Myo-Inositol Supplementation in Suckling Rats Protects against Adverse Programming Outcomes on Hypothalamic Structure Caused by Mild Gestational Calorie Restriction, Partially Comparable to Leptin Effects

We studied whether myo-inositol supplementation throughout lactation, alone and combined with leptin, may reverse detrimental effects on hypothalamic structure and function caused by gestational calorie gestation (CR) in rats. Candidate early transcript-based biomarkers of metabolic health in periph...

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Bibliographic Details
Authors: Castillo, Pedro, Palou, Mariona, Yau-Qiu, Zhi Xin, Rodriguez, Ana María, Palou, Andreu, Picó, Catalina
Format: article
Publication Date:2021
Country:España
Institution:Conselleria de Salut i Consum del Govern de les Illes Balears
Repository:Docusalut
Language:English
OAI Identifier:oai:docusalut.com:20.500.13003/19701
Online Access:https://hdl.handle.net/20.500.13003/19701
Access Level:Open access
Keyword:Animals, Suckling
Leukocytes, Mononuclear
Lactation
Pregnancy
Male
Inositol
Rats, Wistar
Hypothalamus
Leptin
Female
Rats
Animals
Caloric Restriction
Receptors, Leptin
Dietary Supplements
Prenatal Exposure Delayed Effects
Animales
Ratas Wistar
Femenino
Hipotálamo
Masculino
Restricción Calórica
Receptores de Leptina
Lactancia
Ratas
Leucocitos Mononucleares
Embarazo
Animales Lactantes
Suplementos Dietéticos
Leptina
Efectos Tardíos de la Exposición Prenatal
fetal undernutrition
myo-inositol
leptin
lactation period
metabolic programming
arcuate nucleus
paraventricular nucleus
PBMC
Description
Summary:We studied whether myo-inositol supplementation throughout lactation, alone and combined with leptin, may reverse detrimental effects on hypothalamic structure and function caused by gestational calorie gestation (CR) in rats. Candidate early transcript-based biomarkers of metabolic health in peripheral blood mononuclear cells (PBMC) were also studied. offspring of dams exposed to 25% gestational CR and supplemented during lactation with physiological doses of leptin (CR-L), myo-inositol (CR-M), the combination (CR-LM), or the vehicle (CR-V) as well as control rats (CON-V) were followed and sacrificed at postnatal day 25. Myo-inositol and the combination increased the number of neurons in arcuate nucleus (ARC) (only in females) and paraventricular nucleus, and myo-inositol (alone) restored the number of alpha MSH+ neurons in ARC. Hypothalamic mRNA levels of Lepr in CR-M and Insr in CR-M and CR-LM males were higher than in CR-V and CON-V, respectively. In PBMC, increased expression levels of Lrp11 and Gls in CR-V were partially normalized in all supplemented groups (but only in males for Gls). Therefore, myo-inositol supplementation throughout lactation, alone and combined with leptin, reverts programmed alterations by fetal undernutrition on hypothalamic structure and gene expression of potential early biomarkers of metabolic health in PBMC, which might be attributed, in part, to increased leptin sensitivity.

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