Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal

Chemical products still represent the most common form of controlling crop pests and diseases. However, their extensive use has led to the selection of resistances. This makes the finding of new solutions paramount to countering the economic losses that pests and diseases represent in modern agricul...

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Authors: Unzue Pozas, Argiñe, Caballero Sánchez, Carlos, Villanueva, Maite, Fernández González, Ana Beatriz, Caballero Murillo, Primitivo
Format: article
Status:Published version
Publication Date:2022
Country:España
Institution:Universidad Pública de Navarra
Repository:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/45095
Online Access:https://hdl.handle.net/2454/45095
Access Level:Open access
Keyword:Bacillus thuringiensis
Bioassays
Coleoptera
Mite
Multifunctional
Phytopathogenic fungi
Plant-parasitic nematode
Toxicity
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spelling Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystalUnzue Pozas, ArgiñeCaballero Sánchez, CarlosVillanueva, MaiteFernández González, Ana BeatrizCaballero Murillo, PrimitivoBacillus thuringiensisBioassaysColeopteraMiteMultifunctionalPhytopathogenic fungiPlant-parasitic nematodeToxicityChemical products still represent the most common form of controlling crop pests and diseases. However, their extensive use has led to the selection of resistances. This makes the finding of new solutions paramount to countering the economic losses that pests and diseases represent in modern agriculture. Bacillus thuringiensis (Bt) is one of the most reliable alternatives to chemical-based solutions. In this study, we aimed to further expand the global applicability of Bt strains beyond their spores and crystals. To this end, we selected a new Bt strain (BST-122) with relevant toxicity factors and tested its activity against species belonging to different phyla. The spore and crystal mixture showed toxicity to coleopterans. Additionally, a novel Cry5-like protein proved active against the two-spotted spider mite. In vivo and plant assays revealed significant control of the parasitic nematode, Meloidogyne incognita. Surprisingly, our data indicated that the nematocidal determinants may be secreted. When evaluated against phytopathogenic fungi, the strain seemed to decelerate their growth. Overall, our research has highlighted the potential of Bt strains, expanding their use beyond the confinements of spores and crystals. However, further studies are required to pinpoint the factors responsible for the wide host range properties of the BST-122 strain.A. Unzue Doctorados Industriales predoctoral contract was cofounded by Gobierno de Navarra (0011-1408-2017-000027) and Bioinsectis SL. A. B. Fernández contract was cofounded by the Program Torres Quevedo (Ministerio de Ciencia, Innovación y Universidades, Gobierno de España) (PTQ-2018-010091) and Bioinsectis SL.MDPIInstitute for Multidisciplinary Research in Applied Biology - IMAB2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/45095reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MICIU//PTQ-2018-010091info:eu-repo/grantAgreement/Gobierno de Navarra//0011-1408-2017-000027© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/450952026-06-17T12:41:47Z
dc.title.none.fl_str_mv Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
title Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
spellingShingle Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
Unzue Pozas, Argiñe
Bacillus thuringiensis
Bioassays
Coleoptera
Mite
Multifunctional
Phytopathogenic fungi
Plant-parasitic nematode
Toxicity
title_short Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
title_full Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
title_fullStr Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
title_full_unstemmed Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
title_sort Multifunctional properties of a bacillus thuringiensis strain (BST-122): beyond the parasporal crystal
dc.creator.none.fl_str_mv Unzue Pozas, Argiñe
Caballero Sánchez, Carlos
Villanueva, Maite
Fernández González, Ana Beatriz
Caballero Murillo, Primitivo
author Unzue Pozas, Argiñe
author_facet Unzue Pozas, Argiñe
Caballero Sánchez, Carlos
Villanueva, Maite
Fernández González, Ana Beatriz
Caballero Murillo, Primitivo
author_role author
author2 Caballero Sánchez, Carlos
Villanueva, Maite
Fernández González, Ana Beatriz
Caballero Murillo, Primitivo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Institute for Multidisciplinary Research in Applied Biology - IMAB
dc.subject.none.fl_str_mv Bacillus thuringiensis
Bioassays
Coleoptera
Mite
Multifunctional
Phytopathogenic fungi
Plant-parasitic nematode
Toxicity
topic Bacillus thuringiensis
Bioassays
Coleoptera
Mite
Multifunctional
Phytopathogenic fungi
Plant-parasitic nematode
Toxicity
description Chemical products still represent the most common form of controlling crop pests and diseases. However, their extensive use has led to the selection of resistances. This makes the finding of new solutions paramount to countering the economic losses that pests and diseases represent in modern agriculture. Bacillus thuringiensis (Bt) is one of the most reliable alternatives to chemical-based solutions. In this study, we aimed to further expand the global applicability of Bt strains beyond their spores and crystals. To this end, we selected a new Bt strain (BST-122) with relevant toxicity factors and tested its activity against species belonging to different phyla. The spore and crystal mixture showed toxicity to coleopterans. Additionally, a novel Cry5-like protein proved active against the two-spotted spider mite. In vivo and plant assays revealed significant control of the parasitic nematode, Meloidogyne incognita. Surprisingly, our data indicated that the nematocidal determinants may be secreted. When evaluated against phytopathogenic fungi, the strain seemed to decelerate their growth. Overall, our research has highlighted the potential of Bt strains, expanding their use beyond the confinements of spores and crystals. However, further studies are required to pinpoint the factors responsible for the wide host range properties of the BST-122 strain.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/45095
url https://hdl.handle.net/2454/45095
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICIU//PTQ-2018-010091
info:eu-repo/grantAgreement/Gobierno de Navarra//0011-1408-2017-000027
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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instname_str Universidad Pública de Navarra
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