Structure of the cohesin loader Scc2.
[EN]The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little i...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Estado: | Versão publicada |
| Data de publicação: | 2017 |
| País: | España |
| Recursos: | Universidad de Salamanca (USAL) |
| Repositório: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/154838 |
| Acesso em linha: | http://hdl.handle.net/10366/154838 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Cell Cycle Proteins Conserved Sequence Models, Molecular Protein Binding Protein Subunits Saccharomyces cerevisiae accharomyces cerevisiae Saccharomyces cerevisiae Proteins 24 Ciencias de la Vida secuencia conservada subunidades de proteínas proteínas de Saccharomyces cerevisiae unión proteica proteínas del ciclo celular |
| Resumo: | [EN]The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large 'hook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2-Scc4 complex. Finally, using recombinantly purified Scc2-Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2-Scc4 uses its modular structure to make multiple contacts with cohesin. |
|---|