Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes

This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented me...

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Autores: Arcarons Deseures, Núria, Morató, Roser|||0000-0003-2020-7589, Vendrell-Flotats, Meritxell|||0000-0002-6360-9337, Yeste Oliveras, Marc|||0000-0002-2209-340X, López Béjar, Manel|||0000-0001-9490-6126, Rajapaksha, Kosala, Anzar, Muhammad
Formato: artículo
Fecha de publicación:2017
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:220822
Acesso em linha:https://ddd.uab.cat/record/220822
https://dx.doi.org/urn:doi:10.1371/journal.pone.0184714
Access Level:acceso abierto
Palavra-chave:Colesterol
Fecundació in vitro
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spelling Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytesArcarons Deseures, NúriaMorató, Roser|||0000-0003-2020-7589Vendrell-Flotats, Meritxell|||0000-0002-6360-9337Yeste Oliveras, Marc|||0000-0002-2209-340XLópez Béjar, Manel|||0000-0001-9490-6126Rajapaksha, KosalaAnzar, MuhammadColesterolFecundació in vitroThis study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of cholesterol by confocal microscopy live-cell imaging. Subsequently, the effects of optimized CLC concentrations and incubation times prior to vitrification on early embryo development were assessed. Then, we evaluated the effects of pretreatment with 2 mg/ml CLC for 30 min before the vitrification of immature (GV) and in vitro matured (MII) oocytes on developmental competence and gene expression. Our results indicate a high plasma membrane labeling intensity after 30 min of incubation with 2 mg/ml CLC for 30 min, regardless of the holding medium used. When oocytes were incubated with 1 mg/ml, 2 mg/ml and 3 mg/ml of CLC, intense labeling was observed at the plasma membrane after 40, 30 and 20 min, respectively. CLC pre-treatment before the vitrification of bovine oocytes did not affect subsequent cleavage and embryo development rates irrespective of CLC concentrations, incubation times or meiotic stage. However, pretreatment seems to improve the quality of embryos derived from vitrified oocytes, mainly when oocytes were vitrified at the GV stage.Mogas Amorós, Teresa 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/220822https://dx.doi.org/urn:doi:10.1371/journal.pone.0184714reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-46769-Popen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2208222026-06-06T12:50:31Z
dc.title.none.fl_str_mv Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
title Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
spellingShingle Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
Arcarons Deseures, Núria
Colesterol
Fecundació in vitro
title_short Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
title_full Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
title_fullStr Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
title_full_unstemmed Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
title_sort Cholesterol added prior to vitrification on the cryotolerance of immature and in vitro matured bovine oocytes
dc.creator.none.fl_str_mv Arcarons Deseures, Núria
Morató, Roser|||0000-0003-2020-7589
Vendrell-Flotats, Meritxell|||0000-0002-6360-9337
Yeste Oliveras, Marc|||0000-0002-2209-340X
López Béjar, Manel|||0000-0001-9490-6126
Rajapaksha, Kosala
Anzar, Muhammad
author Arcarons Deseures, Núria
author_facet Arcarons Deseures, Núria
Morató, Roser|||0000-0003-2020-7589
Vendrell-Flotats, Meritxell|||0000-0002-6360-9337
Yeste Oliveras, Marc|||0000-0002-2209-340X
López Béjar, Manel|||0000-0001-9490-6126
Rajapaksha, Kosala
Anzar, Muhammad
author_role author
author2 Morató, Roser|||0000-0003-2020-7589
Vendrell-Flotats, Meritxell|||0000-0002-6360-9337
Yeste Oliveras, Marc|||0000-0002-2209-340X
López Béjar, Manel|||0000-0001-9490-6126
Rajapaksha, Kosala
Anzar, Muhammad
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Mogas Amorós, Teresa
dc.subject.none.fl_str_mv Colesterol
Fecundació in vitro
topic Colesterol
Fecundació in vitro
description This study examines whether incorporating cholesterol-loaded methyl-β-cyclodextrin (CLC) in the bovine oocyte plasma membrane improves oocyte tolerance to vitrification. In vitro matured oocytes were incubated with 2 mg/ml BODIPY-labeled CLC for different time intervals in FCS or PVA supplemented medium or exposed to different CLC concentrations to examine the subcellular localization of cholesterol by confocal microscopy live-cell imaging. Subsequently, the effects of optimized CLC concentrations and incubation times prior to vitrification on early embryo development were assessed. Then, we evaluated the effects of pretreatment with 2 mg/ml CLC for 30 min before the vitrification of immature (GV) and in vitro matured (MII) oocytes on developmental competence and gene expression. Our results indicate a high plasma membrane labeling intensity after 30 min of incubation with 2 mg/ml CLC for 30 min, regardless of the holding medium used. When oocytes were incubated with 1 mg/ml, 2 mg/ml and 3 mg/ml of CLC, intense labeling was observed at the plasma membrane after 40, 30 and 20 min, respectively. CLC pre-treatment before the vitrification of bovine oocytes did not affect subsequent cleavage and embryo development rates irrespective of CLC concentrations, incubation times or meiotic stage. However, pretreatment seems to improve the quality of embryos derived from vitrified oocytes, mainly when oocytes were vitrified at the GV stage.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/220822
https://dx.doi.org/urn:doi:10.1371/journal.pone.0184714
url https://ddd.uab.cat/record/220822
https://dx.doi.org/urn:doi:10.1371/journal.pone.0184714
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2013-46769-P
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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