In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)

Herein, a supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes under the following conditions: a temperature of 40 °C, pressure of 225 bar, reaction time of 1-5 h, and the flow rate of 20 L h-1 for recirculated...

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Autores: Morales Hernández, Diego, Tejedor Calvo, Eva, Jurado-Chivato, Noelia, Polo, Gonzalo, Tabernero, María, Largo, Carlota, Ruiz Rodríguez, Alejandro, Soler Rivas, Cristina
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710961
Acceso en línea:http://hdl.handle.net/10486/710961
https://dx.doi.org/10.1039/c9fo01744e
Access Level:acceso abierto
Palabra clave:Animals
beta-Glucans
Cholesterol
Ergosterol
Fruiting Bodies, Fungal
Male
Mice
Mice, Inbred C57BL
Shiitake Mushrooms
Ciencia y Tecnología de Alimentos
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spelling In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)Morales Hernández, DiegoTejedor Calvo, EvaJurado-Chivato, NoeliaPolo, GonzaloTabernero, MaríaLargo, CarlotaRuiz Rodríguez, AlejandroSoler Rivas, CristinaAnimalsbeta-GlucansCholesterolErgosterolFruiting Bodies, FungalMaleMiceMice, Inbred C57BLShiitake MushroomsCiencia y Tecnología de AlimentosHerein, a supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes under the following conditions: a temperature of 40 °C, pressure of 225 bar, reaction time of 1-5 h, and the flow rate of 20 L h-1 for recirculated CO2. Moreover, ergosterol (ERG) and the SFE extract (SFE) with highest ergosterol concentration were microemulsified and submitted to in vitro digestion to study their ability to displace cholesterol from dietary mixed micelles (DMMs). ERG was also mixed with a β-glucan-enriched (33.5%) extract (BGE) obtained from L. edodes to investigate the synergies between them; the results indicated that all these extracts (including BGE without ERG) could reduce the cholesterol levels in the DMMs. However, when ERG and SFE were simultaneously administered to mice with a hypercholesterolemic diet, no significant differences in the serum cholesterol levels were detected as compared to the case of the control. However, when only BGE was administrated to another mice model previously induced with hypercholesterolemia, significant reduction in the cholesterol levels was noticedThis research was supported by the national R+D program from the Spanish Ministry of Science and Innovation (project AGL2014-26211-R) and the regional program from the Community of Madrid, Spain (S2013/ABI-2728).Royal Society of ChemistryFacultad de Ciencias20192019-11-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710961https://dx.doi.org/10.1039/c9fo01744ereponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7109612026-06-23T12:46:27Z
dc.title.none.fl_str_mv In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
title In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
spellingShingle In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
Morales Hernández, Diego
Animals
beta-Glucans
Cholesterol
Ergosterol
Fruiting Bodies, Fungal
Male
Mice
Mice, Inbred C57BL
Shiitake Mushrooms
Ciencia y Tecnología de Alimentos
title_short In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
title_full In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
title_fullStr In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
title_full_unstemmed In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
title_sort In vitro and in vivo testing of the hypocholesterolemic activity of ergosterol- and β-glucan-enriched extracts obtained from shiitake mushrooms (Lentinula edodes)
dc.creator.none.fl_str_mv Morales Hernández, Diego
Tejedor Calvo, Eva
Jurado-Chivato, Noelia
Polo, Gonzalo
Tabernero, María
Largo, Carlota
Ruiz Rodríguez, Alejandro
Soler Rivas, Cristina
author Morales Hernández, Diego
author_facet Morales Hernández, Diego
Tejedor Calvo, Eva
Jurado-Chivato, Noelia
Polo, Gonzalo
Tabernero, María
Largo, Carlota
Ruiz Rodríguez, Alejandro
Soler Rivas, Cristina
author_role author
author2 Tejedor Calvo, Eva
Jurado-Chivato, Noelia
Polo, Gonzalo
Tabernero, María
Largo, Carlota
Ruiz Rodríguez, Alejandro
Soler Rivas, Cristina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias
dc.subject.none.fl_str_mv Animals
beta-Glucans
Cholesterol
Ergosterol
Fruiting Bodies, Fungal
Male
Mice
Mice, Inbred C57BL
Shiitake Mushrooms
Ciencia y Tecnología de Alimentos
topic Animals
beta-Glucans
Cholesterol
Ergosterol
Fruiting Bodies, Fungal
Male
Mice
Mice, Inbred C57BL
Shiitake Mushrooms
Ciencia y Tecnología de Alimentos
description Herein, a supercritical extraction plant (with a 6 L extraction cell) was successfully used to obtain ergosterol-enriched extracts from Lentinula edodes under the following conditions: a temperature of 40 °C, pressure of 225 bar, reaction time of 1-5 h, and the flow rate of 20 L h-1 for recirculated CO2. Moreover, ergosterol (ERG) and the SFE extract (SFE) with highest ergosterol concentration were microemulsified and submitted to in vitro digestion to study their ability to displace cholesterol from dietary mixed micelles (DMMs). ERG was also mixed with a β-glucan-enriched (33.5%) extract (BGE) obtained from L. edodes to investigate the synergies between them; the results indicated that all these extracts (including BGE without ERG) could reduce the cholesterol levels in the DMMs. However, when ERG and SFE were simultaneously administered to mice with a hypercholesterolemic diet, no significant differences in the serum cholesterol levels were detected as compared to the case of the control. However, when only BGE was administrated to another mice model previously induced with hypercholesterolemia, significant reduction in the cholesterol levels was noticed
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-11-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710961
https://dx.doi.org/10.1039/c9fo01744e
url http://hdl.handle.net/10486/710961
https://dx.doi.org/10.1039/c9fo01744e
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869420958615339008
score 15,300719

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