Synthesis, DNA intercalation and europium(III)-triggered DNA photocleavage by a bis-proflavine succinamide conjugate

We describe the synthesis of a bis-proflavine derivative containing a succinamide linking chain. Levels of pUC19 plasmid DNA photocleavage by this compound are significantly enhanced in the presence of Eu3+ (350 nm, 22 degreesC, pH 7.0). UV-visible spectrophotometric Studies of the ligand With Calf...

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Detalles Bibliográficos
Autores: Espinosa Merino, Juan Félix, Fernández Domínguez, María José, Grant, Kathryn B., Gude Rodríguez, Lourdes|||0000-0002-0220-3755, Rodrigo López, María Melia|||0000-0001-9787-0843, Lorente Pérez, Antonio
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/65014
Acceso en línea:http://hdl.handle.net/10017/65014
https://dx.doi.org/10.1016/j.tetlet.2004.03.177
Access Level:acceso abierto
Palabra clave:Química
Chemistry
Descripción
Sumario:We describe the synthesis of a bis-proflavine derivative containing a succinamide linking chain. Levels of pUC19 plasmid DNA photocleavage by this compound are significantly enhanced in the presence of Eu3+ (350 nm, 22 degreesC, pH 7.0). UV-visible spectrophotometric Studies of the ligand With Calf thymus DNA show bathochromic-shifts and hypochromicity in the major absorption bands of the bis-proflavine derivative. Viscosimetric analysis of the helical extension of sonicated calf thymus DNA agrees with a monofunctional intercalation process. (C) 2004 Elsevier Ltd. All rights reserved.