Medium- and short-sized antioxidant peptides from simulated gastrointestinal digestion of muscle and hydrolysed by-products of blue whiting [DATASET]

Fish consumption is rapidly increasing worldwide, leading to increased production of related by-products and waste that can represent a suitable resource for recycling and sustainability solutions aimed at a sustainable economy. Fish-derived peptides can be exploited as food, cosmetic or pharmaceuti...

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Detalles Bibliográficos
Autores: Coppolino, Carmelo, Gallego, Marta, Mora, Leticia, Mondello, Alicia, Donato, Paola, Cerrato, Andrea, Capriotti, Anna Laura, Toldrá Vilardell, Fidel, Mondello, Luigi
Tipo de recurso: conjunto de datos
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/395038
Acceso en línea:http://hdl.handle.net/10261/395038
https://doi.org/10.20350/digitalCSIC/17418
Access Level:acceso abierto
Palabra clave:Fish by-products
Alcalase hydrolysis
Antioxidant activity
Bioactive peptides
Circular economy
Simulated gastrointestinal digestion
Mass spectrometry
antioxidant properties
bioactive compounds
fishery by-products
Descripción
Sumario:Fish consumption is rapidly increasing worldwide, leading to increased production of related by-products and waste that can represent a suitable resource for recycling and sustainability solutions aimed at a sustainable economy. Fish-derived peptides can be exploited as food, cosmetic or pharmaceutical ingredients, due to their wide variety of biological activities. In this study, the effect of simulated gastrointestinal digestion (GID) on the generation of antioxidant peptides from blue whiting muscle and from by-products hydrolysates obtained by alcalase hydrolysis and ultrasound-assisted alcalase hydrolysis was investigated. Results showed that GID led, in general, to an increase of the antioxidant activity when measured by ABTS and ORAC assays, whereas it mostly decreased when tested with DPPH and FRAP assays. A total of 1246 medium-sized and 415 short-sized peptides were identified by nano- or ultra-high-performance liquid chromatography coupled to mass spectrometry. In this context, GID led to a significant reduction in the number of identified medium-sized peptides in all samples, while increasing the quantity of short peptides identified in the digested muscle and keeping those in the by-product hydrolysates practically unchanged. Peptides containing proline, aspartate, histidine, tryptophan or glycine residues were the most resistant to GID. In silico studies of the identified peptides predicted 36 short-sized sequences resistant to GID with high potential to be bioactives. The results of this research underline the potential antioxidant activity of fish peptides obtained from GID of the edible part of the muscle. On the other hand, the retrieval and reuse of by-products with antioxidant properties provides sustainability and economical throughput, thus promoting circular economy in aquaculture.