Light-photoreceptors and proteins related to Monilinia laxa photoresponses

Light represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three <i>Monilinia laxa</i> isolates. The highest mycelial growth rate was observed under red light for the three &l...

Full description

Bibliographic Details
Authors: Rodríguez-Pires, Silvia, Espeso, Eduardo A., Rasiukevičiūtė, Neringa, Melgarejo, Paloma, De Cal Cortina, Antonieta
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/227875
Online Access:http://hdl.handle.net/10261/227875
Access Level:Open access
Keyword:Photoreceptors
Light response
Monilinia laxa
Brown rot
Photobiology
id ES_d7315c3e21cabdf15ee59ea18a5b0288
oai_identifier_str oai:digital.csic.es:10261/227875
network_acronym_str ES
network_name_str España
repository_id_str
spelling Light-photoreceptors and proteins related to Monilinia laxa photoresponsesRodríguez-Pires, SilviaEspeso, Eduardo A.Rasiukevičiūtė, NeringaMelgarejo, PalomaDe Cal Cortina, AntonietaPhotoreceptorsLight responseMonilinia laxaBrown rotPhotobiologyLight represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three <i>Monilinia laxa</i> isolates. The highest mycelial growth rate was observed under red light for the three <i>M. laxa</i> isolates, followed by green light, daylight or darkness. However, reduced sporulation levels were observed in darkness and red light, but conidiation enhancement was found under daylight, black and green light with more hours of exposure to light. Putative photoreceptors for blue (white-collar and cryptochromes), green (opsins), and red light (phytochromes) were identified, and the photoresponse-related regulatory family of velvet proteins. A unique ortholog for each photoreceptor was found, and their respective domain architecture was highly conserved. Transcriptional analyses of uncovered sets of genes were performed under daylight or specific color light, and both in time course illumination, finding light-dependent triggered gene expression of <i>MlVEL2</i>, <i>MlPHY2</i>, <i>MlOPS2</i>, and <i>MlCRY2</i>, and color light as a positive inductor of <i>MlVEL3</i>, <i>MlVEL4</i>, <i>MlPHY1</i>, and <i>MlCRY1</i> expression. <i>M. laxa</i> has a highly conserved set of photoreceptors with other light-responsive fungi. Our phenotypic analyses and the existence of this light-sensing machinery suggest transcriptional regulatory systems dedicated to modulating the development and dispersion of this pathogen.This study was funded by grants AGL2014-55287-C2-1-R, RTI2018-094263-B-I00, and AGL2017-84389-C2-2-R from the Ministry of Science, Innovation and Universities (MCIU, Spain), Agencia Estatal de Investigación (AEI), and the Fondo Europeo de Desarrollo Regional (FEDER, EU). S. Rodríguez-Pires received a Ph.D. fellowship from the Ministry of Science, Innovation and Universities (Spain)Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/227875reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-55287-C2-1-Rinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094263-B-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84389-C2-2-RSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2278752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Light-photoreceptors and proteins related to Monilinia laxa photoresponses
title Light-photoreceptors and proteins related to Monilinia laxa photoresponses
spellingShingle Light-photoreceptors and proteins related to Monilinia laxa photoresponses
Rodríguez-Pires, Silvia
Photoreceptors
Light response
Monilinia laxa
Brown rot
Photobiology
title_short Light-photoreceptors and proteins related to Monilinia laxa photoresponses
title_full Light-photoreceptors and proteins related to Monilinia laxa photoresponses
title_fullStr Light-photoreceptors and proteins related to Monilinia laxa photoresponses
title_full_unstemmed Light-photoreceptors and proteins related to Monilinia laxa photoresponses
title_sort Light-photoreceptors and proteins related to Monilinia laxa photoresponses
dc.creator.none.fl_str_mv Rodríguez-Pires, Silvia
Espeso, Eduardo A.
Rasiukevičiūtė, Neringa
Melgarejo, Paloma
De Cal Cortina, Antonieta
author Rodríguez-Pires, Silvia
author_facet Rodríguez-Pires, Silvia
Espeso, Eduardo A.
Rasiukevičiūtė, Neringa
Melgarejo, Paloma
De Cal Cortina, Antonieta
author_role author
author2 Espeso, Eduardo A.
Rasiukevičiūtė, Neringa
Melgarejo, Paloma
De Cal Cortina, Antonieta
author2_role author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Photoreceptors
Light response
Monilinia laxa
Brown rot
Photobiology
topic Photoreceptors
Light response
Monilinia laxa
Brown rot
Photobiology
description Light represents a ubiquitous source of information for organisms to evaluate their environment. The influence of light on colony growth and conidiation was determined for three <i>Monilinia laxa</i> isolates. The highest mycelial growth rate was observed under red light for the three <i>M. laxa</i> isolates, followed by green light, daylight or darkness. However, reduced sporulation levels were observed in darkness and red light, but conidiation enhancement was found under daylight, black and green light with more hours of exposure to light. Putative photoreceptors for blue (white-collar and cryptochromes), green (opsins), and red light (phytochromes) were identified, and the photoresponse-related regulatory family of velvet proteins. A unique ortholog for each photoreceptor was found, and their respective domain architecture was highly conserved. Transcriptional analyses of uncovered sets of genes were performed under daylight or specific color light, and both in time course illumination, finding light-dependent triggered gene expression of <i>MlVEL2</i>, <i>MlPHY2</i>, <i>MlOPS2</i>, and <i>MlCRY2</i>, and color light as a positive inductor of <i>MlVEL3</i>, <i>MlVEL4</i>, <i>MlPHY1</i>, and <i>MlCRY1</i> expression. <i>M. laxa</i> has a highly conserved set of photoreceptors with other light-responsive fungi. Our phenotypic analyses and the existence of this light-sensing machinery suggest transcriptional regulatory systems dedicated to modulating the development and dispersion of this pathogen.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/227875
url http://hdl.handle.net/10261/227875
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-55287-C2-1-R
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094263-B-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-84389-C2-2-R
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420979506118656
score 15,811543

Similar Items