Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina

Transmissible viral proventriculitis (TVP) and chicken proventricular necrosis virus (CPNV) affect the broiler industry globally and are emerging diseases of economic importance. Here, we present the findings of TVP from processed broiler carcasses in Argentina following marked condemnation at the p...

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Autores: Gornatti-Churria, Carlos Daniel, Majo, Natalia, Macías-Rioseco, Melissa, Valle, Rosa M., García, Patricio A., Jerry, Carmen F.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/4598
Acceso en línea:http://hdl.handle.net/20.500.12327/4598
https://doi.org/10.3390/v17040519
Access Level:acceso abierto
Palabra clave:619
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spelling Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in ArgentinaGornatti-Churria, Carlos DanielMajo, NataliaMacías-Rioseco, MelissaValle, Rosa M.García, Patricio A.Jerry, Carmen F.619Transmissible viral proventriculitis (TVP) and chicken proventricular necrosis virus (CPNV) affect the broiler industry globally and are emerging diseases of economic importance. Here, we present the findings of TVP from processed broiler carcasses in Argentina following marked condemnation at the processing plant. We studied a total of 122 abnormally presenting proventriculi at processing from 42-to-50-day-old, male Cobb500™ broiler chicken carcasses from 11 farms belonging to the same company in 13 episodes of proventriculi–gizzards condemnation between December 2021 and April 2022. The proventriculi were enlarged and pale with a widened gastric isthmus. A histopathologic lesion score system was developed based on the presence of a combination of key microscopic findings, the distribution, and the severity of the lesions. Scoring of the affected proventriculi revealed 65% (79/122) with a score of 4, 23% (28/122) with a score of 3, and 12% (15/122) with a score of 2. Focal to multifocal immunoreactivity against the VP2-CPNV antigen within the necrotic glandular epithelial cells was noted in the affected proventriculi using immunohistochemistry. We found 84.4% (103/122) of the studied proventriculi with TVP lesions grossly and microscopically scored were positive for CPNV by RT-PCR. The sequencing results of the PCR product showed a high nucleotide sequence similarity (88.97%) to previously published VP1-CPNV sequences. We confirmed CPNV infection in most of the TVP affected proventriculi in all condemnation episodes at a broiler chicken processing plant in Argentina during the studied period. This study documents TVP associated with CPNV detection at processing plants in Argentina for the first time.info:eu-repo/semantics/publishedVersionMDPIProducció AnimalSanitat Animal202520252025info:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/20.500.12327/4598https://doi.org/10.3390/v17040519reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésVirusesAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/45982026-06-16T08:51:17Z
dc.title.none.fl_str_mv Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
title Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
spellingShingle Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
Gornatti-Churria, Carlos Daniel
619
title_short Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
title_full Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
title_fullStr Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
title_full_unstemmed Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
title_sort Diagnostic Findings of Transmissible Viral Proventriculitis Associated with Chicken Proventricular Necrosis Virus in Processed Broiler Chickens in Argentina
dc.creator.none.fl_str_mv Gornatti-Churria, Carlos Daniel
Majo, Natalia
Macías-Rioseco, Melissa
Valle, Rosa M.
García, Patricio A.
Jerry, Carmen F.
author Gornatti-Churria, Carlos Daniel
author_facet Gornatti-Churria, Carlos Daniel
Majo, Natalia
Macías-Rioseco, Melissa
Valle, Rosa M.
García, Patricio A.
Jerry, Carmen F.
author_role author
author2 Majo, Natalia
Macías-Rioseco, Melissa
Valle, Rosa M.
García, Patricio A.
Jerry, Carmen F.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Transmissible viral proventriculitis (TVP) and chicken proventricular necrosis virus (CPNV) affect the broiler industry globally and are emerging diseases of economic importance. Here, we present the findings of TVP from processed broiler carcasses in Argentina following marked condemnation at the processing plant. We studied a total of 122 abnormally presenting proventriculi at processing from 42-to-50-day-old, male Cobb500™ broiler chicken carcasses from 11 farms belonging to the same company in 13 episodes of proventriculi–gizzards condemnation between December 2021 and April 2022. The proventriculi were enlarged and pale with a widened gastric isthmus. A histopathologic lesion score system was developed based on the presence of a combination of key microscopic findings, the distribution, and the severity of the lesions. Scoring of the affected proventriculi revealed 65% (79/122) with a score of 4, 23% (28/122) with a score of 3, and 12% (15/122) with a score of 2. Focal to multifocal immunoreactivity against the VP2-CPNV antigen within the necrotic glandular epithelial cells was noted in the affected proventriculi using immunohistochemistry. We found 84.4% (103/122) of the studied proventriculi with TVP lesions grossly and microscopically scored were positive for CPNV by RT-PCR. The sequencing results of the PCR product showed a high nucleotide sequence similarity (88.97%) to previously published VP1-CPNV sequences. We confirmed CPNV infection in most of the TVP affected proventriculi in all condemnation episodes at a broiler chicken processing plant in Argentina during the studied period. This study documents TVP associated with CPNV detection at processing plants in Argentina for the first time.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4598
https://doi.org/10.3390/v17040519
url http://hdl.handle.net/20.500.12327/4598
https://doi.org/10.3390/v17040519
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Viruses
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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