Attomolar detection of hepatitis C virus core protein powered by molecular antenna-like effect in a graphene field-effect aptasensor

Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionali...

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Detalles Bibliográficos
Autores: Palacio, Irene, Moreno, Miguel, Náñez, Almudena, Purwidyantri, Agnes, Domingues, Telma, Cabral, Patricia D., Borme, Jérôme, Marciello, Marzia, Mendieta-Moreno, Jesús Ignacio, Torres-Vázquez, Beatriz, Martínez, José Ignacio, López, María Francisca, García-Hernández, Mar, Vázquez, Luis, Jelínek, Pavel, Alpuim, Pedro, Briones, Carlos, Martín-Gago, José Ángel
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/100703
Acceso en línea:https://hdl.handle.net/20.500.14352/100703
Access Level:acceso abierto
Palabra clave:615:54
615.31
Graphene
FET-biosensors
Aptamers
Covalent functionalization
HCV
Química
23 Química
Descripción
Sumario:Biosensors based on graphene field-effect transistors have become a promising tool for detecting a broad range of analytes. However, their performance is substantially affected by the functionalization protocol. In this work, we use a controlled in-vacuum physical method for the covalent functionalization of graphene to construct ultrasensitive aptamer-based biosensors (aptasensors) able to detect hepatitis C virus core protein. These devices are highly specific and robust, achieving attomolar detection of the viral protein in human blood plasma. Such an improved sensitivity is rationalized by theoretical calculations showing that induced polarization at the graphene interface, caused by the proximity of covalently bound molecular probe, modulates the charge balance at the graphene/aptamer interface. This charge balance causes a net shift of the Dirac cone providing enhanced sensitivity for the attomolar detection of the target proteins. Such an unexpected effect paves the way for using this kind of graphene-based functionalized platforms for ultrasensitive and real-time diagnostics of different diseases.

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