Interplay of leucocyte-platelet complexes and clinical response to eltrombopag in immune thrombocytopenia patients

Eltrombopag (ELT) is a thrombopoietin-receptor agonist that stimulates platelet (PLT) production in patients with primary immune thrombocytopenia (ITP). One potential mechanism of ELT is modulating the inflammatory response by increasing PLTs binding to leucocytes. This study examined the effect of...

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Detalles Bibliográficos
Autores: Osuna-Gómez, R, Zamora, C, Novelli, S, Garcia-Pallarols, F, Rodriguez, Y, Domingo, A, Canet, M, Olivera, P, Mulet, M, Cantó, E, Valcarcel, D, Sanchez-Gonzalez, B, Vidal, S
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p18384
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=18384
Access Level:acceso abierto
Palabra clave:autoimmune disease
cellular immunology
immunology
immunophenotyping
platelet immunology
thrombocytopenia
Descripción
Sumario:Eltrombopag (ELT) is a thrombopoietin-receptor agonist that stimulates platelet (PLT) production in patients with primary immune thrombocytopenia (ITP). One potential mechanism of ELT is modulating the inflammatory response by increasing PLTs binding to leucocytes. This study examined the effect of ELT on leucocyte-PLTs complexes in 38 ITP patients. Patients, predominantly females with a mean age of 59 years, underwent treatments like corticosteroids, intravenous immunoglobulin and splenectomy. Compared to healthy donors, ITP patients exhibited lower percentages of lymphocyte with bound PLTs, but similar monocyte- or neutrophil with bound PLTs. ELT treatment increased PLTs counts and all types of leucocyte with bound PLTs. Network analysis showed dynamic changes in leucocyte with bound PLTs relationships due to ELT. Machine learning indicated that higher percentages of monocytes with bound PLTs were linked to a better clinical response to ELT. A possible mechanism was an increased IL-10 production in monocytes with bound PLTs from responder patients. This study provides insights into the immunological changes in ITP patients undergoing ELT and suggests potential predictive biomarkers for treatment response and disease monitoring. In the peripheral blood of immune thrombocytopenia (ITP) patients who respond to eltrombopag, the percentage of monocytes with bound platelets (PLTs) is higher compared to non-responder patients. These monocytes with bound PLT showed increased IL-10 production and higher CD16 and CD14 expression compared to monocytes without bound PLTs. This indicates better platelet recovery and a more durable response in ITP patients (created with ).image