried out with the financial support of a UQ Start-up research grant to PLSPeer reviewedRoyal Society of Chemistry (UK)202520252015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/377219reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1039/C5SM00103JNoinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3772192026-05-22T06:33:51Z
dc.title.none.fl_str_mv Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
title Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
spellingShingle Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
López-Sánchez, Patricia
title_short Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
title_full Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
title_fullStr Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
title_full_unstemmed Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
title_sort Diffusion of macromolecules in self-assembled cellulose/hemicellulose hydrogels
dc.creator.none.fl_str_mv López-Sánchez, Patricia
Schuster, Erich
Wang, Dongjie
Gidley, Michael J.
Strom, Anna
author López-Sánchez, Patricia
author_facet López-Sánchez, Patricia
Schuster, Erich
Wang, Dongjie
Gidley, Michael J.
Strom, Anna
author_role author
author2 Schuster, Erich
Wang, Dongjie
Gidley, Michael J.
Strom, Anna
author2_role author
author
author
author
description 9 pages, 4 figures, 1 table
publishDate 2015
dc.date.none.fl_str_mv 2015
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/377219
url http://hdl.handle.net/10261/377219
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1039/C5SM00103J
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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