Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage

Financiación de acceso abierto proporcionada por los Fondos Europeos FEDER y la Junta de Castilla y León en el marco de la Estrategia de Investigación e Innovación para la Especialización Inteligente (RIS3) de Castilla y León 2021-2027

Detalhes bibliográficos
Autores: Albornos, Lucía, Iriondo, Paula, Dopico, Berta, Martín Sánchez, José Ignacio
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/166999
Acesso em linha:http://hdl.handle.net/10366/166999
Access Level:acceso abierto
Palavra-chave:β-galactosidase
Arabidopsis
Arabinogalactan proteins
Cell Wall
Seed coat mucilage
Type II arabinogalactan
2417.03 Botánica General
2417.19 Fisiología Vegetal
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oai_identifier_str oai:gredos.usal.es:10366/166999
network_acronym_str ES
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repository_id_str
spelling Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilageAlbornos, LucíaIriondo, PaulaDopico, BertaMartín Sánchez, José Ignacioβ-galactosidaseArabidopsisArabinogalactan proteinsCell WallSeed coat mucilageType II arabinogalactan2417.03 Botánica General2417.19 Fisiología VegetalFinanciación de acceso abierto proporcionada por los Fondos Europeos FEDER y la Junta de Castilla y León en el marco de la Estrategia de Investigación e Innovación para la Especialización Inteligente (RIS3) de Castilla y León 2021-2027Arabinogalactan proteins (AGPs) are involved in various physiological processes, such as cell elongation, xylem differentiation, resistance to abiotic stresses or secretion and adherence of seed coat mucilage, a structure suggested as a model system for cell wall studies. The specific roles of AGPs are not fully established, although their carbohydrate motif (type II arabinogalactan, AGII) seems to be essential, being able to mediate interactions with different signalling molecules or with other cell wall polysaccharides. The aim of the present work is to determine the role of AGII from AGPs in the structural organization of the cell wall, using Arabidopsis thaliana plants that overproduce β-galactosidase βV-Gal from Cicer arietinum (35S::βV-Gal plants), an enzyme that acts specifically on the β-(1,3) and β-(1,6)-galactosyl bonds of AGII. The characterization of the seed coat mucilage has allowed us to establish a cell wall homeostasis mechanism in which the neutral side chains of the AGII of the AGPs determine the degree of HG methyl esterification. Thus, the reduction in the galactose is accompanied by an increase in the level of esterification, probably as a compensatory mechanism to maintain the mechanical properties of this specialized cell wall and its hydration properties.Open access funding provided by FEDER European Funds and the Junta de Castilla y León under the Research and Innovation Strategy for Smart Specialization (RIS3) of Castilla y León 2021-2027. Junta de Castilla y León, SA027G18Springer202520252025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/166999reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésSA027G18Attribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1669992026-06-07T06:28:51Z
dc.title.none.fl_str_mv Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
title Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
spellingShingle Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
Albornos, Lucía
β-galactosidase
Arabidopsis
Arabinogalactan proteins
Cell Wall
Seed coat mucilage
Type II arabinogalactan
2417.03 Botánica General
2417.19 Fisiología Vegetal
title_short Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
title_full Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
title_fullStr Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
title_full_unstemmed Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
title_sort Reduction of galactose side chains in type II arabinogalactan alters homogalacturonan methyl esterification in Arabidopsis thaliana seed coat mucilage
dc.creator.none.fl_str_mv Albornos, Lucía
Iriondo, Paula
Dopico, Berta
Martín Sánchez, José Ignacio
author Albornos, Lucía
author_facet Albornos, Lucía
Iriondo, Paula
Dopico, Berta
Martín Sánchez, José Ignacio
author_role author
author2 Iriondo, Paula
Dopico, Berta
Martín Sánchez, José Ignacio
author2_role author
author
author
dc.subject.none.fl_str_mv β-galactosidase
Arabidopsis
Arabinogalactan proteins
Cell Wall
Seed coat mucilage
Type II arabinogalactan
2417.03 Botánica General
2417.19 Fisiología Vegetal
topic β-galactosidase
Arabidopsis
Arabinogalactan proteins
Cell Wall
Seed coat mucilage
Type II arabinogalactan
2417.03 Botánica General
2417.19 Fisiología Vegetal
description Financiación de acceso abierto proporcionada por los Fondos Europeos FEDER y la Junta de Castilla y León en el marco de la Estrategia de Investigación e Innovación para la Especialización Inteligente (RIS3) de Castilla y León 2021-2027
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/166999
url http://hdl.handle.net/10366/166999
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv SA027G18
dc.rights.none.fl_str_mv Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869422008275566592
score 15,812429

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