Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli

[EN] he use of probiotic lactobacilli has been proposed as a strategy to mitigate damage associ- ated with exposure to toxic metals. Their protective effect against cationic metal ions, such as those of mercury or lead, is believed to stem from their chelating and accumulating potential. However, th...

Descripción completa

Detalles Bibliográficos
Autores: Corrales-Benedetti, Daniela, Alcántara, Cristina, Clemente, María Jesús, Vélez, Dinoraz, Devesa, Vicenta, Monedero, Vicente, Zúñiga, Manuel
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/220353
Acceso en línea:https://riunet.upv.es/handle/10251/220353
Access Level:acceso abierto
Palabra clave:Lactiplantibacillus plantarum
Lacticaseibacillus paracasei
Arsenate
Arsenite
Phosphate transporters
Two-component system
PhoP
id ES_d985bbb035dbefda9fdd55fa49d2047a
oai_identifier_str oai:riunet.upv.es:10251/220353
network_acronym_str ES
network_name_str España
repository_id_str
spelling Phosphate Uptake and Its Relation to Arsenic Toxicity in LactobacilliCorrales-Benedetti, DanielaAlcántara, CristinaClemente, María JesúsVélez, DinorazDevesa, VicentaMonedero, VicenteZúñiga, ManuelLactiplantibacillus plantarumLacticaseibacillus paracaseiArsenateArsenitePhosphate transportersTwo-component systemPhoP[EN] he use of probiotic lactobacilli has been proposed as a strategy to mitigate damage associ- ated with exposure to toxic metals. Their protective effect against cationic metal ions, such as those of mercury or lead, is believed to stem from their chelating and accumulating potential. However, their retention of anionic toxic metalloids, such as inorganic arsenic, is generally low. Through the construction of mutants in phosphate transporter genes (pst) in Lactiplantibacillus plantarum and Lacticaseibacillus paracasei strains, coupled with arsenate [As(V)] uptake and toxicity assays, we deter- mined that the incorporation of As(V), which structurally resembles phosphate, is likely facilitated by phosphate transporters. Surprisingly, inactivation in Lc. paracasei of PhoP, the transcriptional regulator of the two-component system PhoPR, a signal transducer involved in phosphate sensing, led to an increased resistance to arsenite [As(III)]. In comparison to the wild type, the phoP strain exhibited no differences in the ability to retain As(III), and there were no observed changes in the oxidation of As(III) to the less toxic As(V). These results reinforce the idea that specific transport, and not unspecific cell retention, plays a role in As(V) biosorption by lactobacilli, while they reveal an unexpected phenotype for the lack of the pleiotropic regulator PhoP.This research was funded by the Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación (MCIU/AEI/10.13039/501100011033) from Spain, grant number RTI2018-098071-B-I00, and it was co-funded by the European Commission through the European Regional Development Fund (Multiregional operative program for Spain 2014 2020) and by Generalitat Valenciana, grant number AICO/2019/008. The Accreditation as Center of Excellence Severo Ochoa CEX2021-001189-S funded by MCIU/AEI is also fully acknowledged.MDPI AGGeneralitat ValencianaAgencia Estatal de InvestigaciónRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/220353reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098071-B-I00 ESTRATEGIAS PARA MITIGAR LA TOXICIDAD ASOCIADA A LA EXPOSICION CRONICA A ARSENICO O MERCURIO A TRAVES DE LA DIETAGeneralitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F008Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2021-001189-Sopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2203532026-06-13T07:49:27Z
dc.title.none.fl_str_mv Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
title Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
spellingShingle Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
Corrales-Benedetti, Daniela
Lactiplantibacillus plantarum
Lacticaseibacillus paracasei
Arsenate
Arsenite
Phosphate transporters
Two-component system
PhoP
title_short Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
title_full Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
title_fullStr Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
title_full_unstemmed Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
title_sort Phosphate Uptake and Its Relation to Arsenic Toxicity in Lactobacilli
dc.creator.none.fl_str_mv Corrales-Benedetti, Daniela
Alcántara, Cristina
Clemente, María Jesús
Vélez, Dinoraz
Devesa, Vicenta
Monedero, Vicente
Zúñiga, Manuel
author Corrales-Benedetti, Daniela
author_facet Corrales-Benedetti, Daniela
Alcántara, Cristina
Clemente, María Jesús
Vélez, Dinoraz
Devesa, Vicenta
Monedero, Vicente
Zúñiga, Manuel
author_role author
author2 Alcántara, Cristina
Clemente, María Jesús
Vélez, Dinoraz
Devesa, Vicenta
Monedero, Vicente
Zúñiga, Manuel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Generalitat Valenciana
Agencia Estatal de Investigación
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Lactiplantibacillus plantarum
Lacticaseibacillus paracasei
Arsenate
Arsenite
Phosphate transporters
Two-component system
PhoP
topic Lactiplantibacillus plantarum
Lacticaseibacillus paracasei
Arsenate
Arsenite
Phosphate transporters
Two-component system
PhoP
description [EN] he use of probiotic lactobacilli has been proposed as a strategy to mitigate damage associ- ated with exposure to toxic metals. Their protective effect against cationic metal ions, such as those of mercury or lead, is believed to stem from their chelating and accumulating potential. However, their retention of anionic toxic metalloids, such as inorganic arsenic, is generally low. Through the construction of mutants in phosphate transporter genes (pst) in Lactiplantibacillus plantarum and Lacticaseibacillus paracasei strains, coupled with arsenate [As(V)] uptake and toxicity assays, we deter- mined that the incorporation of As(V), which structurally resembles phosphate, is likely facilitated by phosphate transporters. Surprisingly, inactivation in Lc. paracasei of PhoP, the transcriptional regulator of the two-component system PhoPR, a signal transducer involved in phosphate sensing, led to an increased resistance to arsenite [As(III)]. In comparison to the wild type, the phoP strain exhibited no differences in the ability to retain As(III), and there were no observed changes in the oxidation of As(III) to the less toxic As(V). These results reinforce the idea that specific transport, and not unspecific cell retention, plays a role in As(V) biosorption by lactobacilli, while they reveal an unexpected phenotype for the lack of the pleiotropic regulator PhoP.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/220353
url https://riunet.upv.es/handle/10251/220353
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-098071-B-I00 ESTRATEGIAS PARA MITIGAR LA TOXICIDAD ASOCIADA A LA EXPOSICION CRONICA A ARSENICO O MERCURIO A TRAVES DE LA DIETA
Generalitat Valenciana https://doi.org/10.13039/501100003359 AICO%2F2019%2F008
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 CEX2021-001189-S
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421395051544576
score 15.812429

Similares en LA Referencia