LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor

[EN] LAL regulators (Large ATP-binding regulators of the LuxR family) constitute a poorly studied family of transcriptional regulators. Several regulators of this class have been identified in antibiotic and other secondary metabolite gene clusters from actinomycetes, thus they have been considered...

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Autores: Martínez Guerra, Susana, Rodríguez García, Antonio, Santos Aberturas, Javier, Morgado Vicente, Cláudia Sofia, Diez Payero, Tamara, Martín Martín, Juan Francisco, Aparicio Fernández, Jesús Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/17977
Acceso en línea:https://journals.plos.org/plosone/article/metrics?id=10.1371/journal.pone.0031475
https://hdl.handle.net/10612/17977
Access Level:acceso abierto
Palabra clave:Biología
Streptomyces coelicolor
Antibotics biosynthesis regulation
Phosphate regulation
LAL regulators
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
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spelling LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolorMartínez Guerra, SusanaRodríguez García, AntonioSantos Aberturas, JavierMorgado Vicente, Cláudia SofiaDiez Payero, TamaraMartín Martín, Juan FranciscoAparicio Fernández, Jesús ManuelBiologíaStreptomyces coelicolorAntibotics biosynthesis regulationPhosphate regulationLAL regulators2415.01 Biología Molecular de Microorganismos2414.02 Fisiología Bacteriana[EN] LAL regulators (Large ATP-binding regulators of the LuxR family) constitute a poorly studied family of transcriptional regulators. Several regulators of this class have been identified in antibiotic and other secondary metabolite gene clusters from actinomycetes, thus they have been considered pathway-specific regulators. In this study we have obtained two disruption mutants of LAL genes from S. coelicolor (Δ0877 and Δ7173). Both mutants were deficient in the production of the polyketide antibiotic actinorhodin, and antibiotic production was restored upon gene complementation of the mutants. The use of whole-genome DNA microarrays and quantitative PCRs enabled the analysis of the transcriptome of both mutants in comparison with the wild type. Our results indicate that the LAL regulators under study act globally affecting various cellular processes, and amongst them the phosphate starvation response and the biosynthesis of the blue-pigmented antibiotic actinorhodin. Both regulators act as negative modulators of the expression of the two-component phoRP system and as positive regulators of actinorhodin biosynthesis. To our knowledge this is the first characterization of LAL regulators with wide implications in Streptomyces metabolismSIThis work was supported by the Spanish Ministerio de Ciencia e Innovación (BIO2007-67585 and BIO2010-19911 to JFA); and the Junta de Castilla y León (Grupo de Excelencia GR117). FPU fellowships of the Ministerio de Ciencia e Innovación (AP2005-3644 to JSA, AP2007-02055 to T.D.P.); a fellowship from the University of León (to SMG); and a contract from the Junta de Castilla y León (E-24-2009-0053914 to CMV). The funders had no role in study design, data collection and analysis, decision to publish, or presentation of the manuscript.Public Library of ScienceMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://journals.plos.org/plosone/article/metrics?id=10.1371/journal.pone.0031475https://hdl.handle.net/10612/17977reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad Rey Juan CarlosInglésinfo:eu-repo/grantAgreement/MICINN//BIO2007-67585info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/179772026-06-24T12:43:27Z
dc.title.none.fl_str_mv LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
title LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
spellingShingle LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
Martínez Guerra, Susana
Biología
Streptomyces coelicolor
Antibotics biosynthesis regulation
Phosphate regulation
LAL regulators
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
title_short LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
title_full LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
title_fullStr LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
title_full_unstemmed LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
title_sort LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
dc.creator.none.fl_str_mv Martínez Guerra, Susana
Rodríguez García, Antonio
Santos Aberturas, Javier
Morgado Vicente, Cláudia Sofia
Diez Payero, Tamara
Martín Martín, Juan Francisco
Aparicio Fernández, Jesús Manuel
author Martínez Guerra, Susana
author_facet Martínez Guerra, Susana
Rodríguez García, Antonio
Santos Aberturas, Javier
Morgado Vicente, Cláudia Sofia
Diez Payero, Tamara
Martín Martín, Juan Francisco
Aparicio Fernández, Jesús Manuel
author_role author
author2 Rodríguez García, Antonio
Santos Aberturas, Javier
Morgado Vicente, Cláudia Sofia
Diez Payero, Tamara
Martín Martín, Juan Francisco
Aparicio Fernández, Jesús Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Streptomyces coelicolor
Antibotics biosynthesis regulation
Phosphate regulation
LAL regulators
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
topic Biología
Streptomyces coelicolor
Antibotics biosynthesis regulation
Phosphate regulation
LAL regulators
2415.01 Biología Molecular de Microorganismos
2414.02 Fisiología Bacteriana
description [EN] LAL regulators (Large ATP-binding regulators of the LuxR family) constitute a poorly studied family of transcriptional regulators. Several regulators of this class have been identified in antibiotic and other secondary metabolite gene clusters from actinomycetes, thus they have been considered pathway-specific regulators. In this study we have obtained two disruption mutants of LAL genes from S. coelicolor (Δ0877 and Δ7173). Both mutants were deficient in the production of the polyketide antibiotic actinorhodin, and antibiotic production was restored upon gene complementation of the mutants. The use of whole-genome DNA microarrays and quantitative PCRs enabled the analysis of the transcriptome of both mutants in comparison with the wild type. Our results indicate that the LAL regulators under study act globally affecting various cellular processes, and amongst them the phosphate starvation response and the biosynthesis of the blue-pigmented antibiotic actinorhodin. Both regulators act as negative modulators of the expression of the two-component phoRP system and as positive regulators of actinorhodin biosynthesis. To our knowledge this is the first characterization of LAL regulators with wide implications in Streptomyces metabolism
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article/metrics?id=10.1371/journal.pone.0031475
https://hdl.handle.net/10612/17977
url https://journals.plos.org/plosone/article/metrics?id=10.1371/journal.pone.0031475
https://hdl.handle.net/10612/17977
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//BIO2007-67585
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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