Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis
The interplay between T cells, dendritic cells and keratinocytes is crucial for the development and maintenance of inflammation in psoriasis. GADD45 proteins mediate DNA repair in different cells including keratinocytes. In the immune system, GADD45a and GADD45b regulate the function and activation...
| Autores: | , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/97235 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/97235 |
| Access Level: | acceso abierto |
| Palabra clave: | 612.017 GADD45 proteins Psoriasis Immune responses Biología molecular (Biología) Biología celular (Biología) Bioquímica (Biología) Inmunología 2407 Biología Celular 2412 Inmunología 2403 Bioquímica 2415 Biología Molecular |
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Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasisRodríguez-Jiménez, PedroFernández Messina, Lola MaríaOvejero-Benito, MaríaChicharro, PabloVera-Tomé, PaulaVara, AliciaCibrian, DanayMartínez-Fleta, PedroJiménez-Fernández, MaríaSánchez-García, InésLlamas-Velasco, MarAbad Santos, FranciscoSánchez-Madrid, FranciscoDauden, EstebanFuente, Hortensia de la612.017GADD45 proteinsPsoriasisImmune responsesBiología molecular (Biología)Biología celular (Biología)Bioquímica (Biología)Inmunología2407 Biología Celular2412 Inmunología2403 Bioquímica2415 Biología MolecularThe interplay between T cells, dendritic cells and keratinocytes is crucial for the development and maintenance of inflammation in psoriasis. GADD45 proteins mediate DNA repair in different cells including keratinocytes. In the immune system, GADD45a and GADD45b regulate the function and activation of both T lymphocytes and dendritic cells and GADD45a links DNA repair and epigenetic regulation through its demethylase activity. Here, we analyzed the expression of GADD45a and GADD45b in the skin, dendritic cells and circulating T cells in a cohort of psoriasis patients and their regulation by inflammatory signals. Thirty patients (17 male/13 female) with plaque psoriasis and 15 controls subjects (7 male/8 female), were enrolled. Psoriasis patients exhibited a lower expression of GADD45a at the epidermis but a higher expression in dermal infiltrating T cells in lesional skin. The expression of GADD45a and GADD45b was also higher in peripheral T cells from psoriasis patients, although no differences were observed in p38 activation. The expression and methylation state of the GADD45a target UCHL1 were evaluated, revealing a hypermethylation of its promoter in lesional skin compared to controls. Furthermore, reduced levels of GADD45a correlated with a lower expression UCHL1 in lesional skin. We propose that the demethylase function of GADD45a may account for its pleiotropic effects, and the complex and heterogeneous pattern of expression observed in psoriatic disease.Nature ResearchUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/97235reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/972352026-06-02T12:44:21Z |
| dc.title.none.fl_str_mv |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| title |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| spellingShingle |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis Rodríguez-Jiménez, Pedro 612.017 GADD45 proteins Psoriasis Immune responses Biología molecular (Biología) Biología celular (Biología) Bioquímica (Biología) Inmunología 2407 Biología Celular 2412 Inmunología 2403 Bioquímica 2415 Biología Molecular |
| title_short |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| title_full |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| title_fullStr |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| title_full_unstemmed |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| title_sort |
Growth arrest and DNA damage-inducible proteins (GADD45) in psoriasis |
| dc.creator.none.fl_str_mv |
Rodríguez-Jiménez, Pedro Fernández Messina, Lola María Ovejero-Benito, María Chicharro, Pablo Vera-Tomé, Paula Vara, Alicia Cibrian, Danay Martínez-Fleta, Pedro Jiménez-Fernández, María Sánchez-García, Inés Llamas-Velasco, Mar Abad Santos, Francisco Sánchez-Madrid, Francisco Dauden, Esteban Fuente, Hortensia de la |
| author |
Rodríguez-Jiménez, Pedro |
| author_facet |
Rodríguez-Jiménez, Pedro Fernández Messina, Lola María Ovejero-Benito, María Chicharro, Pablo Vera-Tomé, Paula Vara, Alicia Cibrian, Danay Martínez-Fleta, Pedro Jiménez-Fernández, María Sánchez-García, Inés Llamas-Velasco, Mar Abad Santos, Francisco Sánchez-Madrid, Francisco Dauden, Esteban Fuente, Hortensia de la |
| author_role |
author |
| author2 |
Fernández Messina, Lola María Ovejero-Benito, María Chicharro, Pablo Vera-Tomé, Paula Vara, Alicia Cibrian, Danay Martínez-Fleta, Pedro Jiménez-Fernández, María Sánchez-García, Inés Llamas-Velasco, Mar Abad Santos, Francisco Sánchez-Madrid, Francisco Dauden, Esteban Fuente, Hortensia de la |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad Complutense de Madrid |
| dc.subject.none.fl_str_mv |
612.017 GADD45 proteins Psoriasis Immune responses Biología molecular (Biología) Biología celular (Biología) Bioquímica (Biología) Inmunología 2407 Biología Celular 2412 Inmunología 2403 Bioquímica 2415 Biología Molecular |
| topic |
612.017 GADD45 proteins Psoriasis Immune responses Biología molecular (Biología) Biología celular (Biología) Bioquímica (Biología) Inmunología 2407 Biología Celular 2412 Inmunología 2403 Bioquímica 2415 Biología Molecular |
| description |
The interplay between T cells, dendritic cells and keratinocytes is crucial for the development and maintenance of inflammation in psoriasis. GADD45 proteins mediate DNA repair in different cells including keratinocytes. In the immune system, GADD45a and GADD45b regulate the function and activation of both T lymphocytes and dendritic cells and GADD45a links DNA repair and epigenetic regulation through its demethylase activity. Here, we analyzed the expression of GADD45a and GADD45b in the skin, dendritic cells and circulating T cells in a cohort of psoriasis patients and their regulation by inflammatory signals. Thirty patients (17 male/13 female) with plaque psoriasis and 15 controls subjects (7 male/8 female), were enrolled. Psoriasis patients exhibited a lower expression of GADD45a at the epidermis but a higher expression in dermal infiltrating T cells in lesional skin. The expression of GADD45a and GADD45b was also higher in peripheral T cells from psoriasis patients, although no differences were observed in p38 activation. The expression and methylation state of the GADD45a target UCHL1 were evaluated, revealing a hypermethylation of its promoter in lesional skin compared to controls. Furthermore, reduced levels of GADD45a correlated with a lower expression UCHL1 in lesional skin. We propose that the demethylase function of GADD45a may account for its pleiotropic effects, and the complex and heterogeneous pattern of expression observed in psoriatic disease. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/20.500.14352/97235 |
| url |
https://hdl.handle.net/20.500.14352/97235 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Nature Research |
| publisher.none.fl_str_mv |
Nature Research |
| dc.source.none.fl_str_mv |
reponame:Docta Complutense instname:Universidad Complutense de Madrid (UCM) |
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Universidad Complutense de Madrid (UCM) |
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Docta Complutense |
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Docta Complutense |
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