MiRNA post-transcriptional modification dynamics in T cell activation
T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modi...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | inglés |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/97230 |
| Acceso en línea: | https://hdl.handle.net/20.500.14352/97230 |
| Access Level: | acceso abierto |
| Palabra clave: | 612.017 microRNAs T cells Immune activation RNA modifying enzymes microRNA post-transcriptonal modification Biología celular (Biología) Biología molecular (Biología) Bioquímica (Biología) Inmunología 2407 Biología Celular 2403 Bioquímica 2415 Biología Molecular 2412 Inmunología |
| Sumario: | T cell activation leads to extensive changes in the miRNA repertoire. Although overall miRNA expression decreases within a few hours of T cell activation, some individual miRNAs are specifically upregulated. Using next-generation sequencing, we assessed miRNA expression and post-transcriptional modification kinetics in human primary CD4+ T cells upon T cell receptor (TCR) or type I interferon stimulation. This analysis identified differential expression of multiple miRNAs not previously linked to T cell activation. Remarkably, upregulated miRNAs showed a higher frequency of 3′ adenylation. TCR stimulation was followed by increased expression of RNA modifying enzymes and the RNA degrading enzymes Dis3L2 and Eri1. In the midst of this adverse environment, 3′ adenylation may serve a protective function that could be exploited to improve miRNA stability for T cell-targeted therapy. |
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