Comparative transcriptomics of host-pathogen interactions and hybridization in Candida pathogens
Candida pathogenic yeasts represent a global healthcare problem. They comprise phylogenetically diverse species, including newly emerged pathogens. How human-Candida interactions vary across species, and what processes underlie the emergence of novel pathogens are poorly understood. Current thesis a...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/670316 |
| Acceso en línea: | http://hdl.handle.net/10803/670316 |
| Access Level: | acceso abierto |
| Palabra clave: | Candida Host-pathogens interactions Comparative transcriptomics lncRNAs Hybridization Interacciones huésped-patógeno Transcriptómica comparativa Hibridación 577 |
| Sumario: | Candida pathogenic yeasts represent a global healthcare problem. They comprise phylogenetically diverse species, including newly emerged pathogens. How human-Candida interactions vary across species, and what processes underlie the emergence of novel pathogens are poorly understood. Current thesis addresses these issues using comparative transcriptomics and bioinformatics. We established the global patterns of host-pathogen interactions between human host and the main Candida species, providing novel mechanistic insights into their interplay. We also explored lncRNAs of these pathogens, assessing their implications in infection. Further, we designed and validated a pan-Candida RNA enrichment approach, opening new possibilities for studying host-pathogen interactions in vivo. Then, we assessed the impact of hybridization on transcriptomes of hybrid yeasts, exploring the links between hybridization and virulence emergence. We also developed a new bioinformatics tool facilitating the research in the field. Altogether, results of this thesis expand our knowledge on relevant aspects of human-Candida interactions and yeast evolution. |
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