Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives

Electrokinetic chromatography with cyclodextrin derivatives (CD-EKC) was used to achieve the rapid enantiomeric separation of chiral polychlorinated biphenyls (PCBs). Thirteen of the 19 chiral PCBs stable at room temperature were individually separated into their two enantiomers by using 2-morpholin...

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Autores: García Ruiz, Carmen|||0000-0001-5925-3449, Martín Biosca, Yolanda, Crego Navazo, Antonio Luis|||0000-0002-4083-5294, Marina Alegre, María Luisa|||0000-0002-5583-1624
Tipo de documento: artigo
Data de publicação:2001
País:España
Recursos:Universidad de Alcalá (UAH)
Repositório:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglês
OAI Identifier:oai:ebuah.uah.es:10017/1354
Acesso em linha:http://hdl.handle.net/10017/1354
https://dx.doi.org/10.1016/S0021-9673(00)01176-6
Access Level:Acceso aberto
Palavra-chave:Enantiomer separation
Buffer composition
Polychlorinated biphenyls
Cyclodextrins
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
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spelling Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivativesGarcía Ruiz, Carmen|||0000-0001-5925-3449Martín Biosca, YolandaCrego Navazo, Antonio Luis|||0000-0002-4083-5294Marina Alegre, María Luisa|||0000-0002-5583-1624Enantiomer separationBuffer compositionPolychlorinated biphenylsCyclodextrinsCienciaQuímica analítica e industrialScienceChemistry, analytic and technicalElectrokinetic chromatography with cyclodextrin derivatives (CD-EKC) was used to achieve the rapid enantiomeric separation of chiral polychlorinated biphenyls (PCBs). Thirteen of the 19 chiral PCBs stable at room temperature were individually separated into their two enantiomers by using 2-morpholinoethanesulfonic acid (MES) buffer (pH 6.5) containing carboxymethylated g-cyclodextrin (CM-g-CD) as pseudostationary phase mixed with b-cyclodextrin (b-CD) or permethylated b-cyclodextrin (PM-b-CD). Urea was also added to increase the solubility of PCBs and cyclodextrins in the aqueous separation buffer. Several experimental parameters such as the nature, concentration, and pH of the buffer, nature and concentration of the cyclodextrin derivatives used, and the addition of different additives were studied in order to improve the enantiomeric separation. In addition, the effect of some instrumental parameters such as separation temperature and applied voltage was also investigated. PCBs were enantiomerically separated in less than 12 min by using a 50 mM MES buffer (pH 6.5) containing 20 mM CM-g-CD, 10 mM b-CD or 20 mM PM-b-CD, and 2 M urea at a temperature of 458C and an applied voltage of 20 kV.Elsevier20012001-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/1354https://dx.doi.org/10.1016/S0021-9673(00)01176-6reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/13542026-06-18T11:13:07Z
dc.title.none.fl_str_mv Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
title Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
spellingShingle Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
García Ruiz, Carmen|||0000-0001-5925-3449
Enantiomer separation
Buffer composition
Polychlorinated biphenyls
Cyclodextrins
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
title_short Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
title_full Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
title_fullStr Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
title_full_unstemmed Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
title_sort Rapid enantiomeric separation of polychlorinated biphenyls by electrokinetic chromatography using mixtures of neutral and charged cyclodextrin derivatives
dc.creator.none.fl_str_mv García Ruiz, Carmen|||0000-0001-5925-3449
Martín Biosca, Yolanda
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
Marina Alegre, María Luisa|||0000-0002-5583-1624
author García Ruiz, Carmen|||0000-0001-5925-3449
author_facet García Ruiz, Carmen|||0000-0001-5925-3449
Martín Biosca, Yolanda
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
Marina Alegre, María Luisa|||0000-0002-5583-1624
author_role author
author2 Martín Biosca, Yolanda
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
Marina Alegre, María Luisa|||0000-0002-5583-1624
author2_role author
author
author
dc.subject.none.fl_str_mv Enantiomer separation
Buffer composition
Polychlorinated biphenyls
Cyclodextrins
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
topic Enantiomer separation
Buffer composition
Polychlorinated biphenyls
Cyclodextrins
Ciencia
Química analítica e industrial
Science
Chemistry, analytic and technical
description Electrokinetic chromatography with cyclodextrin derivatives (CD-EKC) was used to achieve the rapid enantiomeric separation of chiral polychlorinated biphenyls (PCBs). Thirteen of the 19 chiral PCBs stable at room temperature were individually separated into their two enantiomers by using 2-morpholinoethanesulfonic acid (MES) buffer (pH 6.5) containing carboxymethylated g-cyclodextrin (CM-g-CD) as pseudostationary phase mixed with b-cyclodextrin (b-CD) or permethylated b-cyclodextrin (PM-b-CD). Urea was also added to increase the solubility of PCBs and cyclodextrins in the aqueous separation buffer. Several experimental parameters such as the nature, concentration, and pH of the buffer, nature and concentration of the cyclodextrin derivatives used, and the addition of different additives were studied in order to improve the enantiomeric separation. In addition, the effect of some instrumental parameters such as separation temperature and applied voltage was also investigated. PCBs were enantiomerically separated in less than 12 min by using a 50 mM MES buffer (pH 6.5) containing 20 mM CM-g-CD, 10 mM b-CD or 20 mM PM-b-CD, and 2 M urea at a temperature of 458C and an applied voltage of 20 kV.
publishDate 2001
dc.date.none.fl_str_mv 2001
2001-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/1354
https://dx.doi.org/10.1016/S0021-9673(00)01176-6
url http://hdl.handle.net/10017/1354
https://dx.doi.org/10.1016/S0021-9673(00)01176-6
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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