Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

18 pags, 11 figs, 5 tabs

Bibliographic Details
Authors:	Pandey, Suraj, Calvey, George, Katz, Andrea M., Malla, Tek Narsingh, Koua, Faisal H. M., Martín-García, José M., Poudyal, Ishwor, Yang, Jay-How, Vakili, Mohammad, Yefanov, Oleksandr, Zielinski, Kara A., Bajt, Sasa, Awel, Salah, Doerner, Katarina, Frank, Matthias, Gelisio, Luca, Jernigan, Rebecca, Kirkwood, Henry, Kloos, Marco, Koliyadu, Jayanath, Mariani, Valerio, Miller, Mitchell D., Mills, Grant, Nelson, Garrett, Olmos, Jose L., Sadri, Alireza, Sato, Tokushi, Tolstikova, Alexandra, Xu, Weijun, Ourmazd, Abbas, Spence, John C. H., Schwander, Peter, Barty, Anton, Chapman, Henry N., Fromme, Petra, Mancuso, Adrian P., Phillips, George N., Bean, Richard, Pollack, Lois, Schmidt, Marius
Format:	article
Status:	Published version
Publication Date:	2021
Country:	España
Institution:	Consejo Superior de Investigaciones Científicas (CSIC)
Repository:	DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:	oai:digital.csic.es:10261/258729
Online Access:	http://hdl.handle.net/10261/258729 https://api.elsevier.com/content/abstract/scopus_id/85118931472
Access Level:	Open access
Keyword:	European X-ray Free-Electron Laser X-ray crystallography Antibiotic resistance Ceftriaxone Drug discovery Enzyme kinetics Enzyme mechanisms Irreversible inhibition Megahertz pulse-repetition rate Mix-and-inject serial crystallography Protein structure determination Serial femtosecond crystallography Substrate diffusion in crystals Sulbactam β-lactamases

Description
Summary:	18 pags, 11 figs, 5 tabs