Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens

7 páginas, 4 figuras.

Detalles Bibliográficos
Autores: Diosdado Martín, Alicia, Simón Marta, Fernando, Morchón, Rodrigo, González Miguel, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/227532
Acceso en línea:http://hdl.handle.net/10261/227532
Access Level:acceso abierto
Palabra clave:Anticoagulants
Coagulation cascade
Dirofilaria immitis
Factor Xa
Excretory/secretory antigens
Serpin
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spelling Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigensDiosdado Martín, AliciaSimón Marta, FernandoMorchón, RodrigoGonzález Miguel, JavierAnticoagulantsCoagulation cascadeDirofilaria immitisFactor XaExcretory/secretory antigensSerpin7 páginas, 4 figuras.Dirofilaria immitis is a parasitic nematode that survives in the circulatory system of suitable hosts for many years, causing the most severe thromboembolisms when simultaneous death of adult worms occurs. The two main mechanisms responsible for thrombus formation in mammals are the activation and aggregation of platelets and the generation of fibrin through the coagulation cascade. The aim of this work was to study the anticoagulant potential of excretory/secretory antigens from D. immitis adult worms (DiES) on the coagulation cascade of the host. Anticoagulant and inhibition assays respectively showed that DiES partially alter the coagulation cascade of the host and reduce the activity of the coagulation factor Xa, a key enzyme in the coagulation process. In addition, a D. immitis protein was identified by its similarity to the homologous serpin 6 from Brugia malayi as a possible candidate to form an inhibitory complex with FXa by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectrometry. These results indicate that D. immitis could use the anticoagulant properties of its excretory/secretory antigens to control the formation of blood clots in its immediate intravascular habitat as a survival mechanismJGM is supported by the JIN project ‘ULYSSES’ (RTI2018-093463-J-100) funded by Ministerio de Ciencia, Innovación y Universidades (MCIU), Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER, UE).Peer reviewedCambridge University PressMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European CommissionGonzález Miguel, Javier [0000-0003-4279-4761]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/227532reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093463-J-100https://doi.org/10.1017/S0031182020000104Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2275322026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
title Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
spellingShingle Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
Diosdado Martín, Alicia
Anticoagulants
Coagulation cascade
Dirofilaria immitis
Factor Xa
Excretory/secretory antigens
Serpin
title_short Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
title_full Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
title_fullStr Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
title_full_unstemmed Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
title_sort Dirofilaria immitis possesses molecules with anticoagulant properties in its excretory/secretory antigens
dc.creator.none.fl_str_mv Diosdado Martín, Alicia
Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author Diosdado Martín, Alicia
author_facet Diosdado Martín, Alicia
Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author_role author
author2 Simón Marta, Fernando
Morchón, Rodrigo
González Miguel, Javier
author2_role author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
European Commission
González Miguel, Javier [0000-0003-4279-4761]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anticoagulants
Coagulation cascade
Dirofilaria immitis
Factor Xa
Excretory/secretory antigens
Serpin
topic Anticoagulants
Coagulation cascade
Dirofilaria immitis
Factor Xa
Excretory/secretory antigens
Serpin
description 7 páginas, 4 figuras.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/227532
url http://hdl.handle.net/10261/227532
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093463-J-100
https://doi.org/10.1017/S0031182020000104
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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