In vivo SPECT-CT imaging and characterization of technetium-99m-labeled bevacizumab-loaded human serum albumin pegylated nanoparticles

The aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) crosslinked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning...

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Detalles Bibliográficos
Autores: Ramos-Membrive, R. (Rocío)|||/items/e6076e57-39f9-4d1d-b490-0378e81cd099, Erhard, Á. (Álvaro)|||/items/fc7d34c9-224d-4d19-b57d-e7f85d11e4b4, Luis-de-Redín-Subirá, I. (Inés)|||/items/54262ec8-a9d7-4359-9947-775a8e95024a, Quincoces-Fernández, G. (Gemma)|||/items/fa93ac37-c637-4dad-be78-c9a892ebb80d, Collantes, M. (María)|||/items/102ad3e7-3f57-4958-8e97-39af1c92626b, Ecay, M. (Margarita)|||/items/62075174-6491-4648-a96e-d724a641d353, Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173, Peñuelas-Sanchez, I. (Ivan)|||/items/7022e2fa-b3b0-48ed-8d39-2020cfae85a4
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/115240
Acceso en línea:https://hdl.handle.net/10171/115240
Access Level:acceso abierto
Palabra clave:Human serum albumin
Bevacizumab
Nanoparticles
Molecular imaging
SPECT/CT
Descripción
Sumario:The aim of this work was to study the biodistribution of bevacizumab-loaded HSA nanoparticles (NP-Ab) crosslinked with PEG35000 by SPECT/CT in vivo imaging. For this purpose, NP-Abs were prepared by a desolvation process, coated with PEG35000 and radiolabeled with technetium-99 m using a pre-tinning method ([99mTc]TcNP-Ab). The Ab was labeled using [ 99mTc][Tc(CO)3(H2O)3] + and used to prepare nanoparticles (NP-[99mTc]TcAb). Particle size was similar in both formulations. Chemical and radiochemical purity of the two nanosystems were >95%. Bevacizumab-labeling conditions were tested by in vitro stability studies. More than 87% of the radiolabeled antibody remained intact for 24 h after incubation with plasma. SPECT/CT imaging of the two nanoparticles was performed in healthy female Wistar rats. Ex vivo gamma counting of selected organs was also carried out in all animals. The results showed different clearance rates of the nanoparticle shell and the antibody, providing valuable information by the use of molecular imaging in the evaluation of drug delivery nanosystems.

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