Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace

Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and hi...

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Detalhes bibliográficos
Autores: Pino García, Raquel del, García Lomillo, Javier, Rivero Pérez, Maria Dolores, González San José, Mª Luisa, Muñiz Rodríguez, Pilar
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Recursos:Universidad de Burgos (UBU)
Repositorio:Repositorio Institucional de la Universidad de Burgos (RIUBU)
OAI Identifier:oai:riubu.ubu.es:10259/4294
Acesso em linha:http://hdl.handle.net/10259/4294
Access Level:acceso abierto
Palavra-chave:antioxidant activities
ABTS
Folin−Ciocalteu
free radical scavenging assays
cellulose
Food
Biochemistry
Molecular biology
Alimentos
Bioquímica
Biología molecular
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spelling Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine PomacePino García, Raquel delGarcía Lomillo, JavierRivero Pérez, Maria DoloresGonzález San José, Mª LuisaMuñiz Rodríguez, Pilarantioxidant activitiesABTSFolin−Ciocalteufree radical scavenging assayscelluloseFoodBiochemistryMolecular biologyAlimentosBioquímicaBiología molecularEvaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.The autonomous government of Castilla y León (Project BU268A11-2)American Chemical Society201620162015info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10259/4294reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)instname:Universidad de Burgos (UBU)InglésJournal of Agricultural and Food Chemistry. 2015, V. 63, n. 31, p. 6835-7094http://dx.doi.org/10.1021/jf5042678info:eu-repo/semantics/openAccessoai:riubu.ubu.es:10259/42942026-05-28T07:56:11Z
dc.title.none.fl_str_mv Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
title Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
spellingShingle Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
Pino García, Raquel del
antioxidant activities
ABTS
Folin−Ciocalteu
free radical scavenging assays
cellulose
Food
Biochemistry
Molecular biology
Alimentos
Bioquímica
Biología molecular
title_short Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
title_full Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
title_fullStr Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
title_full_unstemmed Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
title_sort Adaptation and Validation of QUick, Easy, New, CHEap, and Reproducible (QUENCHER) Antioxidant Capacity Assays in Model Products Obtained from Residual Wine Pomace
dc.creator.none.fl_str_mv Pino García, Raquel del
García Lomillo, Javier
Rivero Pérez, Maria Dolores
González San José, Mª Luisa
Muñiz Rodríguez, Pilar
author Pino García, Raquel del
author_facet Pino García, Raquel del
García Lomillo, Javier
Rivero Pérez, Maria Dolores
González San José, Mª Luisa
Muñiz Rodríguez, Pilar
author_role author
author2 García Lomillo, Javier
Rivero Pérez, Maria Dolores
González San José, Mª Luisa
Muñiz Rodríguez, Pilar
author2_role author
author
author
author
dc.subject.none.fl_str_mv antioxidant activities
ABTS
Folin−Ciocalteu
free radical scavenging assays
cellulose
Food
Biochemistry
Molecular biology
Alimentos
Bioquímica
Biología molecular
topic antioxidant activities
ABTS
Folin−Ciocalteu
free radical scavenging assays
cellulose
Food
Biochemistry
Molecular biology
Alimentos
Bioquímica
Biología molecular
description Evaluation of the total antioxidant capacity of solid matrices without extraction steps is a very interesting alternative for food researchers and also for food industries. These methodologies have been denominated QUENCHER from QUick, Easy, New, CHEap, and Reproducible assays. To demonstrate and highlight the validity of QUENCHER (Q) methods, values of Q-method validation were showed for the first time, and they were tested with products of well-known different chemical properties. Furthermore, new QUENCHER assays to measure scavenging capacity against superoxide, hydroxyl, and lipid peroxyl radicals were developed. Calibration models showed good linearity (R2 > 0.995), proportionality and precision (CV < 6.5%), and acceptable detection limits (<20.4 nmol Trolox equiv). The presence of ethanol in the reaction medium gave antioxidant capacity values significantly different from those obtained with water. The dilution of samples with powdered cellulose was discouraged because possible interferences with some of the matrices analyzed may take place.
publishDate 2015
dc.date.none.fl_str_mv 2015
2016
2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10259/4294
url http://hdl.handle.net/10259/4294
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Journal of Agricultural and Food Chemistry. 2015, V. 63, n. 31, p. 6835-7094
http://dx.doi.org/10.1021/jf5042678
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad de Burgos (RIUBU)
instname:Universidad de Burgos (UBU)
instname_str Universidad de Burgos (UBU)
reponame_str Repositorio Institucional de la Universidad de Burgos (RIUBU)
collection Repositorio Institucional de la Universidad de Burgos (RIUBU)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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