Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains

A total of 34 lactic acid bacteria isolates from 4 different Brazilian kefir grains were identified and characterized among a group of 150 isolates, using the ability to tolerate acidic pH and resistance to bile salts as restrictive criteria for probiotic potential. All isolates were identified by a...

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Autores: Leite, Analy M. O., Miguel, M. A. L., Peixoto, R. S., Ruas-Madiedo, Patricia, Paschoalin, V. M. F., Mayo Pérez, Baltasar, Delgado, Susana
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/143082
Acceso en línea:http://hdl.handle.net/10261/143082
Access Level:acceso abierto
Palabra clave:Kefir grain
Probiotic property
Lactobacilli
Lactic acid bacteria
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spelling Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grainsLeite, Analy M. O.Miguel, M. A. L.Peixoto, R. S.Ruas-Madiedo, PatriciaPaschoalin, V. M. F.Mayo Pérez, BaltasarDelgado, SusanaKefir grainProbiotic propertyLactobacilliLactic acid bacteriaA total of 34 lactic acid bacteria isolates from 4 different Brazilian kefir grains were identified and characterized among a group of 150 isolates, using the ability to tolerate acidic pH and resistance to bile salts as restrictive criteria for probiotic potential. All isolates were identified by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of representative amplicons. Eighteen isolates belonged to the species Leuconostoc mesenteroides, 11 to Lactococcus lactis (of which 8 belonged to subspecies cremoris and 3 to subspecies lactis), and 5 to Lactobacillus paracasei. To exclude replicates, a molecular typing analysis was performed by combining repetitive extragenic palindromic-PCR and random amplification of polymorphic DNA techniques. Considering a threshold of 90% similarity, 32 different strains were considered. All strains showed some antagonistic activity against 4 model food pathogens. In addition, 3 Lc. lactis strains and 1 Lb. paracasei produced bacteriocin-like inhibitory substances against at least 2 indicator organisms. Moreover, 1 Lc. lactis and 2 Lb. paracasei presented good total antioxidative activity. None of these strains showed undesirable enzymatic or hemolytic activities, while proving susceptible or intrinsically resistant to a series of clinically relevant antibiotics. The Lb. paracasei strain MRS59 showed a level of adhesion to human Caco-2 epithelial cells comparable with that observed for Lactobacillus rhamnosus GG. Taken together, these properties allow the MRS59 strain to be considered a promising probiotic candidate. © 2015 American Dairy Science Association.This study was supported by Fundação Carlos Chagas Filho de de Amparo à Pesquisa do Estado do Rio de Janeiro (Rio de Janeiro, Brazil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasília, Brazil) Foundation (process number PDEE 5019109), and funds from the Spanish Ministry of Science and Innovation (MICINN, Spain; reference AGL2011-24300 and AGL2012-33278). S. Delgado was supported by a research contract from MICINN under the Juan de la Cierva Program (reference JCI-2008-02391).Peer ReviewedElsevierFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)Ministerio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/143082reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3168/jds.2014-9265Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1430822026-05-22T06:33:51Z
dc.title.none.fl_str_mv Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
title Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
spellingShingle Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
Leite, Analy M. O.
Kefir grain
Probiotic property
Lactobacilli
Lactic acid bacteria
title_short Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
title_full Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
title_fullStr Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
title_full_unstemmed Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
title_sort Probiotic potential of selected lactic acid bacteria strains isolated from Brazilian kefir grains
dc.creator.none.fl_str_mv Leite, Analy M. O.
Miguel, M. A. L.
Peixoto, R. S.
Ruas-Madiedo, Patricia
Paschoalin, V. M. F.
Mayo Pérez, Baltasar
Delgado, Susana
author Leite, Analy M. O.
author_facet Leite, Analy M. O.
Miguel, M. A. L.
Peixoto, R. S.
Ruas-Madiedo, Patricia
Paschoalin, V. M. F.
Mayo Pérez, Baltasar
Delgado, Susana
author_role author
author2 Miguel, M. A. L.
Peixoto, R. S.
Ruas-Madiedo, Patricia
Paschoalin, V. M. F.
Mayo Pérez, Baltasar
Delgado, Susana
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil)
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Kefir grain
Probiotic property
Lactobacilli
Lactic acid bacteria
topic Kefir grain
Probiotic property
Lactobacilli
Lactic acid bacteria
description A total of 34 lactic acid bacteria isolates from 4 different Brazilian kefir grains were identified and characterized among a group of 150 isolates, using the ability to tolerate acidic pH and resistance to bile salts as restrictive criteria for probiotic potential. All isolates were identified by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of representative amplicons. Eighteen isolates belonged to the species Leuconostoc mesenteroides, 11 to Lactococcus lactis (of which 8 belonged to subspecies cremoris and 3 to subspecies lactis), and 5 to Lactobacillus paracasei. To exclude replicates, a molecular typing analysis was performed by combining repetitive extragenic palindromic-PCR and random amplification of polymorphic DNA techniques. Considering a threshold of 90% similarity, 32 different strains were considered. All strains showed some antagonistic activity against 4 model food pathogens. In addition, 3 Lc. lactis strains and 1 Lb. paracasei produced bacteriocin-like inhibitory substances against at least 2 indicator organisms. Moreover, 1 Lc. lactis and 2 Lb. paracasei presented good total antioxidative activity. None of these strains showed undesirable enzymatic or hemolytic activities, while proving susceptible or intrinsically resistant to a series of clinically relevant antibiotics. The Lb. paracasei strain MRS59 showed a level of adhesion to human Caco-2 epithelial cells comparable with that observed for Lactobacillus rhamnosus GG. Taken together, these properties allow the MRS59 strain to be considered a promising probiotic candidate. © 2015 American Dairy Science Association.
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/143082
url http://hdl.handle.net/10261/143082
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3168/jds.2014-9265
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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