Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique

Bioanalytical methods, in particular electrochemical biosensors, are increasingly used in different industrial sectors due to their simplicity, low cost, and fast response. However, to be able to reliably use this type of device, it is necessary to undertake in-depth evaluation of their fundamental...

Descripción completa

Detalles Bibliográficos
Autores: Artigues Cladera, Margalida, Gilabert-Porres, Joan, Texidó Bartes, Robert, Borrós, Salvador, Abella, Jordi, Colominas Fuster, Sergi
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.14342/3944
Acceso en línea:http://hdl.handle.net/20.500.14342/3944
https://doi.org/10.3390/s21124185
Access Level:acceso abierto
Palabra clave:Bioanalytical methods
Electrochemical biosensor
Hydroxyethyl methacrylate (HEMA)
Pentafluorophenyl methacrylate (PFM)
Glucose oxidase (GOx)
6
id ES_da9257e91d00fbb49c43512873e42e5e
oai_identifier_str oai:recercat.cat:20.500.14342/3944
network_acronym_str ES
network_name_str España
repository_id_str
spelling Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization techniqueArtigues Cladera, MargalidaGilabert-Porres, JoanTexidó Bartes, RobertBorrós, SalvadorAbella, JordiColominas Fuster, SergiBioanalytical methodsElectrochemical biosensorHydroxyethyl methacrylate (HEMA)Pentafluorophenyl methacrylate (PFM)Glucose oxidase (GOx)6Bioanalytical methods, in particular electrochemical biosensors, are increasingly used in different industrial sectors due to their simplicity, low cost, and fast response. However, to be able to reliably use this type of device, it is necessary to undertake in-depth evaluation of their fundamental analytical parameters. In this work, analytical parameters of an amperometric biosensor based on covalent immobilization of glucose oxidase (GOx) were evaluated. GOx was immobilized using plasma-grafted pentafluorophenyl methacrylate (pgPFM) as an anchor onto a tailored HEMA-co-EGDA hydrogel that coats a titanium dioxide nanotubes array (TiO2NTAs). Finally, chitosan was used to protect the enzyme molecules. The biosensor offered outstanding analytical parameters: repeatability (RSD = 1.7%), reproducibility (RSD = 1.3%), accuracy (deviation = 4.8%), and robustness (RSD = 2.4%). In addition, the Ti/TiO2NTAs/ppHEMA-co-EGDA/pgPFM/GOx/Chitosan biosensor showed good long-term stability; after 20 days, it retained 89% of its initial sensitivity. Finally, glucose concentrations of different food samples were measured and compared using an official standard method (HPLC). Deviation was lower than 10% in all measured samples. Therefore, the developed biosensor can be considered to be a reliable analytical tool for quantification measurements.info:eu-repo/semantics/publishedVersionMDPIUniversitat Ramon Llull. IQS2021info:eu-repo/semantics/article15 p.http://hdl.handle.net/20.500.14342/3944https://doi.org/10.3390/s21124185reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésSensors© L'autor/aAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.14342/39442026-05-29T05:05:01Z
dc.title.none.fl_str_mv Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
title Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
spellingShingle Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
Artigues Cladera, Margalida
Bioanalytical methods
Electrochemical biosensor
Hydroxyethyl methacrylate (HEMA)
Pentafluorophenyl methacrylate (PFM)
Glucose oxidase (GOx)
6
title_short Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
title_full Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
title_fullStr Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
title_full_unstemmed Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
title_sort Analytical parameters of a novel glucose biosensor based on grafted PFM as a covalent immobilization technique
dc.creator.none.fl_str_mv Artigues Cladera, Margalida
Gilabert-Porres, Joan
Texidó Bartes, Robert
Borrós, Salvador
Abella, Jordi
Colominas Fuster, Sergi
author Artigues Cladera, Margalida
author_facet Artigues Cladera, Margalida
Gilabert-Porres, Joan
Texidó Bartes, Robert
Borrós, Salvador
Abella, Jordi
Colominas Fuster, Sergi
author_role author
author2 Gilabert-Porres, Joan
Texidó Bartes, Robert
Borrós, Salvador
Abella, Jordi
Colominas Fuster, Sergi
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Ramon Llull. IQS
dc.subject.none.fl_str_mv Bioanalytical methods
Electrochemical biosensor
Hydroxyethyl methacrylate (HEMA)
Pentafluorophenyl methacrylate (PFM)
Glucose oxidase (GOx)
6
topic Bioanalytical methods
Electrochemical biosensor
Hydroxyethyl methacrylate (HEMA)
Pentafluorophenyl methacrylate (PFM)
Glucose oxidase (GOx)
6
description Bioanalytical methods, in particular electrochemical biosensors, are increasingly used in different industrial sectors due to their simplicity, low cost, and fast response. However, to be able to reliably use this type of device, it is necessary to undertake in-depth evaluation of their fundamental analytical parameters. In this work, analytical parameters of an amperometric biosensor based on covalent immobilization of glucose oxidase (GOx) were evaluated. GOx was immobilized using plasma-grafted pentafluorophenyl methacrylate (pgPFM) as an anchor onto a tailored HEMA-co-EGDA hydrogel that coats a titanium dioxide nanotubes array (TiO2NTAs). Finally, chitosan was used to protect the enzyme molecules. The biosensor offered outstanding analytical parameters: repeatability (RSD = 1.7%), reproducibility (RSD = 1.3%), accuracy (deviation = 4.8%), and robustness (RSD = 2.4%). In addition, the Ti/TiO2NTAs/ppHEMA-co-EGDA/pgPFM/GOx/Chitosan biosensor showed good long-term stability; after 20 days, it retained 89% of its initial sensitivity. Finally, glucose concentrations of different food samples were measured and compared using an official standard method (HPLC). Deviation was lower than 10% in all measured samples. Therefore, the developed biosensor can be considered to be a reliable analytical tool for quantification measurements.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.14342/3944
https://doi.org/10.3390/s21124185
url http://hdl.handle.net/20.500.14342/3944
https://doi.org/10.3390/s21124185
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sensors
dc.rights.none.fl_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © L'autor/a
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 15 p.
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421590919249920
score 15,81155

Similares en LA Referencia