Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE

Sulfonamides are antimicrobial agents widely employed in animal production and their residues in food could be an important risk to human health. In the dairy industry, large quantities of milk are monitored daily for the presence of sulfonamides. A simple and low-cost extraction protocol followed b...

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Detalles Bibliográficos
Autores: Nebot García, Carolina, Regal López, Patricia, Miranda López, José Manuel, Fente Sampayo, Cristina Asunción, Cepeda Sáez, Alberto
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/45563
Acceso en línea:https://hdl.handle.net/10347/45563
Access Level:acceso abierto
Palabra clave:Sulfonamides
Milk
High-performance liquid chromatography
Mass spectrometry
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spelling Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPENebot García, CarolinaRegal López, PatriciaMiranda López, José ManuelFente Sampayo, Cristina AsunciónCepeda Sáez, AlbertoSulfonamidesMilkHigh-performance liquid chromatographyMass spectrometrySulfonamides are antimicrobial agents widely employed in animal production and their residues in food could be an important risk to human health. In the dairy industry, large quantities of milk are monitored daily for the presence of sulfonamides. A simple and low-cost extraction protocol followed by a liquid chromatographic–tandem mass spectrometry method was developed for the simultaneous detection of nine sulfonamides in whole milk. The method was validated at the maximum residue limits established by European legislation. The limits of quantification obtained for most sulfonamides were between 12.5 and 25 μg kg−1, detection capabilities ranged from 116 to 145 μg kg−1, and recoveries, at 100 μg kg−1, were greater than 89 ± 12.5%. The method was employed to analyse 100 raw whole bovine milk samples collected from dairy farms in the northwest region of Spain. All of the samples were found to be compliant, but two were positive; one for sulfadiazine and the other for sulfamethoxipyridazine.ElsevierUniversidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía20132013-12-0120132013-12-01journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10347/45563reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostelainstname:Universidad de Santiago de Compostela (USC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:minerva.usc.gal:10347/455632026-06-15T12:47:27Z
dc.title.none.fl_str_mv Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
title Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
spellingShingle Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
Nebot García, Carolina
Sulfonamides
Milk
High-performance liquid chromatography
Mass spectrometry
title_short Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
title_full Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
title_fullStr Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
title_full_unstemmed Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
title_sort Rapid method for quantification of nine sulfonamides in bovine milk using HPLC/MS/MS and without using SPE
dc.creator.none.fl_str_mv Nebot García, Carolina
Regal López, Patricia
Miranda López, José Manuel
Fente Sampayo, Cristina Asunción
Cepeda Sáez, Alberto
author Nebot García, Carolina
author_facet Nebot García, Carolina
Regal López, Patricia
Miranda López, José Manuel
Fente Sampayo, Cristina Asunción
Cepeda Sáez, Alberto
author_role author
author2 Regal López, Patricia
Miranda López, José Manuel
Fente Sampayo, Cristina Asunción
Cepeda Sáez, Alberto
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de Santiago de Compostela. Departamento de Química Analítica, Nutrición e Bromatoloxía
dc.subject.none.fl_str_mv Sulfonamides
Milk
High-performance liquid chromatography
Mass spectrometry
topic Sulfonamides
Milk
High-performance liquid chromatography
Mass spectrometry
description Sulfonamides are antimicrobial agents widely employed in animal production and their residues in food could be an important risk to human health. In the dairy industry, large quantities of milk are monitored daily for the presence of sulfonamides. A simple and low-cost extraction protocol followed by a liquid chromatographic–tandem mass spectrometry method was developed for the simultaneous detection of nine sulfonamides in whole milk. The method was validated at the maximum residue limits established by European legislation. The limits of quantification obtained for most sulfonamides were between 12.5 and 25 μg kg−1, detection capabilities ranged from 116 to 145 μg kg−1, and recoveries, at 100 μg kg−1, were greater than 89 ± 12.5%. The method was employed to analyse 100 raw whole bovine milk samples collected from dairy farms in the northwest region of Spain. All of the samples were found to be compliant, but two were positive; one for sulfadiazine and the other for sulfamethoxipyridazine.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-12-01
2013
2013-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10347/45563
url https://hdl.handle.net/10347/45563
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
instname:Universidad de Santiago de Compostela (USC)
instname_str Universidad de Santiago de Compostela (USC)
reponame_str Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
collection Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
repository.name.fl_str_mv
repository.mail.fl_str_mv
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