The dynamic architecture of the metabolic switch in Streptomyces coelicolor

[EN] Background: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high...

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Detalles Bibliográficos
Autores: Nieselt, Kay, Battke, Florian, Herbig, Alexander, Bruheim, Per, Wentzel, Alexander, Jakobsen, Øyvind M, Sletta, Håvard, Alam, Mohammad T, Merlo, Maria E, Moore, Jonathan, Omara, Walid AM, Morrissey, Edward R, Juarez-Hermosillo, Miguel A, Rodríguez García, Antonio, Nentwich, Merle, Thomas, Louise, Iqbal, Mudassar, Legaie, Roxane, Gaze, William H, Challis, Gregory L, Jansen, Ritsert C, Dijkhuizen, Lubbert, Rand, David A, Wild, David L, Bonin, Michael, Reuther, Jens, Wohlleben, Wolfgang, Smith, Margaret CM, Burroughs, Nigel J, Martín Martín, Juan Francisco, Hodgson, David A, Takano, Eriko, Breitling, Rainer, Ellingsen, Trond E, Wellington, Elizabeth MH
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2010
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/18079
Acceso en línea:https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-11-10
https://hdl.handle.net/10612/18079
Access Level:acceso abierto
Palabra clave:Biología
Streptomyces coelicolor
Phosphate regulation
Transcriptomics
Antibotics biosynthesis regulation
Metabolic switch
2414.02 Fisiología Bacteriana
2415.01 Biología Molecular de Microorganismos
Descripción
Sumario:[EN] Background: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples.Results: Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis.Conclusions: Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting

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