Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects

We studied whether myo-inositol supplementation throughout lactation, alone and combined with leptin, may reverse detrimental effects on hypothalamic structure and function caused by gestational calorie gestation (CR) in rats. Candidate early transcript-based biomarkers of metabolic health in periph...

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Detalles Bibliográficos
Autores: Castillo, Pedro, Palou, Mariona, Yau-Qiu, Zhi Xin, Rodriguez, Ana María, Palou, Andreu, Picó, Catalina
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Conselleria de Salut i Consum del Govern de les Illes Balears
Repositorio:Docusalut
Idioma:inglés
OAI Identifier:oai:docusalut.com:20.500.13003/19701
Acceso en línea:https://hdl.handle.net/20.500.13003/19701
Access Level:acceso abierto
Palabra clave:Animals, Suckling
Leukocytes, Mononuclear
Lactation
Pregnancy
Male
Inositol
Rats, Wistar
Hypothalamus
Leptin
Female
Rats
Animals
Caloric Restriction
Receptors, Leptin
Dietary Supplements
Prenatal Exposure Delayed Effects
Animales
Ratas Wistar
Femenino
Hipotálamo
Masculino
Restricción Calórica
Receptores de Leptina
Lactancia
Ratas
Leucocitos Mononucleares
Embarazo
Animales Lactantes
Suplementos Dietéticos
Leptina
Efectos Tardíos de la Exposición Prenatal
fetal undernutrition
myo-inositol
leptin
lactation period
metabolic programming
arcuate nucleus
paraventricular nucleus
PBMC
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spelling Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin EffectsCastillo, PedroPalou, MarionaYau-Qiu, Zhi XinRodriguez, Ana MaríaPalou, AndreuPicó, CatalinaAnimals, SucklingLeukocytes, MononuclearLactationPregnancyMaleInositolRats, WistarHypothalamusLeptinFemaleRatsAnimalsCaloric RestrictionReceptors, LeptinDietary SupplementsPrenatal Exposure Delayed EffectsAnimalesRatas WistarFemeninoInositolHipotálamoMasculinoRestricción CalóricaReceptores de LeptinaLactanciaRatasLeucocitos MononuclearesEmbarazoAnimales LactantesSuplementos DietéticosLeptinaEfectos Tardíos de la Exposición Prenatalfetal undernutritionmyo-inositolleptinlactation periodmetabolic programmingarcuate nucleusparaventricular nucleusPBMCWe studied whether myo-inositol supplementation throughout lactation, alone and combined with leptin, may reverse detrimental effects on hypothalamic structure and function caused by gestational calorie gestation (CR) in rats. Candidate early transcript-based biomarkers of metabolic health in peripheral blood mononuclear cells (PBMC) were also studied. offspring of dams exposed to 25% gestational CR and supplemented during lactation with physiological doses of leptin (CR-L), myo-inositol (CR-M), the combination (CR-LM), or the vehicle (CR-V) as well as control rats (CON-V) were followed and sacrificed at postnatal day 25. Myo-inositol and the combination increased the number of neurons in arcuate nucleus (ARC) (only in females) and paraventricular nucleus, and myo-inositol (alone) restored the number of alpha MSH+ neurons in ARC. Hypothalamic mRNA levels of Lepr in CR-M and Insr in CR-M and CR-LM males were higher than in CR-V and CON-V, respectively. In PBMC, increased expression levels of Lrp11 and Gls in CR-V were partially normalized in all supplemented groups (but only in males for Gls). Therefore, myo-inositol supplementation throughout lactation, alone and combined with leptin, reverts programmed alterations by fetal undernutrition on hypothalamic structure and gene expression of potential early biomarkers of metabolic health in PBMC, which might be attributed, in part, to increased leptin sensitivity.MDPI20212021-09-0120212021-09-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.13003/19701reponame:Docusalutinstname:Conselleria de Salut i Consum del Govern de les Illes BalearsInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docusalut.com:20.500.13003/197012026-06-22T12:44:07Z
dc.title.none.fl_str_mv Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
title Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
spellingShingle Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
Castillo, Pedro
Animals, Suckling
Leukocytes, Mononuclear
Lactation
Pregnancy
Male
Inositol
Rats, Wistar
Hypothalamus
Leptin
Female
Rats
Animals
Caloric Restriction
Receptors, Leptin
Dietary Supplements
Prenatal Exposure Delayed Effects
Animales
Ratas Wistar
Femenino
Inositol
Hipotálamo
Masculino
Restricción Calórica
Receptores de Leptina
Lactancia
Ratas
Leucocitos Mononucleares
Embarazo
Animales Lactantes
Suplementos Dietéticos
Leptina
Efectos Tardíos de la Exposición Prenatal
fetal undernutrition
myo-inositol
leptin
lactation period
metabolic programming
arcuate nucleus
paraventricular nucleus
PBMC
title_short Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
title_full Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
title_fullStr Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
title_full_unstemmed Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
title_sort Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects
dc.creator.none.fl_str_mv Castillo, Pedro
Palou, Mariona
Yau-Qiu, Zhi Xin
Rodriguez, Ana María
Palou, Andreu
Picó, Catalina
author Castillo, Pedro
author_facet Castillo, Pedro
Palou, Mariona
Yau-Qiu, Zhi Xin
Rodriguez, Ana María
Palou, Andreu
Picó, Catalina
author_role author
author2 Palou, Mariona
Yau-Qiu, Zhi Xin
Rodriguez, Ana María
Palou, Andreu
Picó, Catalina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Animals, Suckling
Leukocytes, Mononuclear
Lactation
Pregnancy
Male
Inositol
Rats, Wistar
Hypothalamus
Leptin
Female
Rats
Animals
Caloric Restriction
Receptors, Leptin
Dietary Supplements
Prenatal Exposure Delayed Effects
Animales
Ratas Wistar
Femenino
Inositol
Hipotálamo
Masculino
Restricción Calórica
Receptores de Leptina
Lactancia
Ratas
Leucocitos Mononucleares
Embarazo
Animales Lactantes
Suplementos Dietéticos
Leptina
Efectos Tardíos de la Exposición Prenatal
fetal undernutrition
myo-inositol
leptin
lactation period
metabolic programming
arcuate nucleus
paraventricular nucleus
PBMC
topic Animals, Suckling
Leukocytes, Mononuclear
Lactation
Pregnancy
Male
Inositol
Rats, Wistar
Hypothalamus
Leptin
Female
Rats
Animals
Caloric Restriction
Receptors, Leptin
Dietary Supplements
Prenatal Exposure Delayed Effects
Animales
Ratas Wistar
Femenino
Inositol
Hipotálamo
Masculino
Restricción Calórica
Receptores de Leptina
Lactancia
Ratas
Leucocitos Mononucleares
Embarazo
Animales Lactantes
Suplementos Dietéticos
Leptina
Efectos Tardíos de la Exposición Prenatal
fetal undernutrition
myo-inositol
leptin
lactation period
metabolic programming
arcuate nucleus
paraventricular nucleus
PBMC
description We studied whether myo-inositol supplementation throughout lactation, alone and combined with leptin, may reverse detrimental effects on hypothalamic structure and function caused by gestational calorie gestation (CR) in rats. Candidate early transcript-based biomarkers of metabolic health in peripheral blood mononuclear cells (PBMC) were also studied. offspring of dams exposed to 25% gestational CR and supplemented during lactation with physiological doses of leptin (CR-L), myo-inositol (CR-M), the combination (CR-LM), or the vehicle (CR-V) as well as control rats (CON-V) were followed and sacrificed at postnatal day 25. Myo-inositol and the combination increased the number of neurons in arcuate nucleus (ARC) (only in females) and paraventricular nucleus, and myo-inositol (alone) restored the number of alpha MSH+ neurons in ARC. Hypothalamic mRNA levels of Lepr in CR-M and Insr in CR-M and CR-LM males were higher than in CR-V and CON-V, respectively. In PBMC, increased expression levels of Lrp11 and Gls in CR-V were partially normalized in all supplemented groups (but only in males for Gls). Therefore, myo-inositol supplementation throughout lactation, alone and combined with leptin, reverts programmed alterations by fetal undernutrition on hypothalamic structure and gene expression of potential early biomarkers of metabolic health in PBMC, which might be attributed, in part, to increased leptin sensitivity.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-09-01
2021
2021-09-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.13003/19701
url https://hdl.handle.net/20.500.13003/19701
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docusalut
instname:Conselleria de Salut i Consum del Govern de les Illes Balears
instname_str Conselleria de Salut i Consum del Govern de les Illes Balears
reponame_str Docusalut
collection Docusalut
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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