Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes

In this work we report on the characterization and biological functionalization of 2D MoS flakes, epitaxially grown on sapphire, to develop an optical biosensor for the breast cancer biomarker miRNA21. The MoS flakes were modified with a thiolated DNA probe complementary to the target biomarker. Bas...

ver descrição completa

Detalhes bibliográficos
Autores: Catalán-Gómez, Sergio, Briones, María, Cortijo-Campos, Sandra, García-Mendiola, Tania, Andrés, Alicia de, Garg, Sourav, Kung, Patrick, Lorenzo, Encarnación, Pau, José Luis, Redondo-Cubero, Andrés
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/235459
Acesso em linha:http://hdl.handle.net/10261/235459
Access Level:acceso abierto
Palavra-chave:Optical biosensors
MoS2 monolayer
Breast cancer
Biomarkers
Photoluminescence
id ES_d95bea95cb013faa2ad6dc3564f3b6ea
oai_identifier_str oai:digital.csic.es:10261/235459
network_acronym_str ES
network_name_str España
repository_id_str
spelling Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakesCatalán-Gómez, SergioBriones, MaríaCortijo-Campos, SandraGarcía-Mendiola, TaniaAndrés, Alicia deGarg, SouravKung, PatrickLorenzo, EncarnaciónPau, José LuisRedondo-Cubero, AndrésOptical biosensorsMoS2 monolayerBreast cancerBiomarkersPhotoluminescenceIn this work we report on the characterization and biological functionalization of 2D MoS flakes, epitaxially grown on sapphire, to develop an optical biosensor for the breast cancer biomarker miRNA21. The MoS flakes were modified with a thiolated DNA probe complementary to the target biomarker. Based on the photoluminescence of MoS, the hybridization events were analyzed for the target (miRNA21c) and the control non-complementary sequence (miRNA21nc). A specific redshift was observed for the hybridization with miRNA21c, but not for the control, demonstrating the biomarker recognition via PL. The homogeneity of these MoS platforms was verified with microscopic maps. The detailed spectroscopic analysis of the spectra reveals changes in the trion to excitation ratio, being the redshift after the hybridization ascribed to both peaks. The results demonstrate the benefits of optical biosensors based on MoS monolayer for future commercial devices.The research is supported by the MINECO (CTQ2017-84309-C2-2-R, CTQ2017-84309-C2-1-R, ELECTROBIONET, RED2018-102412-T) and Comunidad de Madrid (TRANSNANOAVANSENS, P2018/NMT4349) projects. ARC acknowledges Ramón y Cajal program (under contract number RYC-2015-18047).Nature Publishing GroupMinisterio de Economía y Competitividad (España)Comunidad de MadridConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/235459reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84309-C2-2-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84309-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RED2018-102412-TP2018/NMT4349/TRANSNANOAVANSENSinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2015-18047http://dx.doi.org/10.1038/s41598-020-73029-9Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2354592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
title Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
spellingShingle Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
Catalán-Gómez, Sergio
Optical biosensors
MoS2 monolayer
Breast cancer
Biomarkers
Photoluminescence
title_short Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
title_full Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
title_fullStr Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
title_full_unstemmed Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
title_sort Breast cancer biomarker detection through the photoluminescence of epitaxial monolayer MoS2 flakes
dc.creator.none.fl_str_mv Catalán-Gómez, Sergio
Briones, María
Cortijo-Campos, Sandra
García-Mendiola, Tania
Andrés, Alicia de
Garg, Sourav
Kung, Patrick
Lorenzo, Encarnación
Pau, José Luis
Redondo-Cubero, Andrés
author Catalán-Gómez, Sergio
author_facet Catalán-Gómez, Sergio
Briones, María
Cortijo-Campos, Sandra
García-Mendiola, Tania
Andrés, Alicia de
Garg, Sourav
Kung, Patrick
Lorenzo, Encarnación
Pau, José Luis
Redondo-Cubero, Andrés
author_role author
author2 Briones, María
Cortijo-Campos, Sandra
García-Mendiola, Tania
Andrés, Alicia de
Garg, Sourav
Kung, Patrick
Lorenzo, Encarnación
Pau, José Luis
Redondo-Cubero, Andrés
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Optical biosensors
MoS2 monolayer
Breast cancer
Biomarkers
Photoluminescence
topic Optical biosensors
MoS2 monolayer
Breast cancer
Biomarkers
Photoluminescence
description In this work we report on the characterization and biological functionalization of 2D MoS flakes, epitaxially grown on sapphire, to develop an optical biosensor for the breast cancer biomarker miRNA21. The MoS flakes were modified with a thiolated DNA probe complementary to the target biomarker. Based on the photoluminescence of MoS, the hybridization events were analyzed for the target (miRNA21c) and the control non-complementary sequence (miRNA21nc). A specific redshift was observed for the hybridization with miRNA21c, but not for the control, demonstrating the biomarker recognition via PL. The homogeneity of these MoS platforms was verified with microscopic maps. The detailed spectroscopic analysis of the spectra reveals changes in the trion to excitation ratio, being the redshift after the hybridization ascribed to both peaks. The results demonstrate the benefits of optical biosensors based on MoS monolayer for future commercial devices.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/235459
url http://hdl.handle.net/10261/235459
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84309-C2-2-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-84309-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RED2018-102412-T
P2018/NMT4349/TRANSNANOAVANSENS
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC-2015-18047
http://dx.doi.org/10.1038/s41598-020-73029-9
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869421355713167360
score 15.811543

Registos relacionados