Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic

A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, Tubifex tubifex (Annelida, Clitellata). Several toxicological endpoints...

Descripción completa

Detalles Bibliográficos
Autores: Moreno Ocio, Iñigo, Aquilino, Mónica, Llorente, Lola, Martínez Madrid, Maite, Rodríguez Rodríguez, María Pilar, Méndez Fernández, Leire, Planelló, Rosario
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/66544
Acceso en línea:http://hdl.handle.net/10810/66544
Access Level:acceso abierto
Palabra clave:arsenic
oligochaeta
toxicity assay
transcriptional biomarker
cell stress response
oxidative stress
homeostasis
effect concentration
reference transcriptome
id ES_dd9feff9af72a650ef6d7513d1db9bcf
oai_identifier_str oai:addi.ehu.eus:10810/66544
network_acronym_str ES
network_name_str España
repository_id_str
spelling Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to ArsenicMoreno Ocio, IñigoAquilino, MónicaLlorente, LolaMartínez Madrid, MaiteRodríguez Rodríguez, María PilarMéndez Fernández, LeirePlanelló, Rosarioarsenicoligochaetatoxicity assaytranscriptional biomarkercell stress responseoxidative stresshomeostasiseffect concentrationreference transcriptomeA toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, Tubifex tubifex (Annelida, Clitellata). Several toxicological endpoints (survival and autotomy) of the oligochaete and tissue residues were measured, and dose-response modelling of gene expression data was studied. A reference transcriptome of the aquatic oligochaete, T. tubifex, was reconstructed for the first time, and genes related to cell stress response (Hsc70, Hsp10, Hsp60, and Hsp83), energy metabolism (COX1), oxidative stress (Cat, GSR, and MnSOD), and the genes involved in the homeostasis of organisms (CaM, RpS13, and UBE2) were identified and characterised. The potential use of the genes identified for risk assessment in freshwater ecosystems as early biomarkers of arsenic toxicity is discussed.This research was funded by CGL 2013-44655-R/BOS research project sponsored by the Spanish Ministry of Economy, Industry and Competitiveness and the UNED projects 2018I/PPRO/008. The UPV/EHU university supported the pre-doctoral internship of Íñigo Moreno at UNED. Iñigo Moreno-Ocio was financed by a Ph.D. scholarship from the University of the Basque Country (UPV/EHU) from 2017–2021 (PIF16/162). Lola Llorente was co-financed by the Community of Madrid Government and the European Social Fund with a research assistant contract (PEJ-2017-AI/BIO-6834). Mónica Aquilino was co-financed by the Community of Madrid Government and the European Social Fund with a postdoctoral contract (PEJD-2019-POST/AMB-16425). She is financed by the Spanish Ministry of Universities and the European Union-NextGenerationUE with a María Zambrano grant to attract international talent.MDPI2024202420242024info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/66544reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésihttps://www.mdpi.com/1422-0067/25/6/3382info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/es/© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).oai:addi.ehu.eus:10810/665442026-06-18T09:23:17Z
dc.title.none.fl_str_mv Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
title Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
spellingShingle Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
Moreno Ocio, Iñigo
arsenic
oligochaeta
toxicity assay
transcriptional biomarker
cell stress response
oxidative stress
homeostasis
effect concentration
reference transcriptome
title_short Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
title_full Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
title_fullStr Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
title_full_unstemmed Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
title_sort Toxicogenomics of the Freshwater Oligochaete, Tubifex tubifex (Annelida, Clitellata), in Acute Water-Only Exposure to Arsenic
dc.creator.none.fl_str_mv Moreno Ocio, Iñigo
Aquilino, Mónica
Llorente, Lola
Martínez Madrid, Maite
Rodríguez Rodríguez, María Pilar
Méndez Fernández, Leire
Planelló, Rosario
author Moreno Ocio, Iñigo
author_facet Moreno Ocio, Iñigo
Aquilino, Mónica
Llorente, Lola
Martínez Madrid, Maite
Rodríguez Rodríguez, María Pilar
Méndez Fernández, Leire
Planelló, Rosario
author_role author
author2 Aquilino, Mónica
Llorente, Lola
Martínez Madrid, Maite
Rodríguez Rodríguez, María Pilar
Méndez Fernández, Leire
Planelló, Rosario
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv arsenic
oligochaeta
toxicity assay
transcriptional biomarker
cell stress response
oxidative stress
homeostasis
effect concentration
reference transcriptome
topic arsenic
oligochaeta
toxicity assay
transcriptional biomarker
cell stress response
oxidative stress
homeostasis
effect concentration
reference transcriptome
description A toxicogenomic approach was used for toxicity evaluation of arsenic in the aquatic environment, and differential gene expression was investigated from 24 h and 96 h water-only acute toxicity tests with the aquatic oligochaete, Tubifex tubifex (Annelida, Clitellata). Several toxicological endpoints (survival and autotomy) of the oligochaete and tissue residues were measured, and dose-response modelling of gene expression data was studied. A reference transcriptome of the aquatic oligochaete, T. tubifex, was reconstructed for the first time, and genes related to cell stress response (Hsc70, Hsp10, Hsp60, and Hsp83), energy metabolism (COX1), oxidative stress (Cat, GSR, and MnSOD), and the genes involved in the homeostasis of organisms (CaM, RpS13, and UBE2) were identified and characterised. The potential use of the genes identified for risk assessment in freshwater ecosystems as early biomarkers of arsenic toxicity is discussed.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/66544
url http://hdl.handle.net/10810/66544
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv i
https://www.mdpi.com/1422-0067/25/6/3382
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421876676132864
score 15.300719

Similares en LA Referencia