In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles

The aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) crosslinked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning...

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Authors: Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099, Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4, Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024a, Quincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80d, Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b, Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353, Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173, Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4
Format: article
Publication Date:2020
Country:España
Institution:Universidad de Navarra
Repository:Dadun. Depósito Académico Digital de la Universidad de Navarra
Language:English
OAI Identifier:oai:dadun.unav.edu:10171/115240
Online Access:https://hdl.handle.net/10171/115240
Access Level:Open access
Keyword:Human serum albumin
Bevacizumab
Nanoparticles
Molecular imaging
SPECT/CT
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spelling In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticlesRamos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024aQuincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80dCollantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626bEcay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4Human serum albuminBevacizumabNanoparticlesMolecular imagingSPECT/CTThe aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) crosslinked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning method ([99mTc]TcNP-Ab). The Ab was labeled using [ 99mTc][Tc(CO)3(H2O)3] + and used to prepare nanoparticles (NP-[99mTc]TcAb). Particle size was similar in both formulations. Chemical and radiochemical purity of the two nanosystems were >95%. Bevacizumab-labeling conditions were tested by in vitro stability studies. More than 87% of the radiolabeled antibody remained intact for 24 h after incubation with plasma. SPECT/CT imaging of the two nanoparticles was performed in healthy female Wistar rats. Ex vivo gamma counting of selected organs was also carried out in all animals. The results showed different clearance rates of the nanoparticle shell and the antibody, providing valuable information by the use of molecular imaging in the evaluation of drug delivery nanosystems.Elsevier ScienceDadun. Depósito Académico Digital Universidad de Navarra20202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articletext/htmlhttps://hdl.handle.net/10171/115240reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/1152402026-06-21T12:47:57Z
dc.title.none.fl_str_mv In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
title In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
spellingShingle In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099
Human serum albumin
Bevacizumab
Nanoparticles
Molecular imaging
SPECT/CT
title_short In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
title_full In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
title_fullStr In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
title_full_unstemmed In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
title_sort In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles
dc.creator.none.fl_str_mv Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099
Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4
Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024a
Quincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80d
Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b
Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4
author Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099
author_facet Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099
Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4
Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024a
Quincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80d
Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b
Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4
author_role author
author2 Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4
Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024a
Quincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80d
Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b
Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353
Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173
Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Human serum albumin
Bevacizumab
Nanoparticles
Molecular imaging
SPECT/CT
topic Human serum albumin
Bevacizumab
Nanoparticles
Molecular imaging
SPECT/CT
description The aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) crosslinked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning method ([99mTc]TcNP-Ab). The Ab was labeled using [ 99mTc][Tc(CO)3(H2O)3] + and used to prepare nanoparticles (NP-[99mTc]TcAb). Particle size was similar in both formulations. Chemical and radiochemical purity of the two nanosystems were >95%. Bevacizumab-labeling conditions were tested by in vitro stability studies. More than 87% of the radiolabeled antibody remained intact for 24 h after incubation with plasma. SPECT/CT imaging of the two nanoparticles was performed in healthy female Wistar rats. Ex vivo gamma counting of selected organs was also carried out in all animals. The results showed different clearance rates of the nanoparticle shell and the antibody, providing valuable information by the use of molecular imaging in the evaluation of drug delivery nanosystems.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/115240
url https://hdl.handle.net/10171/115240
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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