Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain
Hydroxytyrosol (HT) is the component primarily responsible for the neuroprotective effect of extra virgin olive oil (EVOO). However, it is less effective on its own than the demonstrated neuroprotective effect of EVOO, and for this reason, it can be postulated that there is an interaction between se...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Instituto de Salud Carlos III (ISCIII) |
| Repositorio: | Repisalud |
| Idioma: | inglés |
| OAI Identifier: | oai:repisalud.isciii.es:20.500.12105/18447 |
| Acceso en línea: | http://hdl.handle.net/20.500.12105/18447 |
| Access Level: | acceso abierto |
| Palabra clave: | Hydroxytyrosol Extra virgin olive oil Polyphenols In vitro Neuroprotection Aceite de oliva Polifenoles Neuroprotección Animals Rats Antioxidants 3-Methoxy-4-hydroxyphenylethanol Neuroprotective Agents Peroxynitrous Acid Brain Cell Death |
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Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat BrainDe La Cruz Cortés, José PedroPérez de Algaba, InmaculadaMartín-Aurioles, EstherArrebola, María MonsaludOrtega-Hombrados, LauraRodríguez-Pérez, María DoloresFernández-Prior, María ÁfricaBermúdez-Oria, AlejandraVerdugo, CristinaGonzález-Correa, José AntonioHydroxytyrosolExtra virgin olive oilPolyphenolsIn vitroNeuroprotectionAceite de olivaPolifenolesNeuroprotecciónAnimalsRatsAntioxidantsPolyphenols3-Methoxy-4-hydroxyphenylethanolNeuroprotective AgentsPeroxynitrous AcidBrainCell DeathHydroxytyrosol (HT) is the component primarily responsible for the neuroprotective effect of extra virgin olive oil (EVOO). However, it is less effective on its own than the demonstrated neuroprotective effect of EVOO, and for this reason, it can be postulated that there is an interaction between several of the polyphenols of EVOO. The objective of the study was to assess the possible interaction of four EVOO polyphenols (HT, tyrosol, dihydroxyphenylglycol, and oleocanthal) in an experimental model of hypoxia-reoxygenation in rat brain slices. The lactate dehydrogenase (LDH) efflux, lipid peroxidation, and peroxynitrite production were determined as measures of cell death, oxidative stress, and nitrosative stress, respectively. First, the polyphenols were incubated with the brain slices in the same proportions that exist in EVOO, comparing their effects with those of HT. In all cases, the cytoprotective and antioxidant effects of the combination were greater than those of HT alone. Second, we calculated the concentration-effect curves for HT in the absence or presence of each polyphenol. Tyrosol did not significantly modify any of the variables inhibited by HT. Dihydroxyphenylglycol only increased the cytoprotective effect of HT at 10 µM, while it increased its antioxidant effect at 50 and 100 µM and its inhibitory effect on peroxynitrite formation at all the concentrations tested. Oleocanthal increased the cytoprotective and antioxidant effects of HT but did not modify its inhibitory effect on nitrosative stress. The results of this study show that the EVOO polyphenols DHPG and OLC increase the cytoprotective effect of HT in an experimental model of hypoxia-reoxygenation in rat brain slices, mainly due to a possibly synergistic effect on HT's antioxidant action. These results could explain the greater neuroprotective effect of EVOO than of the polyphenols alone.Multidisciplinary Digital Publishing Institute (MDPI)[De La Cruz Cortés,JP; Ortega-Hombrados,L; Rodríguez-Pérez,MD; Verdugo,C; González-Correa,JA] Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga, Málaga, Spain. [Pérez de Algaba,I] Clinical Laboratory Department, Hospital Público de Montilla, Córdoba, Spain. [Martín-Aurioles,E] UGC La Roca, Distrito Sanitario Málaga-Guadalhorce, Málaga, Spain. [Arrebola,MM] UGC Laboratorio Clínico, Hospital de la Axarquía, AGSEMA, Málaga, Spain. [Fernández-Prior,MÁ; Bermúdez-Oria,A] Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain.20242024-02-1920212021-08-2620212021-08-26research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.12105/18447reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/184472026-06-12T12:43:37Z |
| dc.title.none.fl_str_mv |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| title |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| spellingShingle |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain De La Cruz Cortés, José Pedro Hydroxytyrosol Extra virgin olive oil Polyphenols In vitro Neuroprotection Aceite de oliva Polifenoles Neuroprotección Animals Rats Antioxidants Polyphenols 3-Methoxy-4-hydroxyphenylethanol Neuroprotective Agents Peroxynitrous Acid Brain Cell Death |
| title_short |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| title_full |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| title_fullStr |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| title_full_unstemmed |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| title_sort |
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain |
| dc.creator.none.fl_str_mv |
De La Cruz Cortés, José Pedro Pérez de Algaba, Inmaculada Martín-Aurioles, Esther Arrebola, María Monsalud Ortega-Hombrados, Laura Rodríguez-Pérez, María Dolores Fernández-Prior, María África Bermúdez-Oria, Alejandra Verdugo, Cristina González-Correa, José Antonio |
| author |
De La Cruz Cortés, José Pedro |
| author_facet |
De La Cruz Cortés, José Pedro Pérez de Algaba, Inmaculada Martín-Aurioles, Esther Arrebola, María Monsalud Ortega-Hombrados, Laura Rodríguez-Pérez, María Dolores Fernández-Prior, María África Bermúdez-Oria, Alejandra Verdugo, Cristina González-Correa, José Antonio |
| author_role |
author |
| author2 |
Pérez de Algaba, Inmaculada Martín-Aurioles, Esther Arrebola, María Monsalud Ortega-Hombrados, Laura Rodríguez-Pérez, María Dolores Fernández-Prior, María África Bermúdez-Oria, Alejandra Verdugo, Cristina González-Correa, José Antonio |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
[De La Cruz Cortés,JP; Ortega-Hombrados,L; Rodríguez-Pérez,MD; Verdugo,C; González-Correa,JA] Departmento de Farmacología, Facultad de Medicina, Instituto de Investigación Biomédica (IBIMA), Universidad de Málaga, Málaga, Spain. [Pérez de Algaba,I] Clinical Laboratory Department, Hospital Público de Montilla, Córdoba, Spain. [Martín-Aurioles,E] UGC La Roca, Distrito Sanitario Málaga-Guadalhorce, Málaga, Spain. [Arrebola,MM] UGC Laboratorio Clínico, Hospital de la Axarquía, AGSEMA, Málaga, Spain. [Fernández-Prior,MÁ; Bermúdez-Oria,A] Instituto de la Grasa, Consejo Superior de Investigaciones Científicas (CSIC), Seville, Spain. |
| dc.subject.none.fl_str_mv |
Hydroxytyrosol Extra virgin olive oil Polyphenols In vitro Neuroprotection Aceite de oliva Polifenoles Neuroprotección Animals Rats Antioxidants Polyphenols 3-Methoxy-4-hydroxyphenylethanol Neuroprotective Agents Peroxynitrous Acid Brain Cell Death |
| topic |
Hydroxytyrosol Extra virgin olive oil Polyphenols In vitro Neuroprotection Aceite de oliva Polifenoles Neuroprotección Animals Rats Antioxidants Polyphenols 3-Methoxy-4-hydroxyphenylethanol Neuroprotective Agents Peroxynitrous Acid Brain Cell Death |
| description |
Hydroxytyrosol (HT) is the component primarily responsible for the neuroprotective effect of extra virgin olive oil (EVOO). However, it is less effective on its own than the demonstrated neuroprotective effect of EVOO, and for this reason, it can be postulated that there is an interaction between several of the polyphenols of EVOO. The objective of the study was to assess the possible interaction of four EVOO polyphenols (HT, tyrosol, dihydroxyphenylglycol, and oleocanthal) in an experimental model of hypoxia-reoxygenation in rat brain slices. The lactate dehydrogenase (LDH) efflux, lipid peroxidation, and peroxynitrite production were determined as measures of cell death, oxidative stress, and nitrosative stress, respectively. First, the polyphenols were incubated with the brain slices in the same proportions that exist in EVOO, comparing their effects with those of HT. In all cases, the cytoprotective and antioxidant effects of the combination were greater than those of HT alone. Second, we calculated the concentration-effect curves for HT in the absence or presence of each polyphenol. Tyrosol did not significantly modify any of the variables inhibited by HT. Dihydroxyphenylglycol only increased the cytoprotective effect of HT at 10 µM, while it increased its antioxidant effect at 50 and 100 µM and its inhibitory effect on peroxynitrite formation at all the concentrations tested. Oleocanthal increased the cytoprotective and antioxidant effects of HT but did not modify its inhibitory effect on nitrosative stress. The results of this study show that the EVOO polyphenols DHPG and OLC increase the cytoprotective effect of HT in an experimental model of hypoxia-reoxygenation in rat brain slices, mainly due to a possibly synergistic effect on HT's antioxidant action. These results could explain the greater neuroprotective effect of EVOO than of the polyphenols alone. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021-08-26 2021 2021-08-26 2024 2024-02-19 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12105/18447 |
| url |
http://hdl.handle.net/20.500.12105/18447 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute (MDPI) |
| dc.source.none.fl_str_mv |
reponame:Repisalud instname:Instituto de Salud Carlos III (ISCIII) |
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Instituto de Salud Carlos III (ISCIII) |
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Repisalud |
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Repisalud |
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|
| repository.mail.fl_str_mv |
|
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1869421806463483904 |
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15.81155 |