MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: Application in viral infection diagnostics
An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modifed carbon nanodots (CNDsTy). The innovation of this work lies in the frst-time integration of these nanomaterials for...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/718710 |
| Acceso en línea: | http://hdl.handle.net/10486/718710 https://dx.doi.org/10.1007/s00604-025-07084-2 |
| Access Level: | acceso abierto |
| Palabra clave: | Bioconjugate Biosensor Diferential Pulse Voltammetry Molybdenum Disulphide Tetrahedral DNA Nanostructures Thionine-Modifed Carbon Nanodots Química |
| Sumario: | An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modifed carbon nanodots (CNDsTy). The innovation of this work lies in the frst-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor’s functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identifcation via genetic code recognition. CNDsTy allow the electrochemical detection based on their diferent afnity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identifcation. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples |
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