Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage

Bisphenol A (BPA; 4,4’-(propane-2,2-diyl)diphenol) has been shown to act as an obesogen and to disrupt lipid metabolism in zebrafish eleutheroembryos (ZE). To characterize the consequences of this disruption, we performed a detailed lipidomic study using ZE exposed to different BPA concentrations (0...

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Autores: Martínez, Rubén, Navarro-Martín, Laia, van Antro, Morgane, Fuertes, Inmaculada, Casado, Marta, Barata Martí, Carlos, Piña, Benjamín
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/197531
Acesso em linha:http://hdl.handle.net/10261/197531
Access Level:acceso abierto
Palavra-chave:Mass spectrometry
Adverse outcome pathway
Endocrine disruption
Phenols
Thin layer chromatography
Mode of action
Yolk sac malabsorption
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spelling Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stageMartínez, RubénNavarro-Martín, Laiavan Antro, MorganeFuertes, InmaculadaCasado, MartaBarata Martí, CarlosPiña, BenjamínMass spectrometryAdverse outcome pathwayEndocrine disruptionPhenolsThin layer chromatographyMode of actionYolk sac malabsorptionBisphenol A (BPA; 4,4’-(propane-2,2-diyl)diphenol) has been shown to act as an obesogen and to disrupt lipid metabolism in zebrafish eleutheroembryos (ZE). To characterize the consequences of this disruption, we performed a detailed lipidomic study using ZE exposed to different BPA concentrations (0, 4, 6 and 8 mg/L of BPA) from day 2 to up to day 6 post fertilization (dpf). Total lipids at 4, 5 and 6 dpf were extracted by Folch method and analyzed by high-performance thin layer chromatography (HPTLC) as wide-range preliminary screening. Selected conditions (0 and 6 mg/L of BPA) were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC-TOFMS). BPA exposed ZE exhibited increased amounts of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), regarding the control group. Analysis of time- and BPA exposure-related patterns of specific lipid species showed a clear influence of unsaturation degree (mostly in DG and PC) and/or fatty acid chain length (mostly in TG and PC derivatives) on their response to the presence of BPA. A decreased yolk-sac and energy consumption in exposed individuals appeared as the main reason for the observed BPA-driven effects. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests a disruption of different signalling pathways by BPA that starts at very low BPAconcentrations, whose effects propagate across different organization levels, and that cannot be only explained by the relatively weak estrogenic effect of BPA. © 2019 Elsevier LtdThis work was supported by grants from the Spanish Ministry of Economy and Competitiveness (CTQ2014-56777-R) and the Spanish Ministry of Science, Innovation and University (RTI2018-096175-B-I00). RM was supported by a FPU predoctoral fellow from the Spanish Ministry of Education, Culture and Sport (ref. FPU15/03332). IF was supported by a FPI predoctoral fellow from the Spanish Ministry of Economy and Competitiveness (FPI-MICINN BES-2015-075023). We thank Juan Carlos Navarro and Josefina Casas for his valuable advice and expertise in the optimization of the thin-layer chromatography technique and liquid chromatography/mass spectrometry, respectively.Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Martínez, Rubén [0000-0001-9965-7042]Navarro-Martín, Laia [0000-0001-6554-8833]Fuertes, Inmaculada [0000-0001-8415-7993]Barata Martí, Carlos [0000-0002-3360-0729]PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/197531reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-Rhttp://hdl.handle.net/10261/192701https://doi.org/10.1016/j.chemosphere.2019.125704Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1975312026-05-22T06:33:51Z
dc.title.none.fl_str_mv Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
title Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
spellingShingle Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
Martínez, Rubén
Mass spectrometry
Adverse outcome pathway
Endocrine disruption
Phenols
Thin layer chromatography
Mode of action
Yolk sac malabsorption
title_short Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
title_full Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
title_fullStr Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
title_full_unstemmed Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
title_sort Changes in lipid profiles induced by bisphenol A (BPA) in zebrafish eleutheroembryos during the yolk sac absorption stage
dc.creator.none.fl_str_mv Martínez, Rubén
Navarro-Martín, Laia
van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author Martínez, Rubén
author_facet Martínez, Rubén
Navarro-Martín, Laia
van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author_role author
author2 Navarro-Martín, Laia
van Antro, Morgane
Fuertes, Inmaculada
Casado, Marta
Barata Martí, Carlos
Piña, Benjamín
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Martínez, Rubén [0000-0001-9965-7042]
Navarro-Martín, Laia [0000-0001-6554-8833]
Fuertes, Inmaculada [0000-0001-8415-7993]
Barata Martí, Carlos [0000-0002-3360-0729]
PIÑA CAPO, BENJAMIN CAYETANO [0000-0001-9216-2768]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mass spectrometry
Adverse outcome pathway
Endocrine disruption
Phenols
Thin layer chromatography
Mode of action
Yolk sac malabsorption
topic Mass spectrometry
Adverse outcome pathway
Endocrine disruption
Phenols
Thin layer chromatography
Mode of action
Yolk sac malabsorption
description Bisphenol A (BPA; 4,4’-(propane-2,2-diyl)diphenol) has been shown to act as an obesogen and to disrupt lipid metabolism in zebrafish eleutheroembryos (ZE). To characterize the consequences of this disruption, we performed a detailed lipidomic study using ZE exposed to different BPA concentrations (0, 4, 6 and 8 mg/L of BPA) from day 2 to up to day 6 post fertilization (dpf). Total lipids at 4, 5 and 6 dpf were extracted by Folch method and analyzed by high-performance thin layer chromatography (HPTLC) as wide-range preliminary screening. Selected conditions (0 and 6 mg/L of BPA) were used to obtain a high-quality lipid profile using ultra high-performance liquid chromatography/time-of-flight mass spectrometry (UHPLC-TOFMS). BPA exposed ZE exhibited increased amounts of triglycerides (TG), diglycerides (DG), phosphatidylcholines (PC) and phosphatidylinositols (PI), regarding the control group. Analysis of time- and BPA exposure-related patterns of specific lipid species showed a clear influence of unsaturation degree (mostly in DG and PC) and/or fatty acid chain length (mostly in TG and PC derivatives) on their response to the presence of BPA. A decreased yolk-sac and energy consumption in exposed individuals appeared as the main reason for the observed BPA-driven effects. Integration of these results with previous morphological, biochemical, transcriptomic, metabolomic and behavioral data suggests a disruption of different signalling pathways by BPA that starts at very low BPAconcentrations, whose effects propagate across different organization levels, and that cannot be only explained by the relatively weak estrogenic effect of BPA. © 2019 Elsevier Ltd
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/197531
url http://hdl.handle.net/10261/197531
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2014-56777-R
http://hdl.handle.net/10261/192701
https://doi.org/10.1016/j.chemosphere.2019.125704
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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