Localization of amidating enzymes (PAM) in rat gastrointestinal tract

Martinez, A. (Alfredo)|||/items/ce27c09e-454d-4645-986e-713760a3730d; Burrell, M.A. (María Ángela)|||/items/27fe7d6b-4d2b-4917-91af-68de4749f75b; Kuijk, M. (Marjolein)|||/items/975bacec-5070-4d15-bb4e-782d3beab9e3; Montuenga-Badia, L.M. (Luis M.)|||/items/4c999705-b2c9-45ac-ba13-3f18594ae596; Treston, A.M. (Anthony M.)|||/items/2a2177c8-e6d5-486c-841e-62a5423a037b; Cuttitta, F. (Frank)|||/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188; Polak, J.M. (Julia M.)|||/items/27bc2706-e444-4ece-99d6-b2bde0c966b4

Localization of amidating enzymes (PAM) in rat gastrointestinal tract

We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric a...

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Detalles Bibliográficos
Autores:	Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d, Burrell, M.A. (María Ángela)\|\|\|/items/27fe7d6b-4d2b-4917-91af-68de4749f75b, Kuijk, M. (Marjolein)\|\|\|/items/975bacec-5070-4d15-bb4e-782d3beab9e3, Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596, Treston, A.M. (Anthony M.)\|\|\|/items/2a2177c8-e6d5-486c-841e-62a5423a037b, Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188, Polak, J.M. (Julia M.)\|\|\|/items/27bc2706-e444-4ece-99d6-b2bde0c966b4
Tipo de recurso:	artículo
Fecha de publicación:	1993
País:	España
Institución:	Universidad de Navarra
Repositorio:	Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:	inglés
OAI Identifier:	oai:dadun.unav.edu:10171/20131
Acceso en línea:	https://hdl.handle.net/10171/20131
Access Level:	acceso abierto
Palabra clave:	Amidation PAM PHM PAL Rat gastrointestinal tract GLP1 Immunocytochemistry In situ hybridization

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spelling	Localization of amidating enzymes (PAM) in rat gastrointestinal tractMartinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730dBurrell, M.A. (María Ángela)\|\|\|/items/27fe7d6b-4d2b-4917-91af-68de4749f75bKuijk, M. (Marjolein)\|\|\|/items/975bacec-5070-4d15-bb4e-782d3beab9e3Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596Treston, A.M. (Anthony M.)\|\|\|/items/2a2177c8-e6d5-486c-841e-62a5423a037bCuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188Polak, J.M. (Julia M.)\|\|\|/items/27bc2706-e444-4ece-99d6-b2bde0c966b4AmidationPAMPHMPALRat gastrointestinal tractGLP1ImmunocytochemistryIn situ hybridizationWe studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.Histochemical SocietyDadun. Depósito Académico Digital Universidad de Navarra20112011-12-0519931993-01-0119931993-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/20131reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/201312026-06-21T12:47:57Z
dc.title.none.fl_str_mv	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
title	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
spellingShingle	Localization of amidating enzymes (PAM) in rat gastrointestinal tract Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d Amidation PAM PHM PAL Rat gastrointestinal tract GLP1 Immunocytochemistry In situ hybridization
title_short	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
title_full	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
title_fullStr	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
title_full_unstemmed	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
title_sort	Localization of amidating enzymes (PAM) in rat gastrointestinal tract
dc.creator.none.fl_str_mv	Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d Burrell, M.A. (María Ángela)\|\|\|/items/27fe7d6b-4d2b-4917-91af-68de4749f75b Kuijk, M. (Marjolein)\|\|\|/items/975bacec-5070-4d15-bb4e-782d3beab9e3 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Treston, A.M. (Anthony M.)\|\|\|/items/2a2177c8-e6d5-486c-841e-62a5423a037b Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Polak, J.M. (Julia M.)\|\|\|/items/27bc2706-e444-4ece-99d6-b2bde0c966b4
author	Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d
author_facet	Martinez, A. (Alfredo)\|\|\|/items/ce27c09e-454d-4645-986e-713760a3730d Burrell, M.A. (María Ángela)\|\|\|/items/27fe7d6b-4d2b-4917-91af-68de4749f75b Kuijk, M. (Marjolein)\|\|\|/items/975bacec-5070-4d15-bb4e-782d3beab9e3 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Treston, A.M. (Anthony M.)\|\|\|/items/2a2177c8-e6d5-486c-841e-62a5423a037b Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Polak, J.M. (Julia M.)\|\|\|/items/27bc2706-e444-4ece-99d6-b2bde0c966b4
author_role	author
author2	Burrell, M.A. (María Ángela)\|\|\|/items/27fe7d6b-4d2b-4917-91af-68de4749f75b Kuijk, M. (Marjolein)\|\|\|/items/975bacec-5070-4d15-bb4e-782d3beab9e3 Montuenga-Badia, L.M. (Luis M.)\|\|\|/items/4c999705-b2c9-45ac-ba13-3f18594ae596 Treston, A.M. (Anthony M.)\|\|\|/items/2a2177c8-e6d5-486c-841e-62a5423a037b Cuttitta, F. (Frank)\|\|\|/items/0f0da08c-0efe-4d3c-97ca-44c38a26e188 Polak, J.M. (Julia M.)\|\|\|/items/27bc2706-e444-4ece-99d6-b2bde0c966b4
author2_role	author author author author author author
dc.contributor.none.fl_str_mv	Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv	Amidation PAM PHM PAL Rat gastrointestinal tract GLP1 Immunocytochemistry In situ hybridization
topic	Amidation PAM PHM PAL Rat gastrointestinal tract GLP1 Immunocytochemistry In situ hybridization
description	We studied the distribution of the two enzymes involved in post-translational C-terminal alpha-amidation of regulatory peptides in rat digestive tract, using immunocytochemical methods and in situ hybridization techniques. The enzymes were located in most of the fibers and neurons of the myenteric and submucous plexus throughout the entire digestive tract and in endocrine cells of the stomach and colon. Staining of reverse-face serial sections demonstrated that the enzymes in endocrine cells of the stomach co-localized with gastrin in the bottom of the gastric glands. Some gastrin-immunoreactive cells near the neck of the gland were negative for PAM, suggesting that amidation takes place only in the more mature cells. In the colon all cells immunoreactive for glucagon and GLP1 were also positive for peptidylglycine alpha-hydroxylating monooxygenase (PHM) but not for peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). The absence of immunoreactivity for the amidating enzymes in endocrine cells of the small intestine, known to produce C-terminally amidated peptides, suggests the existence of other amidating enzymes.
publishDate	1993
dc.date.none.fl_str_mv	1993 1993-01-01 1993 1993-01-01 2011 2011-12-05
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10171/20131
url	https://hdl.handle.net/10171/20131
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.publisher.none.fl_str_mv	Histochemical Society
publisher.none.fl_str_mv	Histochemical Society
dc.source.none.fl_str_mv	reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra
instname_str	Universidad de Navarra
reponame_str	Dadun. Depósito Académico Digital de la Universidad de Navarra
collection	Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.300719

Localization of amidating enzymes (PAM) in rat gastrointestinal tract

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