Use of MALDI-TOF MS for Identification of Nontuberculous Mycobacterium Species Isolated from Clinical Specimens

The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various...

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Bibliographic Details
Authors: Mediavilla-Gradolph, María Concepción, De Toro-Peinado, Inmaculada, Bermúdez-Ruiz, María Pilar, García-Martínez, María de los Ángeles, Ortega-Torres, María, Montiel Quezel-Guerraz, Natalia, Palop-Borrás, Begoña
Format: article
Publication Date:2015
Country:España
Institution:Instituto de Salud Carlos III (ISCIII)
Repository:Repisalud
Language:English
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/17078
Online Access:http://hdl.handle.net/20.500.12105/17078
Access Level:Open access
Keyword:Humanos
Genotipo
Infecciones por micobacterias no tuberculosas
Micobacterias no tuberculosas
Espectrometría de masas con desorción-ionización láser asistida por matriz
Humans
Mycobacterium Infections, Nontuberculous
Nontuberculous Mycobacteria
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Genotype
Description
Summary:The aim of this study was to compare the results obtained for identification by MALDI-TOF of nontuberculous mycobacteria (NTM) isolated in clinical samples with those obtained by GenoType Mycobacterium CM/AS (common mycobacteria/additional species). A total of 66 Mycobacterium isolates from various clinical specimens (mainly respiratory) were tested in this study. They were identified using MALDI-TOF Bruker from strains isolated in Lowenstein, following the recommended protocol of heat inactivation and extraction, and were simultaneously analyzed through hybridization by GenoType Mycobacterium from liquid culture MGIT. Our results showed that identification by MALDI-TOF was correct in 98.4% (65/66) of NTM isolated in our clinical practice (M. avium, M. intracellulare, M. abscessus, M. chelonae, M. fortuitum, M. mucogenicum, M. kansasii, and M. scrofulaceum). MALDI-TOF was found to be an accurate, rapid, and cost-effective system for identification of mycobacteria species.

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