Evidence for cohesin sliding along budding yeast chromosomes.
[EN]The ring-shaped cohesin complex is thought to topologically hold sister chromatids together from their synthesis in S phase until chromosome segregation in mitosis. How cohesin stably binds to chromosomes for extended periods, without impeding other chromosomal processes that also require access...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2016 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/154883 |
| Acceso en línea: | http://hdl.handle.net/10366/154883 |
| Access Level: | acceso abierto |
| Palabra clave: | Cohesina Estabilidad del genoma Cohesión de cromáticas hermanas Saccharomyces cerevisiae Proteins DNA Replication Monosaccharide Transport Proteins Saccharomycetales Chromosomes Cysteine Synthase Chromosome Segregation HSP70 Heat-Shock Proteins Mitochondrial Proteins Transcriptional Activation Cell Cycle Proteins 24 Ciencias de la Vida proteínas mitocondriales proteínas de Saccharomyces cerevisiae cisteína sintasa proteínas de transporte de monosacáridos proteínas del ciclo celular activación transcripcional cromosomas replicación del ADN proteínas de choque térmico HSP70 segregación cromosómica |
| Sumario: | [EN]The ring-shaped cohesin complex is thought to topologically hold sister chromatids together from their synthesis in S phase until chromosome segregation in mitosis. How cohesin stably binds to chromosomes for extended periods, without impeding other chromosomal processes that also require access to the DNA, is poorly understood. Budding yeast cohesin is loaded onto DNA by the Scc2-Scc4 cohesin loader at centromeres and promoters of active genes, from where cohesin translocates to more permanent places of residence at transcription termination sites. Here we show that, at the GAL2 and MET17 loci, pre-existing cohesin is pushed downstream along the DNA in response to transcriptional gene activation, apparently without need for intermittent dissociation or reloading. We observe translocation intermediates and find that the distribution of most chromosomal cohesin is shaped by transcription. Our observations support a model in which cohesin is able to slide laterally along chromosomes while maintaining topological contact with DNA. In this way, stable cohesin binding to DNA and enduring sister chromatid cohesion become compatible with simultaneous underlying chromosomal activities, including but maybe not limited to transcription. |
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