Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos

This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplem...

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Autores: Ordóñez-León, Erika Alina, Martínez-Rodero, Iris|||0000-0001-7057-2045, Garcia Martinez, Tania|||0000-0002-3428-3075, López Béjar, Manel|||0000-0001-9490-6126, Yeste Oliveras, Marc|||0000-0002-2209-340X, Mercadé, Elena|||0000-0001-7828-2210, Mogas Amorós, Teresa|||0000-0002-6733-1328
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:260299
Acesso em linha:https://ddd.uab.cat/record/260299
https://dx.doi.org/urn:doi:10.3390/ijms23137069
Access Level:acceso abierto
Palavra-chave:Cryopreservation
Blastocyst
Total cell number
Inner cell mass
TUNEL
Embryo development
Gene expression regulation
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spelling Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine EmbryosOrdóñez-León, Erika AlinaMartínez-Rodero, Iris|||0000-0001-7057-2045Garcia Martinez, Tania|||0000-0002-3428-3075López Béjar, Manel|||0000-0001-9490-6126Yeste Oliveras, Marc|||0000-0002-2209-340XMercadé, Elena|||0000-0001-7828-2210Mogas Amorós, Teresa|||0000-0002-6733-1328CryopreservationBlastocystTotal cell numberInner cell massTUNELEmbryo developmentGene expression regulationThis study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/260299https://dx.doi.org/urn:doi:10.3390/ijms23137069reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116531RB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BES-2017-081962open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2602992026-06-06T12:50:31Z
dc.title.none.fl_str_mv Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
title Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
spellingShingle Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
Ordóñez-León, Erika Alina
Cryopreservation
Blastocyst
Total cell number
Inner cell mass
TUNEL
Embryo development
Gene expression regulation
title_short Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
title_full Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
title_fullStr Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
title_full_unstemmed Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
title_sort Exopolysaccharide ID1 Improves Post-Warming Outcomes after Vitrification of In Vitro-Produced Bovine Embryos
dc.creator.none.fl_str_mv Ordóñez-León, Erika Alina
Martínez-Rodero, Iris|||0000-0001-7057-2045
Garcia Martinez, Tania|||0000-0002-3428-3075
López Béjar, Manel|||0000-0001-9490-6126
Yeste Oliveras, Marc|||0000-0002-2209-340X
Mercadé, Elena|||0000-0001-7828-2210
Mogas Amorós, Teresa|||0000-0002-6733-1328
author Ordóñez-León, Erika Alina
author_facet Ordóñez-León, Erika Alina
Martínez-Rodero, Iris|||0000-0001-7057-2045
Garcia Martinez, Tania|||0000-0002-3428-3075
López Béjar, Manel|||0000-0001-9490-6126
Yeste Oliveras, Marc|||0000-0002-2209-340X
Mercadé, Elena|||0000-0001-7828-2210
Mogas Amorós, Teresa|||0000-0002-6733-1328
author_role author
author2 Martínez-Rodero, Iris|||0000-0001-7057-2045
Garcia Martinez, Tania|||0000-0002-3428-3075
López Béjar, Manel|||0000-0001-9490-6126
Yeste Oliveras, Marc|||0000-0002-2209-340X
Mercadé, Elena|||0000-0001-7828-2210
Mogas Amorós, Teresa|||0000-0002-6733-1328
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cryopreservation
Blastocyst
Total cell number
Inner cell mass
TUNEL
Embryo development
Gene expression regulation
topic Cryopreservation
Blastocyst
Total cell number
Inner cell mass
TUNEL
Embryo development
Gene expression regulation
description This study aimed to assess the cryoprotectant role of exopolysaccharide (EPS) ID1, produced by Antarctic Pseudomonas sp., in the vitrification of in vitro-produced (IVP) bovine embryos. IVP day 7 (D7) and day 8 (D8) expanded blastocysts derived from cow or calf oocytes were vitrified without supplementation (EPS0) or supplemented with 10 µg/mL (EPS10) or 100 µg/mL (EPS100) EPS ID1. The effect of EPS ID1 was assessed in post-warming re-expansion and hatching rates, differential cell count, apoptosis rate, and gene expression. EPS100 re-expansion rates were significantly higher than those observed for the EPS0 and EPS10 treatments, regardless of culture length or oocyte source. EPS100 hatching rate was similar to the one of the fresh blastocysts except for those D7 blastocysts derived from calf oocytes. No differences were observed among EPS ID1 treatments when the inner cell mass, trophectoderm, and total cell number were assessed. Although apoptosis rates were higher (p ≤ 0.05) in vitrified groups compared to fresh embryos, EPS100 blastocysts had a lower number (p ≤ 0.05) of apoptotic nuclei than the EPS0 or EPS10 groups. No differences in the expression of BCL2, AQP3, CX43, and SOD1 genes between treatments were observed. Vitrification without EPS ID1 supplementation produced blastocysts with significantly higher BAX gene expression, whereas treatment with 100 µg/mL EPS ID1 returned BAX levels to those observed in non-vitrified blastocysts. Our results suggest that 100 µg/mL EPS ID1 added to the vitrification media is beneficial for embryo cryopreservation because it results in higher re-expansion and hatching ability and it positively modulates apoptosis.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/260299
https://dx.doi.org/urn:doi:10.3390/ijms23137069
url https://ddd.uab.cat/record/260299
https://dx.doi.org/urn:doi:10.3390/ijms23137069
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116531RB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BES-2017-081962
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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