A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection
In this work we present the preparation of a 2D molybdenum disulphide nanosheets (2D-MoS2) and tetrahedral DNA nanostructures (TDNs) bioconjugate, and its application to the development of a bioassay for rapid and easy virus detection. The bioconjugate has been prepared by using TDNs carrying the ca...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/711010 |
| Acceso en línea: | http://hdl.handle.net/10486/711010 https://dx.doi.org/10.1016/j.talanta.2023.125497 |
| Access Level: | acceso abierto |
| Palabra clave: | Fluorescence Molybdenum disulphide Nanostructured bioconjugate SARS-CoV-2 Tetrahedral DNA nanostructures Química |
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A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detectionGarcía Fernández, DanielGutiérrez Gálvez, LauraVázquez Sulleiro, ManuelGarrido, MarinaLópez Diego, DavidLuna, MónicaPérez, Emilio M.García Mendiola, TaniaLorenzo Abad, EncarnaciónFluorescenceMolybdenum disulphideNanostructured bioconjugateSARS-CoV-2Tetrahedral DNA nanostructuresQuímicaIn this work we present the preparation of a 2D molybdenum disulphide nanosheets (2D-MoS2) and tetrahedral DNA nanostructures (TDNs) bioconjugate, and its application to the development of a bioassay for rapid and easy virus detection. The bioconjugate has been prepared by using TDNs carrying the capture probe labelled with 6-carboxyfluoresceine (6-FAM). As case of study to assess the utility of the assay developed, we have chosen the SARS-CoV-2 virus. Hence, as probe we have used a DNA sequence complementary to a region of the SARS-CoV-2 ORF1ab gene (TDN-ORF-FAM). This 6-FAM labelled capture probe is located on the top vertex of the tetrahedral DNA nanostructure, the three left vertices of TDNs have a thiol group. These TDNs are bounded to 2D-MoS2 surface through the three thiol groups, allowing the capture probe to be oriented to favour the biorecognition reaction with the analyte. This biorecognition resulting platform has finally been challenged to the detection of the SARS-CoV-2 ORF1ab gene sequence as the target model by measuring fluorescence before and after the hybridization event with a detection limit of 19.7fM. Furthermore, due to high sensitivity of the proposed methodology, it has been applied to directly detect the virus in nasopharyngeal samples of infected patients without the need of any amplification step. The developed bioassay has a wide range of applicability since it can be applied to the detection of any pathogen by changing the probe corresponding to the target sequence. Thus, a novel, hands-on strategy for rapid pathogen detection has proposed and has a high potential application value in the early diagnosis of infections causes by virus or bacteriaPID2020-116728RB-I00, S2018/NMT-4291 TEC2SPACEElsevierDepartamento de Química Analítica y Análisis InstrumentalFacultad de Ciencias20232023-12-15research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/711010https://dx.doi.org/10.1016/j.talanta.2023.125497reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7110102026-06-23T12:46:27Z |
| dc.title.none.fl_str_mv |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| title |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| spellingShingle |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection García Fernández, Daniel Fluorescence Molybdenum disulphide Nanostructured bioconjugate SARS-CoV-2 Tetrahedral DNA nanostructures Química |
| title_short |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| title_full |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| title_fullStr |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| title_full_unstemmed |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| title_sort |
A “signal off-on” fluorescence bioassay based on 2D-MoS2-tetrahedral DNA bioconjugate for rapid virus detection |
| dc.creator.none.fl_str_mv |
García Fernández, Daniel Gutiérrez Gálvez, Laura Vázquez Sulleiro, Manuel Garrido, Marina López Diego, David Luna, Mónica Pérez, Emilio M. García Mendiola, Tania Lorenzo Abad, Encarnación |
| author |
García Fernández, Daniel |
| author_facet |
García Fernández, Daniel Gutiérrez Gálvez, Laura Vázquez Sulleiro, Manuel Garrido, Marina López Diego, David Luna, Mónica Pérez, Emilio M. García Mendiola, Tania Lorenzo Abad, Encarnación |
| author_role |
author |
| author2 |
Gutiérrez Gálvez, Laura Vázquez Sulleiro, Manuel Garrido, Marina López Diego, David Luna, Mónica Pérez, Emilio M. García Mendiola, Tania Lorenzo Abad, Encarnación |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Química Analítica y Análisis Instrumental Facultad de Ciencias |
| dc.subject.none.fl_str_mv |
Fluorescence Molybdenum disulphide Nanostructured bioconjugate SARS-CoV-2 Tetrahedral DNA nanostructures Química |
| topic |
Fluorescence Molybdenum disulphide Nanostructured bioconjugate SARS-CoV-2 Tetrahedral DNA nanostructures Química |
| description |
In this work we present the preparation of a 2D molybdenum disulphide nanosheets (2D-MoS2) and tetrahedral DNA nanostructures (TDNs) bioconjugate, and its application to the development of a bioassay for rapid and easy virus detection. The bioconjugate has been prepared by using TDNs carrying the capture probe labelled with 6-carboxyfluoresceine (6-FAM). As case of study to assess the utility of the assay developed, we have chosen the SARS-CoV-2 virus. Hence, as probe we have used a DNA sequence complementary to a region of the SARS-CoV-2 ORF1ab gene (TDN-ORF-FAM). This 6-FAM labelled capture probe is located on the top vertex of the tetrahedral DNA nanostructure, the three left vertices of TDNs have a thiol group. These TDNs are bounded to 2D-MoS2 surface through the three thiol groups, allowing the capture probe to be oriented to favour the biorecognition reaction with the analyte. This biorecognition resulting platform has finally been challenged to the detection of the SARS-CoV-2 ORF1ab gene sequence as the target model by measuring fluorescence before and after the hybridization event with a detection limit of 19.7fM. Furthermore, due to high sensitivity of the proposed methodology, it has been applied to directly detect the virus in nasopharyngeal samples of infected patients without the need of any amplification step. The developed bioassay has a wide range of applicability since it can be applied to the detection of any pathogen by changing the probe corresponding to the target sequence. Thus, a novel, hands-on strategy for rapid pathogen detection has proposed and has a high potential application value in the early diagnosis of infections causes by virus or bacteria |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023-12-15 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10486/711010 https://dx.doi.org/10.1016/j.talanta.2023.125497 |
| url |
http://hdl.handle.net/10486/711010 https://dx.doi.org/10.1016/j.talanta.2023.125497 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
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Universidad Autónoma de Madrid |
| reponame_str |
Biblos-e Archivo. Repositorio Institucional de la UAM |
| collection |
Biblos-e Archivo. Repositorio Institucional de la UAM |
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| repository.mail.fl_str_mv |
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15,301603 |